1. Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines
Latifah Saiful YAZAN ; Napsiah ABD RAHMAN ; Kim Wei CHAN ; Wan Nor Hafiza WAN ABD GHANI ; Yin Sim TOR ; Jhi Biau FOO ; Latifah Saiful YAZAN ; Napsiah ABD RAHMAN ; Wan Nor Hafiza WAN ABD GHANI ; Yin Sim TOR ; Jhi Biau FOO
Asian Pacific Journal of Tropical Biomedicine 2016;6(5):404-409
Objectives: To determine the cytotoxicity of crude ethanolic extract, n-butanol fraction and aqueous fraction on selected cancer cell lines, and to observe the morphological changes of the cancer cells treated with n-butanol fraction. Methods: The cytotoxic effect of n-butanol fraction, crude ethanolic extract and aqueous fraction on breast cancer (MCF-7 and MDA-MB-231), colon cancer (HT29), lung cancer (A549), cervical cancer (HeLa) and normal mouse fibroblast (3T3) cell lines was determined using MTT assay. The morphological changes of the treated cells were observed under an inverted light microscope. Results: n-Butanol fraction was the most cytotoxic towards HT29 and MCF-7 cells in a dose-dependent manner compared to crude ethanolic extract and aqueous fraction (P < 0.05). The IC
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