1.Expression of p53 and nm23-H1 in nasopharyngeal carcinoma and their significance
Kang LI ; Zhimao ZHANG ; Minjie WAN
Cancer Research and Clinic 2014;26(1):52-55
Objective To sdudy the expression of p53 and nm23-H1 proteins and their clinical significance in nasopharyngeal carcinoma.Methods The expression of p53 and nm23-H1 proteins were detected by immunohistochemistry method in 40 cases with nasopharyngeal carcinoma and 22 cases with chronic inflammation of nasopharyngeal mucosa tissues.Results Positive rates of p53 and nm23-H1 in chronic nasopharyngitis group were 1.0 %,27.2 %,and in the NPC group were 92.5 %,55.0 %.There were 9 cases with the positive expression of p53 in 22 cases of nasopharyngeal carcinoma tissues with the positive expression of nm23-H1 (40.9 %).There were 17 cases with the positive expression of p53 in 18 cases with the negative expression of nm23-H1 (94.4 %).The expression of p53 and nm23-H1 proteins in nasopharyngeal carcinoma were much higher than that in chronic inflammation of nasopharyngeal mucosa tissues.The expression of p53 protein in nasopharyngeal carcinoma with metastatic lymph node was higher than that in nasopharyngeal carcinoma without metastatic lymph node,but nm23-H1 protein lower.The expression of p53 protein was positively correlated with the metastasis,clinnical staging and pathological classification but not correlated with T classification of nasopharyngeal carcinoma.The expression of nm23-H1 protein was negative correlation with the metastasis and clinical staging of nasopharyngeal carcinoma.Conclusion p53 and nm23-H1 play important coordinated regulation roles in the carcinogenesis,development and metastasis of nasopharyngeal carcinoma and will probably become the key biological marks in the judging and evaluating prognosis of nasopharyngeal carcinoma.
2.Determination of methoxychlor in rabbit serum and its metabolic products with high performance liquid chromatography.
Xiao-yun ZHANG ; Wan-shu LI ; Li-kang ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(8):622-624
Animals
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Chromatography, High Pressure Liquid
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methods
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Methoxychlor
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blood
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metabolism
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Phenols
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blood
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Rabbits
3.Influence of psychological persuasion and drug intervention on anxiety and depression in patients with myocardial bridge
Wentang NIU ; Pan WANG ; Kang WAN ; Jian LI
Chinese Journal of cardiovascular Rehabilitation Medicine 2015;24(5):485-488
Objective:To explore influence of psychological persuasion and drug intervention on anxiety and depres‐sion in patients with myocardial bridge .Methods:A total of 126 patients ,who were confirmed as had myocardial bridge by coronary angiography (CAG) ,were divided into routine treatment group (n=62) and psychological intervention group (n=64 ,received psychological persuasion ,flupentixol melitracen tablet and sertraline therapy based on routine treatment ) . Another 40 cases with normal CAG results were enrolled as normal control group ,three groups received anxiety and depres‐sion assessment using Zung self‐rating anxiety scale (SAS) and self‐rating depression scale (SDS) before and after interven‐tion ,and the results were compared among three groups .Results:Before intervention ,there were no significant difference in SAS and SDS standardized scores between routine treatment group and psychological intervention group (P>0.05) ,but they were significantly higher than those of normal control group [SAS:(50.38 ± 7.10) scores ,(49.78 ± 7.42) scores vs . (25.70 ± 3.80) scores ,SDS:(48.5 ± 7.43) scores ,(47.67 ± 8.12) scores vs .(26.47 ± 4.10) scores , P<0.01 all] .After intervention ,SAS and SDS standardized scores of psychological intervention group significantly reduced compared with be‐fore intervention (P<0.01) ,and they were significantly lower than those of routine treatment group [SAS:(40.84 ± 6.23) scores vs .(50.88 ± 8.63) scores ,SDS:(39.82 ± 5.84) scores vs .(50.69 ± 8.48) scores] ,P<0.01 all .Conclusion:Psychological persuasion combined drug intervention could significantly reduce or relieve anxiety and depression in patients with myocardial bridge .
5.Dynamic predictive modeling of extraction process for red ginseng using near-infrared spectroscopy.
Jie-Qiang ZHU ; Wan-Fang PAN ; Yi ZHONG ; Xiao-Hui FAN ; Li-Yuan KANG ; Zheng LI
China Journal of Chinese Materia Medica 2014;39(14):2660-2664
It is the objective of this study to develop dynamic predictive model for the extraction process of red Ginseng using NIR spectroscopy. NIR spectroscopy was collected online and PLSR models were developed for total quantity of ginsenosides. The performance of NIR prediction model achieved R, RMSEC, RMSEP of 0.996 09, 0.018 9, 0.016 8, respectively. A first order dynamic mass transfer model was combined with NIR prediction of the quality indicator to predict the trajectory of the extraction process based upon the initial 3 or 4 data points. The results showed good agreement with actual measurements indicating reasonable accuracy of the predictive model. It could potentially be used for advanced predictive control of the extraction process.
Chemical Fractionation
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methods
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Ginsenosides
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chemistry
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isolation & purification
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Models, Theoretical
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Panax
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chemistry
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Spectroscopy, Near-Infrared
6.Expressions of transient receptor potential A1 and related inflammatory factors in the rat model of prostatic inflammation.
Bao-xing HUANG ; Wan-li CAO ; Xin HUANG ; Jun DAI ; Heng-chuan SU ; Kang CHENG ; Fu-kang SUN
National Journal of Andrology 2015;21(1):23-30
OBJECTIVETo explore the molecular mechanism of pain associated with chronic prostatitis and chronic pelvic pain syndrome (CP/CPPS) in the rat model of prostatic inflammation.
METHODSThirty-six male SD rats were equally randomized to an experimental and a control group, the former injected with 50 μl of 3% λ-carrageenan into the ventral prostate to make the model of non-bacterial prostatic inflammation, while the latter with the same volume of sterile saline solution. At 1, 2 and 4 weeks after modeling, the prostate, L6-S1 dorsal root ganglion (DRG) and spinal cord were harvested for examination of the expressions of the nerve growth factor (NGF), transient receptor potential ankyrin 1 (TRPA1), and calcitonin-gene-related peptide (CGRP) by immunohistochemistry and Western blot.
RESULTSThe expressions of NGF, TRPA1 and CGRP in the prostatic tissue were all significantly increased in the experimental group as compared with the control (P <0.05), with a gradual decrease with the prolonging of time (P <0.05). In the L6-S1 DRG and spinal cord, the expressions of NGF, TRPA1 and CGRP exhibited no significant differences between the experimental and control groups at 1 week after modeling (P >0.05) and kept at high levels in the experimental group at 2 and 4 weeks, though not significantly different from those at 1 week (P >0.05). Statistically significant differences were observed in the expressions of the three proteins in the experimental rats among different time points (P <0.05), but not between the two groups at any time point (P >0.05).
CONCLUSIONThe molecular mechanism of CP/CPPS can be evaluated in the rat model of prostatic inflammation established by injecting λ-carrageenan into the prostate. TRPA1 may play an important role in connecting the upstream and down-stream pathways of CP/CPPS-associated pain.
Animals ; Calcitonin Gene-Related Peptide ; metabolism ; Carrageenan ; Chronic Disease ; Chronic Pain ; metabolism ; Ganglia, Spinal ; metabolism ; Humans ; Male ; Nerve Growth Factor ; metabolism ; Pelvic Pain ; metabolism ; Prostatitis ; chemically induced ; metabolism ; Rats ; Rats, Sprague-Dawley ; Spinal Cord ; metabolism ; TRPA1 Cation Channel ; TRPC Cation Channels ; metabolism
7.Influence of aberrant behaviors on the mental health of caregivers for patients with pervasive developmen-tal disorders
Mengmeng DU ; Chuanyuan KANG ; Xuerong LI ; Shuai WAN ; Yang CAO ; Kaijing DING ; Runxu YANG
Chinese Journal of Nervous and Mental Diseases 2015;(4):199-202
Objective To investigate the influence of hyperactivity, Stereotyped behaviors , self-injury and irritabili?ty on parenting stress and emotions of the caregivers for patients with pervasive developmental disorders (PDDs). Methods Abnormal behaviors during last month were assessed in 138 PDDs patients by using Aberrant Behavior Checklist (ABC) and Conners Index of Hyperactivity (CIH). The parenting stress and the emotional state of the patients’parents were evalu?ated by the Caregiver Strain Questionnaire (CGSQ) and Hospital Anxiety and Depression Scale (HAD), respectively. Re?sults Anxiety, depression and combination of anxiety and depression were present in 6.2%, 17.8%and 29.5%of PDDs caregivers, respectively. The partial correlation analysis showed that CGSQ score of patients’parents positively correlated with CIH score (r=0.201, P=0.023) and stereotyped act (r=0.189, P=0.033) of the patients, and negatively correlated with stereotyped speech (r=-0.219, P=0.013). The anxious mood of parents positively correlated with stereotyped act (r=0.206, P=0.021). Conclusions Anxiety and depression are common in caregivers of PDDs. Except for stereotyped speech, aber?rant behaviors of the PDDs increase parenting stress and worsen anxious mood of caregivers.
8.Comparative study between single dose dexmedetomidine and fentanyl in the management of emergence agitation in adult patients after general anesthesia
Kang WAN ; Lei-bo ZHANG ; Min-min ZHU ; Wen-jing LI
The Journal of Clinical Anesthesiology 2014;(12):1218-1220
Objective To investigate the effects of single dose of dexmedetomidine and fentynyl on treatment of emergence agitation in adult patients after general anesthesia.Methods Sixty patients suffering from emergence agitation after general anesthesia were divided into two groups randomly(n=30 each):dexmedetomidine group (group D)and fentaynl group (group F).Patients in group D received dexmedetomidine at a dose of 0.5μg/kg,and those in group F received fentanyl at a dose of 1μg/kg respectively.If patients still agitate after above treatment,intermission infusion of propofol at bolus of 1.0 mg/kg was used for further treatment till patients attain peaceful state.Results Twen-ty-nine paients in group D had no agitation,only one patient had mild agitation after administration of dexmedetomidine,while four patients had severe agitation,four patients had moderate agitation and 9 patients had mild agitation after administration of fentanyl(P<0.05);the time from first treatment to no agitation in group D was (11±3)min,shorter than that in group F (27±9)min (P<0.05);the dose of propofol used in group D was (50±0)mg,less than accumulative doses of propofol in group F (270±40)mg (P<0.05);21 (70%)patients in group D had radycardia and 9 (30%)patients in group F had respiratory depression(P<0.05).Conclusion Intravenous dexmedetomidine at a dose of 0.5 μg/kg attainted was effective in treating emergence agitation in adult patients after general anes-thesia as compared with fentanyl.
9.The relationship between nerve fiber bundle and muscle strength recovery in patients with acute ischemic stroke observed by magnetic resonance diffusion tensor imaging
Minglei CHEN ; Chaoming HE ; Kang LIN ; Mingwu PANG ; Jiangjun QIN ; Xiangxin WAN ; Zhiwei LI
Chongqing Medicine 2017;46(23):3203-3205
Objective To investigate the correlation between FA value,ADC value and limb muscle strength score measured by magnetic resonance imaging in patients with ischemic stroke,aims to to analyze the clinical value of magnetic resonance imaging in limb muscle strength.Methods Twenty patients with acute cerebral infarction and treated from June 2015 to Junly 2016 were recruited from This hospital,and the simplified Fugl-Meyer motor function score was observed for all patients within 3 days.Tensor imaging examination was conducted to observe the distribution of nerve fiber bundles,FA value,ADC value changes.Results The FA value and ADC value of the infarct side were significantly different from those of the contralateral side(t=8.70,t=-18.70,P<0.05);There were significant differences in FA value and ADC value between the infarcted ventricle hind limbs and the contralateral side of the infarcted ventricle(t=-5.16,t=-5.08,P<0.05).The FA value of the infarcted ventral hind limbs had positive correlation with the simplified Fugl-Meyer motor function score(R=0.863,P=0.013).Conclusion FA value and ADC value of acute infarct and internal hindlimb are lower than FA value and ADC value of contralateral normal white matter.The FA value of internal capsule hind limbs is closely related to the simplified Fugl-Meyer motor function score.
10.Incidence of aberrant behaviors in children with pervasive developmental disorders
Mengmeng DU ; Chuanyuan KANG ; Xuerong LI ; Shuai WAN ; Yang CAO ; Yan ZHANG ; Runxu YANG
Chinese Journal of Behavioral Medicine and Brain Science 2015;24(11):1020-1022
Objective To evaluate the incidence of four aberrant behaviors in patients with pervasive developmental disorders (PDDs).Methods The abnormal behaviors during the last month in 138 PDDs patients were assessed with Aberrant Behavior Checklist and Conners Index of Hyperactivity.Results The incidence of aberrant behaviors was 61.6% for hyperactivity,81.9% for stereotyped behaviors,13.77% for self-injury,and 58.70% for irritability in PDDs patients.The occurance of irritability in autism patients was higher than that of asperger syndrome (AS) patients (x2 =5.623,P=0.018).Conclusion Stereotyped behaviors,hyperactivity and irritability are common in children with PDDs.Autism patients are more likely to exhibit irritability behaviors than AS patients.