To study the compatible mechanisms and compatible proportion of Shaoyao Gancao decoction, the intestinal absorption of main ingredients in Shaoyao Gancao decoction SG11 (Baishao-Zhigancao 1: 1) , SG31 (Baishao-Zhigancao 3: 1), Baishao water decoction S and Zhigancao (G) were investigated and compared using in vitro everted intestinal sac model and in situ single pass intestinal perfusion (SPIP) model. The concentration of paeoniflorin (PF), liquiritin (LQ) and mono-ammonium glycyrrhizinate (GL) in test samples and samples of intestinal sac and intestinal perfusion was determined by HPLC. The intestinal absorptive amount and absorption parameters were calculated. Results showed that in the everted intestinal sac model, three ingredients could be absorbed by duodenum, jejunum and ileum, and the absorption in the jejunum was best for all 3 ingredients. The absorption rate of three ingredients in SG11 was significantly higher than that in single decoction (P < 0.05), but had no significant difference compared with SG31. In SPIP model, the absorption rate constant K(a), the apparent absorption coefficient P(app) and the absorption rate of three ingredients in SG11 were significantly higher than those in single decoction. Parameters of PF and GL in SG11 were significantly higher than those in SG31, but had no differences of LQ. It proved that the compatibility of Baishao and Zhigancao could improve the intestinal absorption of PF, LQ and GL. The absorption of each ingredient in SG11 was better than that in SG31.
Animals ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; pharmacokinetics ; Intestinal Absorption ; drug effects ; Intestines ; blood supply ; metabolism ; Male ; Rats ; Rats, Sprague-Dawley

Objective To study the effect of motor imagery therapy combined with conventional rehabilitation treatment on walking ability in patients with post-stroke hemiplegia.Methods Eighty patients with post-stroke hemiplegia were randomly divided into a treatment group (40 cases) and a control group (40 cases).All the patients in both groups were given basic medication and conventional rehabilitation treatment.In addition,the patients in treatment group were given motor imagery therapy.The 10 m maximum walking speed (10 m MWS),stride length,cadence,Fugl-Meyer motor assessment (lower limb) (FMA-L) and Holden's functional ambulation classification (FAC) were used to evaluate walking ability before the beginning of training and at the end of six weeks of training.Results Before intervention there was no significant difference between the two groups in terms of all the assessment (P ＞0.05).At the end of training,all measurements in both groups [the treatment group:10 m MWS (0.53 ± 0.20)m/s,stride length (78.91 ± 20.46) cm,cadence (78.10 ± 12.03) min,FMA-L (24.13 ±5.77),FAC (3.60±1.01);the control group:10 m M WS (0.42 ±0.15)m/s,stride length (69.75 ± 18.31)cm,cadence (71.14±9.29)/min,FMA-L (20.65 ±4.70),FAC (2.93 ±0.89)] were significantly better than those before training (P ＜ 0.05),and the improvements in treatment group were significantly better than those in control group (P ＜ 0.05).Conclusion Motor imagery therapy combined with conventional rehabilitation treatment can distinctly improve the walking ability in patients with post-stroke hemiplegia.

Objective: To observe the clinical efficacy of herb-partitioned spreading moxibustion at Baliao points plus climen for diminished ovarian reserve (DOR). Methods: A total of 60 patients with DOR were randomized into a spreading moxibustion group and a Western medicine group by the random number table method, with 30 cases in each group. The Western medicine group was treated with climen, starting from the 5th day of the menstrual cycle for 21 d. The spreading moxibustion group was treated with herb-partitioned spreading moxibustion at Baliao points on the basis of the medication in the Western medicine group, 1 h per time, once a week. The treatment was performed for 1 month as one treatment course in both groups, for 3 courses in total. The serum follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) in the patients were measured before and after treatment. The peak systolic velocity (PSV) and resistance index (RI) were also detected. The traditional Chinese medicine (TCM) symptom score was evaluated. The clinical efficacy was evaluated after treatment. Results: The total effective rate in the spreading moxibustion group was 93.3％, which was significantly higher than 80.0％ in the Western medicine group, and the difference between the groups was statistically significant (P<0.05). After treatment, the TCM symptom scores, the serum FSH levels, FSH/LH ratios and RI in both groups decreased, and the intra-group differences were all statistically significant (all P<0.05). The serum E2 level and PSV increased compared with those in the same group before treatment, and the intra-group differences were statistically significant (all P<0.05). After treatment, the TCM symptom score, the serum FSH level, FSH/LH ratio and RI in the spreading moxibustion group were lower than those in the Western medicine group, while the serum E2 level and PSV were higher than those in the Western medicine group, and the differences between the groups were statistically significant (all P<0.05). Conclusion: Herb-partitioned spreading moxibustion at Baliao points plus climen can produce valid therapeutic efficacy for DOR. It can improve the clinical symptoms, regulate serum hormone levels and increase ovarian blood perfusion, thus improving ovarian reserve function, producing more significant efficacy than climen alone.

Aim: To explore the apoptosis and mechanisms of human gastric cancer cells MGC-803 induced by RAA-11. Methods: MTT and colony assays were used to detect the survival of MGC-803 cells treated with RAA-11. The effect of RAA-11 on the apoptotic morphology of MGC-803 cells was observed by Hoechst 33258 staining. The effect of RAA-11 on the apoptosis rate of MGC-803 cells was detected with flow cytometry. The effects of RAA-11 on apoptosis related protein expression of caspase-3, Bcl-2 and Bax as well as pathway proteins ERK and p-ERK in MGC-803 cells were observed by Western blot. Results: MTT assay showed that the proliferation of MGC-803 cells was effectively inhibited in a time- and concentration-dependent manner (P <0. 01). The result of colony suggested that RAA-11 could inhibit the proliferation of MGC-803 cells. Hoechst 33258 staining indicated that the nucleus of MGC-803 cells had a typical apoptotic morphological change after intervention with RAA-11. The result of flow cytometry suggested that RAA-11 had a significant apoptotic effect on MGC-803 cells. The expressions of Bax and caspase-3 were significantly up-regulated (P <0. 01), and the expressions of Bcl-2, ERK and p-ERK were down-regulated (P <0. 01) in MGC- 803 cells treated with RAA-11. Conclusions: RAA-11 inhibits the proliferation and induces apoptosis in MGC-803 cells, which may be related to the inhibition of ERK/MAPK pathway.

Aim: To explore the effects of RAA-11 on the growth and migration of human gastric cancer cells MGC-803 via the EGFR-JNK pathway. Methods: The effect of RAA-11 on the activity of human gastric mucosa cells GES-1 and human gastric cancer cells MGC- 803 was observed by MTT assay. The effect of RAA-11 on the migration in human gastric cancer MGC-803 cells was detected by scratch test. The effect of RAA- 11 on EGFR mRNA expression in human gastric cancer MGC-803 cells was detected by qRT-PCR. The changes of apoptosis-related proteins caspase-3, Bcl-2, Bax as well as pathway proteins EGFR, JNK, and p-JNK expression in human gastric cancer MGC-803 cells were assessed by Western blot. Results: MTT results indicated that RAA-11 significantly inhibited the proliferation of MGC-803 cells in human gastric cancer compared with human gastric mucosa GES-1 cells (P < 0. 01), suggesting that RAA-11 had a selective effect on MGC-803 cells. The scratch result suggested that RAA-11 could suppress the migration of MGC-803 cells. The results of qRT-PCR indicated that RAA-11 decreased the expression level of EGFR mRNA in human gastric cancer MGC-803 cells. Western blot results suggested that RAA-11 up-regulated apoptosisrelated proteins caspase-3 and Bax in human gastric cancer MGC-803 cells, promoted the expression of pathway proteins JNK and p-JNK (P < 0. 01), downregulated the expression of apoptosis-related proteins Bcl-2, and reduced the expression level of pathway protein EGFR (P < 0. 01). Conclusions: RAA-11 can inhibit the proliferation and migration and induce apoptosis of human gastric cancer MGC-803 cells by the EGFR-JNK pathway.

Objective:To explore the correlation between gene mutations and clinical characteristics,prognosis of myelodysplastic syndromes(MDS).Methods:Clinical data of 131 patients with MDS were collected from the First Hospital of Lanzhou University from June 2015 to February 2023,which 19 of them developed into secondary acute myeloid leukemia(sAML)during follow-up time.Second generation sequencing technology was used to detect the mutation types of MDS disease-related genes,drawn gene maps,and analyzed their correlation and prognosis based on the clinical data of patients.Results:The median age of 131 MDS patients was 58(17-86)years old.The ratio of male to female was 1.3:1.A total of 148 gene mutations and 25 types were found in the center.U2AF1 and ASXL1 were often co-mutations with other genes,which were accompanied by 20q-and normal karyotype(NK)respectively.SETBP1 and SRSF2 were more common in patients over 60 years old,while NPM1 and WT1 under 60 years.Older patients had a higher the number of genetic mutations than younger patients.The incidence of SF3B1 and RUNX1 in males was higher than females and DNMT3A in females was higher than males.The number of gene mutations in sAML was higher than MDS(1.8 vs 1.0,P=0.006).The univariate and multivariate analysis showed that IPSS-R prognostic score≥ 3.5,TP53 were adverse factors for poor prognosis in MDS patients.Patients with monoallelic mutation(ma-TP53)and wild-type(wt-TP53)TP53 had OS better than biallelic mutation(bi-TP53)(P=0.003).The OS of MDS patients was better than sAML(P=0.01)and transplant patients was significantly better than non-transplant patients(P=0.036).Conclusion:Gene mutation is closely related to cytogenetic indexes and clinical features(peripheral blood cell count,sex,age).IPSS-R prognostic score and TP53 were risk factors affecting OS in MDS patients.

Objective To study the feasibility of the therapy for the preoperative preparation maternal anxiety with patient-controlled transcutaneous electrical stimulation on auricular Shenmen point in cesarean section.Methods Totals of 60 primiparas (SAS score ＞ 30)who underwent elective cesarean section were randomly divided into experiment group ( n =30 ) and control group ( n =30 ).Experiment group received patient-controlled sedation with transcutaneous electrical stimulation on auricular point after enter into the operating room (took Shenmen point frequency 1.5 Hz).And the strength was controlled by themselves,continued stimulation for 30 minutes.While control group didn' t receive electrical stimulation.The indexes were observed such as HR,MAP,fetal heart rate when the women came to the operating room (T0 ),after receiving 30 minutes stimulation (T1),and the VAS score and the concentration of plasma angiotensin Ⅱ was compared.Results HR,MAP,VAS score and the concentration of plasma angiotensin Ⅱ of experiment group were (72 ± 9 ) bpm,( 84 ± 11) mm Hg,(49.8 ± 20.8 ),and (73.9 ± 21.8 ) ng/L,respectively,which was lower than that of the control group,and the difference was statistically significant (t =2.143,2.648,3.576,2.163 ; P ＜0.05).There was no significant difference found in the fetal heart rate between two groups ( P ＞ 0.05 ).Conclusions Patient-controlled transcutaneous electrical stimulation on auricular Shenmen point can keep calm,decrease MAP and restrain the stress response of maternal in cesarean section for preoperative preparation.

OBJECTIVETo explore the changes in the expressions of glial fibrillary acidic protein (GFAP) and growth- associated protein-43 (GAP-43) in retinal ganglial cells after neural transplantation.

METHODSThirty-nine rats were randomized into normal control group, nerve amputation group and nerve amputation with peripheral nerve transplantation group. Immunohistochemistry was used to detect the changes in the expressions of GFAP and GAP-43 at different time points after the operations, and real-time PCR was employed to detect the mRNA expressions of 13 genes in the retinal ganglial cells of the rats.

RESULTSImmunohistochemistry showed obviously increased GFAP expressions in the retina following the nerve amputation. GFAP expression was down-regulated while GAP-43 expression upregulated in the retinal ganglial cells after peripheral nerve transplantation. Real-time PCR results showed that 5 days after the operations, retinal GFAP and GAP-43 expressions increased significantly in the nerve amputation group and peripheral nerve transplantation groups as compared with those in the control group, but GAP-43 expression decreased significantly in the former two groups afterwards.

CONCLUSIONThe regenerated retina may adjust the production of GFAP. The retinal ganglial cells express GAP-43 during retinal regeneration. Up-regulation of the expression of GAP-43 provides the evidence for nerve regeneration following the nerve transplantation.

Animals ; Axons ; Female ; GAP-43 Protein ; genetics ; metabolism ; Glial Fibrillary Acidic Protein ; genetics ; metabolism ; Nerve Regeneration ; genetics ; Optic Nerve ; transplantation ; Optic Nerve Injuries ; metabolism ; Random Allocation ; Rats ; Retinal Ganglion Cells ; metabolism

OBJECTIVETo explore the effect of kallikrein-binding protein (KPB) in protecting retinal ganglion cells (RGCs) and promoting axonal regeneration following optical nerve injury in rats.

METHODSCrush injury of the optic nerve at 0.5-1.0 mm from the eyeball was induced in rats, which received subsequent KBP injection into the vitreous cavity (experimental group) and PBS injection (control group). At 7, 14 and 21 days after the injury, the rats were sacrificed and frozen sections of the eyeball were prepared to observe the structure and thickness of the retina and count the number of survival RGCs with HE staining. The optic nerves were collected for Western blotting to assess the effect of KBP on the RGCs and axonal regeneration.

RESULTSRGC counts and retinal thickness showed significant differences between the two groups. Western blotting also demonstrated a significant difference in the expression of the nerve regeneration marker protein GAP-43 between the two groups.

CONCLUSIONKBP offers protection on RGCs and promotes regeneration of the optic nerve axons after optic nerve injury in rats.

Animals ; Axons ; physiology ; Female ; GAP-43 Protein ; metabolism ; Nerve Regeneration ; drug effects ; physiology ; Neuroprotective Agents ; pharmacology ; Optic Nerve Injuries ; drug therapy ; Rats ; Rats, Sprague-Dawley ; Retinal Ganglion Cells ; drug effects ; physiology ; Serpins ; pharmacology

Flavonol synthase (FLS) is one of the key enzymes in flavonoids metabolic pathways. In this study, middle sequence was obtained from Carthamus tinctorius transcriptome sequencing results. Full-length cDNAs of FLS was cloned from petals of C. tinctorius to FLS by using RT-PCR and RACE technology. Its full-length cDNA was 1,201 bp, with an open reading frame of 1,101 bp and 336 encoded amino acids. The phylogenetic analysis showed that, FLS gene encoded amino acids in C. tinctorius were highly homologous with amino acids in congeneric Compositae species, especially Rudbeckia laciniata. The pBASTA-FLS plant expression vector was successfully built by the molecular biology method, which lays a foundation for further studying biology functions of the gene and biosynthesis mechanism of flavonoids.
Amino Acid Sequence ; Base Sequence ; Carthamus tinctorius ; classification ; enzymology ; genetics ; Cloning, Molecular ; DNA, Complementary ; genetics ; metabolism ; Molecular Sequence Data ; Open Reading Frames ; Oxidoreductases ; genetics ; metabolism ; Phylogeny ; Plant Proteins ; genetics ; metabolism