Objective To explore the dynamic changes of collagen type Ⅰand Ⅳ messenger ribonucleic acid(mRNA)expression in the lung tissue of neonatal rats after inhaling high concentration of oxygen and the role of collagen type Ⅰand Ⅳ mRNA in chronic lung disease(CLD)induced by hyperoxia.Methods Full-term newborn rats were grouped according to inhale the concentration of oxygen into hypero-xia group and air control group after birth within 12 hours.Lung histological section at day 1,3,7,14 and 21 in 2 groups were prepared for hematoxylin-eosin staining and the detection of mRNA level of collagen type Ⅰand Ⅳ by in situ hybridization.The results were analyzed with SPSS 11.0 software.Results Compared with air control group,inflammation response was seen in early stage,the arrest of lung development was evident after 7 d of oxygen exposure,at last interstitial fibrosis.It was shown that the positive expression of collagen typeⅠ was mainly in the alveolar epithelial cells and endothelial cells by in situ hybridization.The expression of collagen typeⅠ mRNA was weakened compared to air group on 7 d(P0.05).Conclusions Prolonged hyperoxia may cause the onset of arrested lung development and lung fibrosis,which are similar to the changes of chronic lung disease.The collagen type Ⅰand Ⅳ mRNA expressions are not parallel to their protein contents,suggesting the main modulation of these collagens may be not at transcriptional level.

Objective To investigate insulin resistance and the effect of physical training on it in the pa- tients with chronic heart failure (CHF). Methods One hundred and twenty NYHAⅡ-ⅢCHF patients were ran- domly divided into a training group( n = 65 ) and a routine therapy group (n = 55 ). Another 35 healthy subjects were recruited as control group. All the patients were treated with routine anti-CHF drugs, and the training group patients had received physical training twice a day in addition. The HOMA-IR, insulin sensitivity index (ISI) , left ventricu- lar ejection fraction (LEVF), left ventricular fractional shortening( LVFS), 6-minute walking distance, heart rate and mean blood pressure were compared between the training and routine therapy groups before and after physical ex- ercise in both groups, and a comparison was made between the patients and the controls before the intervention with regard to HOMA-IR and ISI. Results Comparing with control group, ISI was reduced while the HOMA-IR in- creased (P

OBJECTIVETo explore the health status and related influencing factors for 3-year-old children in rural area of Anhui province.

METHODSClustered random sampling method was used to investigate the general situation of the children and their families as well as to measure height, weight and some other indexes of the children. Health status and its influencing factors were then evaluated.

RESULTSA total number of 418 children were investigated and incidence rates of growth retardation, underweight, overweight, emaciation and obesity appeared to be 4.3%, 1.9%, 0.7%, 0.5% and 14.9% respectively. Z value of development index was high among children with the following situations: heavy birth weight, father was tall, being boys, and having habits as washing hands before meals. However, Z value was low among kids with following characteristics: being the youngest among all the siblings, family income was high, mothers were illiterate as well as taking tocolytic agent and exposure to X-rays during pregnancy, delivered at home, major care taker was mother, mixed feeding in the early 4-month-old, age of supplementary food was less than 3 months, being picky on foods and taking snacks frequently etc.

CONCLUSIONHealth status of the 3-year-old children in rural area of Anhui province was fine in general, however, more attentionn needs to be paid to pregnant women and child health care takers in the rural areas.

Adult ; Child, Preschool ; China ; Female ; Health Status ; Humans ; Male ; Maternal Exposure ; Pregnancy ; Rural Health

Objective To study the bactericidal efficacy of hospital commonly used disinfectants such as ethanol,3" chlorine tablets,iodophor,glutaraldehyde,and avagard instant hand antiseptic on Staphylococcus aureus (S.a ureus) from different sources of hospital,and provide scientific basis for effective control of healthcare-associated infection(HAI).Methods A total of 48 strains of S.aureus from inpatients,hands of health care workers,and environment surface in the First Affiliated Hospital of Nanchang University were collected.Disinfectant was directly contacted with bacteria,in vitro killing efficacy of disinfectant on S.aureus from different sources at different diluted concentrations,and different contact time were studied.Results The killing rate of 5g/L iodophor,20g/L glutaraldehyde,and avagard instant hand antiseptic(0.5％ chlorhexidine + 70％ ethanol) to S.aureus with a 5-minute contact time was 100％;killing rates of 70％ ethanol and 1g/L 3" chlorine tablets to S.aureus with a 5-minute contact time were 96.5 ％-99.8 ％;but highly diluted iodophor,glutaraldehyde,and avagard instant hand antiseptic still could not completely kill S.aureus even the contact time was extended.Conclusion The routine use of disinfectants in the hospital can meet the clinical bactericidal efficacy,it is necessary to monitor concentration routinely,avoid decreasing sterilization ability.

Humans ; Infant ; Female ; Dandy-Walker Syndrome/diagnosis* ; Tomography, X-Ray Computed ; Craniocerebral Trauma/complications*

PURPOSE: To develop a simple and reliable rat model of in situ reversible obstructive jaundice with low morbidity and mortality rates. METHODS: Rats were divided into 4 groups with 8 rats each: the sham-operated (SH) group only underwent laparotomy, the control internal drainage (ID-C) group underwent choledochoduodenostomy, the new internal drainage (ID-N) group and the long-term internal drainage (ID-L) group underwent choledochocholedochostomy. Common bile duct ligation was performed in all the drainage groups 7 days before reversal procedures. All rats were sacrificed for samples 7 days after the last operation except rats of the ID-L group that survived 28 days before sacrifice. Body weight, liver function, histopathological changes, morbidity and mortality were assessed. RESULTS: One rat died and 2 rats had complications with tube blockage in the ID-C group. No death or complications occurred in the ID-N and ID-L groups. The drainage tube remained patent in the long-term observation ID-L group. Body weight showed no significant difference between the ID-C and ID-N groups after 7 days drainage. Liver function was not fully recovered in the ID-C and ID-N groups after 7 days drainage, but statistical differences were only observed in the ID-C group compared with the SH and ID-L groups. Periportal inflammation and bile duct proliferation showed severer in the ID-C group than in the ID-N group. CONCLUSION: The present study provided an efficient, simple, and reliable rat model that is especially suitable for long-term or consecutive studies of reversible obstructive jaundice.
Animals ; Bile Ducts ; Body Weight ; Choledochostomy ; Common Bile Duct ; Drainage ; Inflammation ; Jaundice, Obstructive* ; Laparotomy ; Ligation ; Liver ; Models, Animal* ; Mortality ; Rats*

PURPOSE: To develop a simple and reliable rat model of in situ reversible obstructive jaundice with low morbidity and mortality rates. METHODS: Rats were divided into 4 groups with 8 rats each: the sham-operated (SH) group only underwent laparotomy, the control internal drainage (ID-C) group underwent choledochoduodenostomy, the new internal drainage (ID-N) group and the long-term internal drainage (ID-L) group underwent choledochocholedochostomy. Common bile duct ligation was performed in all the drainage groups 7 days before reversal procedures. All rats were sacrificed for samples 7 days after the last operation except rats of the ID-L group that survived 28 days before sacrifice. Body weight, liver function, histopathological changes, morbidity and mortality were assessed. RESULTS: One rat died and 2 rats had complications with tube blockage in the ID-C group. No death or complications occurred in the ID-N and ID-L groups. The drainage tube remained patent in the long-term observation ID-L group. Body weight showed no significant difference between the ID-C and ID-N groups after 7 days drainage. Liver function was not fully recovered in the ID-C and ID-N groups after 7 days drainage, but statistical differences were only observed in the ID-C group compared with the SH and ID-L groups. Periportal inflammation and bile duct proliferation showed severer in the ID-C group than in the ID-N group. CONCLUSION: The present study provided an efficient, simple, and reliable rat model that is especially suitable for long-term or consecutive studies of reversible obstructive jaundice.
Animals ; Bile Ducts ; Body Weight ; Choledochostomy ; Common Bile Duct ; Drainage ; Inflammation ; Jaundice, Obstructive* ; Laparotomy ; Ligation ; Liver ; Models, Animal* ; Mortality ; Rats*

BACKGROUNDLaparoendoscopic single-site surgery radical prostatectomy (LESS-RP) is a challenging urological procedure and needs to be further evaluated. This study was undertaken to illustrate the safety and initial results of pure LESS-RP with conventional available instruments.

METHODSA prospective clinical database was established in September 2010 to assess the outcome following the introduction of LESS-RP at our institution. By June 2012, six procedures had been performed. The mean patient age was 74.7 (74.0 - 76.0) years. The mean body mass index (BMI) was 23.8 (19.5 - 32.2) kg/m(2). The LESS-RP was preformed through an extra-peritoneal approach using single port access with QudaPort, 0° lens 5 mm flexible tip video-laparoscope and available conventional laparoscopic instruments. Parameters assessed were operative time, estimated blood loss, intra-operative complications, drainage time, postoperative pain score (visual analogue pain scale (VAPS), 0 - 10), pathological results, and postoperative prostate specific antigen (PSA) levels.

RESULTSLESS-RP was completed for all six cases without additional trocar placement or conversion to standard laparoscopic or open radical prostatectomy. The mean operative times were 252.5 (190.0 - 305.0) minutes, estimated blood loss was 300 (100 - 500 ml). There was no documentable intraoperative complication. The mean wound-drainage time was 5.2 (2.0 - 7.0) days, and the first postoperative day VAPS was 0.8 (0 - 3.0). Final pathological staging was pT2aN0M0 in four cases and pT2cN0M0 in two cases. Surgical margins were negative for all cases. The one-month post-operative PSA was less than 0.02 ng/ml in each case. All patients were continent without pad usage at 1 year postoperatively.

CONCLUSIONThe technique of pure LESS-RP is feasible and early outcomes are acceptable.

Aged ; Humans ; Laparoscopy ; methods ; Male ; Prospective Studies ; Prostate-Specific Antigen ; blood ; Prostatectomy ; methods ; Prostatic Neoplasms ; blood ; surgery ; Treatment Outcome

OBJECTIVETo examine the association between polymorphism of vascular endothelial growth factor(VEGF)1498 C/T,936 C/T and colorectal adenoma genetic susceptibility.

METHODSA case-control study of 224 colorectal adenomas and 200 controls was conducted and VEGF genotypes were determined based on TaqMan-probe assay. The epidemiological factors were collected through questionnaire. Accordingly, the clinicopathological data of each sample were also investigated.

RESULTSThe carriage of 936 CT and CT+TT genotypes had significantly higher risk of colorectal adenoma (CT vs. CC, OR=2.00, 95% CI: 1.23-3.25, P=0.006; CT+TT vs. CC, OR=2.04, 95% CI:1.28-3.26, P=0.003). 936-T allele carriage had increased risk of colorectal adenoma (OR=1.91, 95% CI:1.25-2.91, P=0.003). The genotypes of 1498 C/T and the frequency of C/T allele showed no differences between healthy persons and patients (P>0.05). In patients with 936 CT+TT and 936-T allele implied a tendency of villous adenoma category (CT+TT vs. CC, OR=2.54, 95% CI:1.12-5.75, P=0.040; T allele vs. C allele, OR=3.08, 95% CI, 1.64-5.80, P=0.001).

CONCLUSIONVEGF 936 C/T polymorphism can influence susceptibility to colorectal adenoma.

Adenoma ; genetics ; Adult ; Case-Control Studies ; Colorectal Neoplasms ; genetics ; Female ; Genetic Predisposition to Disease ; Genotype ; Humans ; Middle Aged ; Polymorphism, Single Nucleotide ; Vascular Endothelial Growth Factor A ; genetics

Adenine ; analogs & derivatives ; therapeutic use ; Adolescent ; Adult ; Aged ; Creatinine ; blood ; Female ; Hepatitis B, Chronic ; blood ; drug therapy ; Humans ; Male ; Middle Aged ; Organophosphonates ; therapeutic use ; Phosphorus ; blood ; Retrospective Studies ; Young Adult