2.Application and future prospect of 18F-FLT PET-CT in guiding delineation of biological target volume.
Da-li HAN ; Wan-rong JIANG ; Jin-ming YU
Chinese Journal of Oncology 2009;31(1):1-4
Dideoxynucleosides
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False Positive Reactions
;
Fluorine Radioisotopes
;
Fluorodeoxyglucose F18
;
Humans
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Inflammation
;
diagnosis
;
Neoplasm Staging
;
Neoplasms
;
diagnosis
;
metabolism
;
pathology
;
radiotherapy
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Positron-Emission Tomography
;
methods
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Radiotherapy, Intensity-Modulated
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Sensitivity and Specificity
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Tomography, X-Ray Computed
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Treatment Outcome
4.Recent advance on LFA-1/ICAM-1 costimulatory signal of T cell-review.
Wen-Rong HUANG ; Li-Sheng WANG ; Wan-Ming DA
Journal of Experimental Hematology 2004;12(4):533-537
LFA-1/ICAM-1 costimulation plays an important role in immunologic reaction of many different T cell populations. After TCR/CD3 complex cross-linking MHC/peptide, LFA-1, expressed on T cell increases a higher affinity and avidity for ICAM-1 rapidly. LFA-1 is a key molecule in formation of the immune synapse. LFA-1/ICAM-1 costimulation can engage various signaling events of T cell by up-regulating the activities of PI 3-kinase, sphingomyelinase, and c-Jun NH2-terminal kinase. With the costimulation of LFA-1/ICAM-1, engagement of TCR molecules results in a significant increase of T cell activities, including higher Th1 cytokines production, strongly proliferative response and higher T cell cytotoxicity.
Animals
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Cytokines
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biosynthesis
;
Cytotoxicity, Immunologic
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Humans
;
Intercellular Adhesion Molecule-1
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physiology
;
Lymphocyte Activation
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Lymphocyte Function-Associated Antigen-1
;
physiology
;
Signal Transduction
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T-Lymphocytes
;
immunology
5.Non-Myeloablative Allogeneic Hematopoietic Stem Cell Transplantation-New Opinion about Hematopoietic Stem Cell Transplantation
Journal of Experimental Hematology 2001;9(1):80-85
Non-myeloablative allogeneic hematopoietic stem cell transplantation (NMAT) is brought forward recent years. It is a modified transplant technology. NMAT treats malignant disease by graft versus tumor effect of chimerism after transplantation. The difference between this transplantation and standard allogeneic stem cell transplantation is the conditioning regimen. Non-myeloablative allogeneic stem cell transplantation increased the indication of allogeneic stem cell transplantation. This article focused on the background of this transplantation, the results of animal experimental and clinical research, the advantages and shortages of this transplantation.
6.The approaches of chronic graft-versus-host disease.
Journal of Experimental Hematology 2003;11(2):213-216
Chronic graft-versus-host disease (cGVHD) continues to be a major complication in long-term survivors after allogeneic hematopoietic stem cell transplantation and is the principal cause of morbidity and non-relapse mortality. With the new approaches in the immune mechanisms of cGVHD, the diagnosis, prophylaxis and treatment of cGVHD are involved. This review summarises the current progress of research on cGVHD.
Chronic Disease
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Female
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Graft vs Host Disease
;
diagnosis
;
etiology
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therapy
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Humans
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Male
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Prognosis
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Risk Factors
7.Effects of rhG-CSF on T cell during peripheral blood stem/progenitor cell mobilization and its mechanisms--review.
Journal of Experimental Hematology 2005;13(2):338-342
Recombinant human granulocyte-colony-stimulating factor (rhG-CSF) can widely regulate human immunologic response. In the protocol of peripheral blood stem/progenitor cell mobilization, rhG-CSF can change the numbers and functions of T cells. Then the results can impact the incidence of graft-versus-host disease after allogeneic peripheral blood stem/progenitor cell transplantation. The regulation of rhG-CSF on T cell is an indirect action which is based on the direct action to monocytes and dendritic cells. The numerous IL-10 secreted by monocytes plays a key role in cytokines production, proliferative response and cytotoxicity of T cells. Endogenous IL-10 can induce high expression of SOCS3 and the SOCS3 is very important for regulating the signal transduction of the activities of T cells. In this review influences of rhG-CSF on T-cells in mobilization process and related mechanisms were elaborated with emphasis.
Blood Donors
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Granulocyte Colony-Stimulating Factor
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pharmacology
;
therapeutic use
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Hematopoietic Stem Cell Mobilization
;
methods
;
Humans
;
Interleukin-10
;
biosynthesis
;
Recombinant Proteins
;
Suppressor of Cytokine Signaling 3 Protein
;
Suppressor of Cytokine Signaling Proteins
;
biosynthesis
;
T-Lymphocytes
;
cytology
;
drug effects
;
metabolism
8.In vitro biological characteristics of mesenchymal stem cells from patients with myelodysplastic syndrome and their support to hematopoiesis.
Journal of Experimental Hematology 2005;13(5):839-842
To study the biological characteristics of mesenchymal stem cells (MSC) from patients with myelodysplastic syndrome (MDS) and their supportive capacity for hematopoiesis in vitro, MSCs from bone marrow samples of MDS patients were isolated, cultured and expanded. Morphology, immunophenotype, osteoblasts differentiative and proliferative property of MSC and colony forming unit-fibroblast (CFU-F) were measured and analyzed. Mononuclear cells (MNC) of cord blood were plated onto a feeder layer formed by MSC of MDS patient, cells count and CFU-GM production were observed. The results showed that the culture-expanded cells from MDS patients presented a typical fibroblast-like morphology. Cells were positive for SH2 (CD105), SH3 (CD73), Thy-1 (CD90), but negative for CD34 and CD45. After induction, these cells could differentiate into osteoblasts. Their proliferative capacity and CFU-F number were similar to those of MSC from healthy donors. The total cell count and CFU-GM yield in supernatants after culture for 2 weeks were significantly lower than those of control in hematopoiesis supportive experiments in vitro (P < 0.05). It is concluded that the biological characteristics of MSC from bone marrow of MDS patients are not different from those of MSC isolated from bone marrow of normal donors, however, their capacity of hematopoiesis support in vitro are significantly weaker.
5'-Nucleotidase
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analysis
;
Adult
;
Aged
;
Antigens, CD
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analysis
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Antigens, CD34
;
analysis
;
Bone Marrow Cells
;
cytology
;
immunology
;
Cell Differentiation
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Endoglin
;
Female
;
Hematopoiesis
;
Humans
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Male
;
Mesenchymal Stromal Cells
;
cytology
;
immunology
;
Middle Aged
;
Myelodysplastic Syndromes
;
blood
;
Receptors, Cell Surface
;
analysis
9.Regulation of immunity by sphingosine 1-phosphate and its G protein-coupled receptors--review.
Journal of Experimental Hematology 2007;15(6):1317-1324
Sphingosine 1-phosphate (S1P) is an important biologically active lysophospholipid that transmits signals through a family of G-protein-coupled receptors (GPCRs) to regulate the vital functions of several types of immune cells. The S1P GPCRs suppress both generation of specialized functional cytokines, such as IFN-gamma and IL-4, and proliferation of T-cells. Although S1P is chemotactic to T cells, B cells, dendritic cells, and natural killer cells, the major effect of S1P on the immune system is the regulation of lymphocyte recirculation and tissue distribution by S1P and S1P1. Chemotactic response of CD4+CD25+ regulatory T cells to S1P is reduced, but its optimal suppressive activities require S1P. FTY720, a new class of immunomodulator, is rapidly phosphorylated by sphingosine kinase 2 in vivo to form the biologically active phosphorylated-FTY720 (FTY720-P), which closely resembles S1P. The FTY720-P is a true agonist for S1P1, S1P3, S1P4, and S1P5, it affects the tissue distribution and functional activity of T cells, B cells, dendritic cells and regulatory T cells. FTY720 were demonstrated to be a hypotoxic, great effective and reversible immunosuppressive efficacy to prevent allograft rejection and treat some autoimmune diseases. In this article, the synthesis and metabolism of S1P, the expression of S1P GPCRs in immune cells, the effect of S1P on immune cells, the drugs targeted to S1P GPCRs and their clinical implications are reviewed.
Fingolimod Hydrochloride
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Humans
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Immunomodulation
;
physiology
;
Lysophospholipids
;
immunology
;
physiology
;
Propylene Glycols
;
metabolism
;
Receptors, G-Protein-Coupled
;
immunology
;
physiology
;
Sphingosine
;
analogs & derivatives
;
immunology
;
metabolism
;
physiology
10.Construction of ICAM-1-GFP and its binding with Molt-4 cells.
Wei-Hua CHEN ; Wan-Ming DA ; Chun-Ji GAO
Journal of Experimental Hematology 2009;17(3):650-655
This study was aimed to clone human intercellular adhesion molecule-1 (ICAM-1) gene, to transfect the constructed eukaryotic expression vector ICAM-1-GFP into CHO cells, as well as to detect ICAM-1-GFP expression in CHO cells binding with Molt-4 cells. ICAM-1 cDNA gene was amplified by RT-PCR and inserted in PMD(18)-T vector. Then ICAM-1 cDNA from pMD18-ICAM-1 vector was subcloned into eukaryotic expression vector pEGFP-C1 to construct recombinant ICAM-1-pEGFP-C1 vector. Restriction analysis and DNA sequencing were used to confirm the recombinant vector. After stable transfection of CHO-K1 cells with the recombinant vector, the expression and subcellular localization of ICAM-1-GFP were detected by RT-PCR, flow cytometry and fluorescence microscopy. The function of ICAM-1-GFP fusion protein was assessed by the binding of ICAM-1-GFP/CHO cells to Molt-4 cells. The results showed that 1622 bp full-length ICAM-1 cDNA obtained and was successfully ligated with pMD(18)-T-vector, subcloned to construct recombinant ICAM-1-pEGFP-C1 vector. Restriction analysis and DNA sequencing indicated that recombinant ICAM-1-GFP was successfully constructed and ICAM-1-GFP was expressed stably in CHO cells. ICAM-1-GFP expression was only observed in the cytoplasm of ICAM-1-GFP/CHO cells by fluorescence microscopy. The ICAM-1-GFP/CHO cells were bound to PMA-treated Molt-4 cells. The expression of MEM-148 was very weak in PMA-treated Molt-4 cells. It is concluded that the ICAM-1-GFP eukaryotic expression vector has been constructed successfully and expresses stably in CHO cells. PMA can increase the binding of Molt-4 cells to ICAM-1-GFP/CHO cells by inducing specialized form of ICAM-1 clustering.
Animals
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CHO Cells
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Cricetinae
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Cricetulus
;
DNA, Complementary
;
genetics
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Genetic Vectors
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Green Fluorescent Proteins
;
genetics
;
Humans
;
Intercellular Adhesion Molecule-1
;
genetics
;
Recombinant Proteins
;
genetics
;
Transfection