Objective To investigate the effects of dexmedetomidine on tonsillectomy in children with sevoflurane combined with anesthesia recovery period of restless.Methods A total of 62 patients with tonsillectomy under general anesthesia from 2011 to 2015 in our hospital werecollected and randomly divided into the control group and the experiment group with 31 cases in each.Two groups of children were treated by 7％sevoflurane inhalation anesthesia induction,supplemented by a small amount of intravenous anesthetics propofol 2 mg/kg,sufentanil 2 μg/kg,CIS atracurium 150 μg/kg intravenous injection.Patients in the experiment group were treated by dexmedetomidine 0.2 μg/(kg·h)continuous intravenous infusion until the end of the operation,patients in the control group were treated by normal saline intravenous infusion.Compared the systolic blood pressure and heart rate of the 2 groups of before anesthesia induction,after anesthesia induction,after operation 5min,the end of the operation ; occurred rate of restless at after anesthesia extubation 5minutes,10minutes,15minutes,20minutes,25minutes.Results Compared with the control group,the systolic blood pressure and heart rate of the experiment group without statistical significance before anesthesia induction; after anesthesia induction,after operation,5min,at the end of operation,the systolic blood pressure and heart rate were lower(P<0.05); children recovery time,extubation time,recovery time of delivery from the anesthesia room were shorter(P<0.05); occurred rate of restless were lower after anesthesia extubation 5minutes,10minutes,15minutes,20minutes,25minutes(P<0.05).Conclusion Dexmedetomidine can prevent tonsillectomy after sevoflurane anesthesia result in the recovery period of restless.

Objective:To study the toxicity of 11 dehydrocorticosterone on hippocampal neurons and to determine whether 11? hydroxysteroid dehydrogenase (11? HSD1) is involved in the neurotoxity. Methods:Western blotting, radiometric enzyme activity assay and MTT assay were employed in this study. Results:Both 11? HSD1 protein and bioactivity were positive in the hippocampal neurons as demonstrated by Western blotting and radiometric enzyme activity assay. At concentration of 10 -6 mol/L, 11 dehydrocorticosterone was neurontoxic to hippocampal neurons cultured in serum free DMEM medium. This neurotoxic effect of 11 dehydrocorticosterone was blocked by 11? HSD1 inhibitor carbenoxolone (CBX) and glucocorticoid receptor (GR) antagonist RU38486, but not by mineralcocorticoid receptor (MR) antagonist spironolatone. Corticosterone and its derivative 11 dehydrocorticosterone up regulated 11? HSD1 level. Conclusion:11 dehydrocorticosterone has toxicity on hippocampal neurons, and it can be blocked by CBX, suggesting 11? HSD1 may convert biologically inactive 11 dehydrocorticosterone to active corticosterone. The up regulation of 11? HSD1 by glucocorticoids in return exaggerates the neurotoxic effect of corticosterone, which may play a positive role in the delayed neuron death during stress.

Adolescent ; Adult ; Atropine ; therapeutic use ; Female ; Foodborne Diseases ; drug therapy ; Humans ; Insecticides ; poisoning ; Male ; Muscarinic Antagonists ; therapeutic use ; Organophosphorus Compounds ; Pesticide Residues ; poisoning ; Pesticides ; poisoning ; Treatment Outcome

Adult ; Humans ; Male ; Neck Injuries ; complications ; Pharynx ; injuries ; Trachea ; injuries

Objective To evaluate the clinical application of Nutritional Risk Screening 2002 (NRS 2002) in inpatients.Methods Totally 400 inpatients who were admitted to Tianjin Tianhe Hospital from Novem- ber 2008 to March 2009 were enrolled in this study.Physical examinations,including body height and body meas-urement,were performed the next morning after admission.The nutritional status was evaluated with NRS 2002.Results In all 400 inpatients.NRS 2002 was strongly practicable in 306 patients (76.5%) and weakly practica-ble in 94 patients (23.5%);Ninety-six patients (24.0%) had nutritional risks,which were most common in the department of internal medicine and the Department of neurology.The average age of patients with nutritional risks was (79.0±11.4) years,which was significantly higher than that of patients without nutritional risks [(58.1±15.8) years] (P<0.01).Conclusion NRS 2002 is effective and practicable in evaluating the nutritional status of inpatient.

Objective To investigate the prevalence of abnormal glucose regulation (AGR) in patients with coronary artery dis-ease (CAD) in the department of cardiology of Tianjin Medical University General Hospital. Methods Sixty seven patients with CAD in the department of cardiology of Tianjin Medical University General Hospital were consecutively recruited from Jun 1, 2005 to Aug 31, 2005. Patients were asked to perform oral glucose tolerance test (OGTT) to characterize the glucose regulation status except for those with previously known diabetes mellitus (DM). Results Most of patients with CAD in the study had abnormal glucose regulation. The prevalence of AGR was 88.1%. The prevalence of DM and IGT were 56.7% and 31.3%, respectively. Without OGTT, 42.5% DM and 48% IGR and 85% AGR would remain undiagnosed by fasting plasma glucose (FPG) detection alone. Conclusion A majori-ty of patients with CAD in the study have AGR and OGTT is needed to promptly and accurately disclose these patients.

Objective: To investigate the proliferation of three subtypes of odontogenic keratocysts(OKC), namely simple(not recurrent),recurrent and basal cell naevus syndrome(BCNS) associated lesions. Methods: Ki 67 expression was studied in 34 odontogenic keratocysts(simple, n =10;recurrent, n =12;syndrome, n =12)by biotin streptavidin method using Ki 67 monoclonal antibody after microwave treatment. Ki 67 positive cells were counted manually and related to the area of epithelial lining as determined by computer image analyzer. Results: Ki 67 positive cells per mm 2 in simple odontogenic keratocysts ①, recurrent odontogenic keratocysts ② and BCNS ③ were 1 812.29?606.47, 2 393.88 ?997.08 and 3 983.17?858.92 respectively(① or ② vs ③ P 0.05). Conclusion: The basal cell naevus syndrome associated OKC has a higher rate of epithelial proliferation than non syndrome OKC, the increased epithelial proliferation is correlated with increased recurrence potential. Ki 67 may be an alternative method to differentiate syndrome and non syndrome OKC.

Objective To address angiotensin Ⅱ type 1 receptor (AT 1R) gene polymorphism and its relationship with plasma endothelin (ET) in PIH. Methods Gene polymorphism of AT 1R at site 1166 was detected with polymerase chain reaction (PCR), digestion of restriction enzyme and electrophoresis. Plasma ET was measured by radioimmunoassay. Results 1. The frequencies of genotype(AC and CC) at 1166 of AT 1R gene and C allele in PIH individuals were significantly higher than those of control group, (? 2=11.06, 9.73, respectively, P0.05). Conclusions 1. The variant (A→C) of 1166 polymorphism site of AT 1R is associated with the generation of PIH, and C allele may be a susceptible gene to PIH. 2.There is relationship between AT 1R gene and plasma ET level in PIH patients.

Objective:To investigate the factors related to the occurrence of submacular fluid (SMF) after pars plana vitrectomy (PPV) in the treatment of rhegmatogenous retinal detachment (RRD).Methods:A retrospective case series observational study was performed.A total of 103 patients (103 eyes) with RRD who underwent 23G vitrectomy combined with silicone oil tamponade in The First Affiliated Hospital of Zhengzhou University from May 2017 to August 2019 were included.There were 55 males and 48 females, aged from 17 to 77, with an average of (48.65±15.80) years.The macular morphology was observed by optical coherence tomography (OCT) before surgery and 1 month and 3 months after surgery.The patients were divided into SMF group and no SMF group according to OCT results at 1 month after surgery.Clinical data were compared and analyzed, including age, gender, duration of symptoms, axial length, the preoperative status of macula (on or off), retinal detachment caused by inferior retinal break or not, the way to drain subretinal fluid intraoperative, best corrected visual acuity (BCVA). FFA was performed in 3 eyes and microperimeter examination in 2 eyes with SMF according to patients' willingness.A multivariate binary Logistic regression was used to analyze the risk factors of SMF after vitrectomy in RRD patients.This study adhered to the Declaration of Helsinki and was approved by the Ethics Committee of The First Affiliated Hospital of Zhengzhou University (No.2020-KY-334). Written informed consent was obtained from each subject prior to surgery.Results:Postoperative optical fundus examination and ocular ultrasound indicated that retinas of all eyes were completely anatomically reattached.There were 10.7% (11/103) of eyes developed SMF.The microperimeter examination results of 2 patients showed that their visual functions were worse at 3 months than those at 1 month after operation.The BCVA of patients with SMF at 1 month and 3 months after operation were worse than those without SMF, and the differences were statistically significant (both at P<0.05). Logistic regression analysis showed that macula-off RD before surgery and RD caused by inferior break of retina were risk factors for the occurrence of SMF (OR=6.401, P=0.041; OR=19.819, P=0.005), while the increasing age and the drainage of subretinal fluid through draining hole were protective factors (OR=0.939, P=0.016; OR=0.123, P=0.040).Conclusions:Macula-off RD before surgery, and RD caused by inferior break of retina may be the risk factors of SMF after PPV combined with silicone oil tamponade for RRD.Nevertheless, the increasing age and the drainage of subretinal fluid through draining hole may decrease the occurrence of SMF.And SMF after PPV may delay visual recovery.

Objective To compare the difference of effect between principal component regression analysis and projection pursuit regression analysis when collinearity exists in data.Methods Evaluating the advantages and disadvantages of the two modeling methods by using the actual data on two aspects:the fitting effect and the predicting effect.Results The principal component regression model showed that the coefficient of determination was 0.8172,the mean of absolute relative error was 6.42％ and the mean square of prediction error was 0.61.The projection pursuit regression model,on the other hand,showed that the coefficient of determination ranged from 0.8851 to 0.9944,the mean of absolute relative error ranged from1.11％ to 4.81％ and the mean square of prediction error ranged from 0.03 to 0.38.Conclusion The analysis results based on the actual data with collinearity indicate that the projection pursuit regression analysis outperforms the principal component regression analysis both in fitting and predicting effect.