1.Establishment of animal model with gastric hot syndrome.
Wan-bin YANG ; Bin WEN ; Ling-hang ZHANG ; Hong LIU
China Journal of Chinese Materia Medica 2015;40(18):3644-3649
This study is to establish the gastric hot model of rats. After gastric feeding with ethanol solution for 3 weeks and feeding with extra capsaicin and ethanol solution for another 2 weeks, model group show distinct physical sign of gastric hot syndrome. The pathology of gastrics reveals gastricism of model group, while treatment group (treat with Zuojin Wan) shows mild lesion. Elisa detection of model group show that the solution of interleukin-2 (IL-2) is higher than the blank group. The obvious difference among model group, treatment group and blank group reveals the success of the establishment of gastric hot model.
Animals
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Disease Models, Animal
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Drugs, Chinese Herbal
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administration & dosage
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Eating
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Female
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Humans
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Male
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Rats
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Rats, Wistar
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Stomach Diseases
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drug therapy
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pathology
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physiopathology
2.Establishment of animal model with gastric cold syndrome.
Wan-bin YANG ; Bin WEN ; Ling-hang ZHANG ; Hong LIU
China Journal of Chinese Materia Medica 2015;40(20):4031-4036
This study is to establish the gastric cold model of rats. After gastric feeding with cold water for 5 weeks and extra iced water bath in the last 2 weeks, model group show distinct physical sign of gastric cold syndrome. The pathology of gastrics reveals gastricism of model group, while treatment group(treated with Fanzuojin Wan) show mild lesion. Elisa detection of model group show that the solution of interleukin-2 (IL-2) is higher than blank group. The difference with significance among model group, treatment group and blank group reveals the success of the establishment of gastric cold syndrome.
Animals
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Cold Temperature
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Disease Models, Animal
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Female
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Humans
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Male
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Rats
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Rats, Wistar
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Stomach
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chemistry
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metabolism
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pathology
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physiopathology
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Stomach Diseases
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metabolism
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pathology
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physiopathology
3.Effects of salmeterol and fluticasone propionate combined montelukast in the treatment of patients with bronchial asthma
Haiying LU ; Quan YUAN ; Xiaodong YANG ; Xiaobing WAN ; Bin LI
Drug Evaluation Research 2017;40(6):828-831
Objective To compare the effects of salmeterol and fluticasone propionate combined montelukastin the treatment of patients with bronchial asthma.Methods Ninety-eight bronchial asthma patients from April 2014 to March 2016 in the Fourth People's Hospital Sichuan Province were selected anddivided into observation group and control group with 49 patients in each group according to the above principles by prospective study.The control group was given salmeterol treatment,and the observation group was added given montelukast treatment based on the control group.The treatment days were 15 d recorded the prognosis of the two groups.Results The total effective rates of the observation group and the control group were 95.9% and 81.6% respectively,the total effective rate of the observation group was significantly higher than that of the control group (P < 0.05).The FEV1 and FVC values after treatment in the observation group and control group were significantly higher than before treatment (P < 0.05),while the FEV1 and FVC values in the observation group after treatment were significantly higher than those in the control group (P < 0.05).The scores of ACT scale after treatment of two groups were obviously higher than before treatment,and the score of observation group was statistically higher than control group after treatment (P < 0.05).During treatment,there was no statistical significance on adverse reaction between two groups.Conclusion Salmeterol and fluticasone propionate combined montelukast in the treatment of patients with bronchial asthma can promote the improvement of lung function,so as to improve the quality of life and clinical treatment.
4.Fault diagnosis of large-scale medical equipment
Bin YANG ; Shengchun WAN ; Chenxi ZHANG ; Wei FANG
Chinese Medical Equipment Journal 1989;0(02):-
Large-scale medical equipment is very important for comprehensive hospitals. Fault monitoring and diagnosing technologies for large-scale medical equipment have to been available to make full use of them. This paper introduces the evolution of equipment fault diagnosis. After comparing conventional technology and intelligent one, this paper lays emphasis on the latter.
5.The effects of mouth rinse containing 1 g/L cetylpyridinium chloride in the treatment of simple gingivitis
Junzheng WU ; Wei ZHOU ; Bin LIU ; Ling WAN ; Honglin YANG
Journal of Practical Stomatology 1995;0(04):-
0.05). On day 4, GI, SBI and OS were lower than those on day 1 (P0.05). On day 8, the parameters were lower than those on day 4(P0.05). Conclusion: 1 g/L cetylpyridinium chloride is effective in the treatment of simple gingivitis.
6.Comparison of the effect between octreotide combined with omeprazole and omeprazole alone in the treament of upper gastrointestinal bleeding
Kunting DU ; Qinli GE ; Shunmei WAN ; Weijie YANG ; Bin LI ; Yujie YANG
Chinese Journal of Primary Medicine and Pharmacy 2013;20(4):528-530
Objective To compare the effect of octreotide combined with omeprazole and omeprazole alone in the treament of upper gastrointestinal bleeding.Methods Clinical data of 101 patients with gastrointesinal bleeding were retrospectively analyzed.According to the treatment,the patients were divided into the observation group(56 cases) and control group(45 cases).The patients in observation group were given octreotide and omeprazole,and patients in control group were given omeprazol alone.Then the results of the two groups were analyzed.Results The total effective rate of observation group was 91.07%,and was higher than that of the control group(73.33%) (x2 =5.61,P <0.05) ;The average hemostasis time of observation group was (15.9 ±3.9)h,and was shorter than that of the control group [(23.5 ± 4.7) h] (t =1.937,P < 0.05) ; The hemoglobin level of the observation group was (116.7 ± 12.4) g/h after 72 hours treatment,and was higher than that of the control group [(91.5 ± 17.3) g/h] (t =2.013,P < 0.05) ;The pH of observation group was higher than that of control group after 12h,24h,48h and 72h treatment(all P < 0.05).Conclusion Octreotide combined with omeprazole is effective for upper gastrointesinal bleeding,and it can shorten the bleeding time and improve the cure rate.
7.Median effective dose of ropivacaine for spinal anesthesia when combined with sufentanil in patients undergoing caesarean section
Zhaohui CHEN ; Tai XIANG ; Qin GAO ; Yang LIU ; Junxiang LI ; Yong WAN ; Xiaolin YANG ; Bin CHEN
Chinese Journal of Anesthesiology 2011;31(5):583-585
Objective To determine the median effective dose (ED50 ) of ropivacaine for spinal anesthesia when combined with sufentanil in patients undergoing caesarean section. Methods Twenty-eight ASA Ⅰ or Ⅱ parturients, aged 18-40 yr, weighing 50-110 kg, undergoing cesarean section under combined spinal-epidural anesthesia, were enrolled in this study. Combined spinal-epidural anesthesia was performed at L2,3 interspace. The mixture of ropivacaine and 5 fig sufentanil was injected into the subarachnoid space over 30 s. The initial dose of ropivacaine was 11 mg. The dose was increased/decreased by 1 mg in the next patient. The ED50 and 95% confidence interval were calculated by up-and-down method. Results The ED50 of ropivacaine was 7.780 mg (95% confidence interval 6.850-8.836 mg). Conclusion When combined with sufentanil 5 μg, the ED50 of ropivacaine for spinal anesthesia is 7.780 mg in patients undergoing caesarean section.
8.Application of cytometric bead array in rapid high-throughput detection of small molecules.
Chang-bin XIAO ; Wei-jun KONG ; Qiu-tao LIU ; Mei-hua YANG ; Li WAN
China Journal of Chinese Materia Medica 2015;40(18):3515-3523
Cytometric bead array (CBA) is a new analytical technique, which can achieve real-time and rapid detection of targeted components in a small amount of sample. With many advantages of high throughput screening, high specificity and sensitivity, low cost, easy operation and good repeatability, this CBA technique has been widely used for the detection of various components in foods, agricultural products and environmental samples. Recently, it has got significant development in rapid detection of small molecules. This review briefly introduced the theory of CBA technique, summarized the application in the analysis of small molecules, such as mycotoxins, pesticide residues, shellfish toxins, and then prospected the application of trace small molecules detection in the complex matrices of traditional Chinese medicine and the development trend of it.
Drug Contamination
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High-Throughput Screening Assays
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instrumentation
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methods
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Immunoassay
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instrumentation
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methods
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Microspheres
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Pesticides
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analysis
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Toxins, Biological
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analysis
9.Comparison of diabetes(pre-diabetes) prevalence and awareness between province Shanxi and Fujian
Shaoyong XU ; Jie MING ; Bin GAO ; Yi WAN ; Chunbao YANG ; Gang CHEN ; Qiuhe JI
Chongqing Medicine 2013;(23):2765-2766,2768
Objective To compare diabetes(pre-diabetes) prevalence and awareness between Province Shanxi and Fujian .Meth-ods Study data was from China National Diabetes and Metabolic Disorders Study 2007-2008 .5 926 individuals(Shanxi 3 254 ,Fu-jian 2 672) were included as study participants .Standardized questionnaire was used to obtain demographic and lifestyle data .All participants were administered a glucose tolerance test .Results Overall diabetes prevalence was 9 .6% .The diabetes prevalence in Shanxi was lower compared with Fujian(8 .2% vs .11 .3% ,P<0 .001) .Overall pre-diabetes was 15 .4% and no significant differ-ence was found between the two provinces .The awareness of diabetes and pre-diabetes in Shanxi was lower compared with Fujian , although both provinces were not satisfactory .Conclusion Regional differences were showed in diabetes (pre-diabetes) prevalence and awareness between Province Shanxi and Fujian .
10.Study on transfection of adeno associated virus 2-ND4 gene into mitochondria
Shuo, YANG ; Lei, LIU ; Han, PEI ; Xing, WAN ; Duoduo, LU ; Weikun, HU ; Bin, LI
Chinese Journal of Experimental Ophthalmology 2014;32(8):693-695
Background Leber hereditary optic neuropathy (LHON) is mitochondrial DNA (mtDNA) disease and mainly leads to optical nerve degeneration.Its primary mechanism is synthesis disorder of DN4 protein due to variation of mtDNA 11778 locus.So to construct a vector with exogenous normal ND4 and transfect into mitochondria is a key of gene therapy for LHON.Objective This study was to investigate the in vitro transfection of adeno-associated virus (AAV)-ND4 gene into mitochondria.Methods Human renal epithelial cell lines transfected adenovirus E1A (293 cells) were regularly cultured and divided into two groups.Framework plasmids of recombinant AAV-ND4 or simple AAV2 were added to the cell medium respectively.The expression of ND4 in cells were located 12,24,36 and 48 hours after transfected by Y03 dual fluorescent quantum dots staining.The positive response for ND4 showed the green fluorescence.Results Cultured 293 cells grew well with 80% confluence.Abundant green fluorescence particles were seen in cytoplasm in the AAV-ND4 transfected group,but only red fluorescence from mitochondrial protein was seen in the simple AAV transfected group under the fluorescence microscope.Conclusions Exogenous ND4 protein can been successfully transfected into mitochondria using the ND4 gene constructed AAV.This result provides experimental evidence for the further study on gene therapy of LHON.