1.Circumferential mucosectomy for the treatment of prolapsed hemorrhoids
Chuangang FU ; Wei ZHANG ; Hantao WAN
Chinese Journal of General Surgery 1993;0(02):-
Objective To evaluate circumferential mucosectomy for the treatment of prolapsed hemorrhoids. Methods Ten patients with Ⅲ or Ⅳ degree of circular prolapsed hemorrhoids were operated on with circumferential mucosectomy using circular stapler. The mean age was 65 years old with hemorrhoids history of 3~25 years. Results Hemorrhoids in 8 of the 10 patients disappeared immediately after operation and 2 disappeared on the second day postoperatively. 3 patients complained local pain and 6 patients suffered from temporary urine retention after operation. No significant edema or bleeding ensued. The mean postoperative hospital stay was 4 days. No recurrence developed at an averaged 2 months′ follow up. Conclusion The new technique provides a significantly less painful choice of treatment for severely prolapsed circular hemorrhoids. However, the long-term outcome remains to be identified.
2.English Movies in Teaching College English
Lei WAN ; Jue FU ; Xinjun ZHANG
Chinese Journal of Medical Education Research 2005;0(06):-
The present paper is a study on the use of English movies in the teaching of college English,focusing on the design and interactive teaching process of the course "The Appreciation of English Movies".The paper argues that English movies play a positive role in creating an authentic communicative situation and acquainting students with western culture.
4.Study of the Expression of the Phospholipase A_2,Interleukin-1? and Tissue Inhibitor of Matrix Metalloproteinase-1 Following Traumatic Brain Injury
Tingfu LI ; Lihua WAN ; Wei ZHANG ; Dengjun FU
Journal of Medical Research 2006;0(03):-
Objective To investigate the expression of the cytosolic phospholipase A2(c-PLA2),interleukin-1 beta(IL-1?),tissue inhibitor of matrix metalloproteinase-1(TMP-1) in rat brain after injury.Methods The model of traumatic brain injury originally described by Feeney was employed.The mRNA was analyzed by RT-PCR.Results The mRNA of cytosolic phospholipase A2 was increased at 2 hour and its peak was at 7 day.At 14 day,the level of cytosolic phosphalipase A2 mRNA was still in high level as compared to the control group.Likely,the enhancement expression of interleukin-1? was seen at the 1 hour and the peak time was from 5 to 8 hour.At 72 hour,it decreased to normal level.The expression of inhibitor of matrix metalloproteinase-1 was increased at the 2 hour,and the highest expression level was seen during 48 to72 hour.It came down to normal level at 14 day after injury.Conclusion The augment of the expression of the cytosolic phospholipase A2,interleukin-1? and tissue inhibitor of matrix metalloproteinase-1 following traumatic brain injury suggested that they participated in the pathogenesis of the traumatic brain injury,and may played roles in this pathophysiological process.
5.Effect of Tiam 1 on cell proliferation and migration in head and neck squamous cell carcinoma cells.
Ping XIAO ; Quiyang FU ; Zeyong LI ; Xuehui ZHANG ; Xiangjun FU ; Renqiang WAN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(7):633-637
OBJECTIVE:
To investigate the effect of T lymphoma invasion and metastasis 1 (Tiam 1) overexpression in head and neck squamous cell carcinoma (HNSCC) cells.
METHOD:
Endogenous expression of Tiam 1 in 8 head and neck squamous cell carcinoma cell (HNSCC) lines was investigated by real-time RT-PCR. A lentivirus vector containing Tiaml was transfected into UM-SCC-47 cells, a head and neck squamous cell carcinoma cell line with little endogenous Tiaml expression. Stable clone, obtained by G418 screening, were assayed by RT-PCR and Western blot to validate the gene expression efficiency. The biological behaviors of the transduced cells were determined by cell counting, MTT and in-vitro migration assay.
RESULT:
Tiam 1 gene was highly expressed in M2 cell line and it's low level expression was found in UM-SCC-47. Cell counting and MTT assay showed that over-expression of Tiaml significantly promoted cell proliferation (P < 0.05). The cell monolayers overexpressed Tiaml that resulted in a significant increasment of cell migration in infected head and neck squamous cell carcinoma cell lines (P < 0.05).
CONCLUSION
Tiam 1 gene plays an important role in the growth and migration in head and neck squamous cell carcinoma cell lines. It may be a useful marker for metastasis of head and neck squamous cell carcinoma.
Blotting, Western
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Carcinoma, Squamous Cell
;
metabolism
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pathology
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Cell Line, Tumor
;
Cell Movement
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Cell Proliferation
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Guanine Nucleotide Exchange Factors
;
genetics
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metabolism
;
Head and Neck Neoplasms
;
metabolism
;
pathology
;
Humans
;
Squamous Cell Carcinoma of Head and Neck
;
T-Lymphoma Invasion and Metastasis-inducing Protein 1
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Transfection
6.Regulatory Effects of miRNA-31 on LATS2 and Cardiomyocyte Hypertrophy in Rat's Cardiomyocytein vitro
Junyi ZENG ; Wan ZHANG ; Lu DING ; Yunfeng WEI ; Zeqi ZHENG ; Tong WEN ; Yongnan FU
Chinese Circulation Journal 2017;32(2):177-182
Objective: To observe the regulatory effects of miRNA-31 (miR-31) on LATS2 and cardiomyocyte hypertrophy via down-regulating miR-31 expression in rat's cardiomyocytesin vitro. Methods: Rat's cardiomyocytes were isolated and cultured for 10 daysin vitro, according to different intervention methods, the cells were divided into 4 groups:①Blank control group,②AngII intervention group,③Lentivirus with miR-31 inhibitor infection group,④Negative lentivirus infection group. On day-8, gene expressions of MiR-31, LATS2, cardiac hypertrophy ANP and β-MHC were examined by qRT-PCR; on day-10, cell morphology was observed by fluorescence staining. LATS2 protein expression was examined by Western blot analysis. Dual luciferase reporter plasmids were transfected into 293T cells, then luciferase activity was detected to identify the targeting effect of miR-31 on LATS2. Results: Compared with Blank control group, AngII intervention group showed increased gene expressions of miR31, cardiac hypertrophy ANP and β-MHC,P<0.05, enlarged cardiomyocyte surface,P<0.05; while decreased gene and proteinexpressions of LATS2,P<0.05. Compared with AngII intervention group, Lentivirus with miR-31 inhibitor infection group had down-regulated expressions of miR31, cardiac hypertrophy ANP and β-MHC,P<0.05, reduced cardiomyocyte surface, P<0.05; while slightly increased LATS2 gene expression and obviously increased protein expression,P<0.05. Dual luciferase reporter assay presented that relative luciferase activity of TRAF6-3' UTR+miR-146b was significantly decreased than TRAF6-3' UTR+miR-NC,P<0.01 and relative luciferase activity of LATS2-3' UTR+ miR-31 was signiifcantly reduced than LATS2-3' UTR-NC+miR-31,P<0.01. Conclusion: Cardiomyocytes hypertrophy could be reversed at certain degree by down-regulating miR-31; the targeting effect of miR-31 on LATS2 was involved in cardiomyocyte hypertrophyregulation.
7.Contribution of eIF-4E inhibition to heparanase expression at both mRNA and protein levels in human colon adenocarcinoma cell line LS-174T
Yujie YANG ; Shishun ZHONG ; Yanting JIAN ; Tianmo WAN ; Siwu FU ; Xu LI ; Yali ZHANG
Chinese Journal of Pathophysiology 2000;0(10):-
AIM: To determine whether the eukaryotic initiation factor-4E (eIF-4E) inhibition facilitates the degradation of heparanase mRNA and alters heparanase protein expression in human colon adenocarcinoma cell line, LS-174T. METHODS: A 20-mer antisense s-oligodeoxynucleotide (asODN) targeted against the translation start site of eIF-4E mRNA were introduced into LS-174T cells by lipid-mediated DNA-transfection. eIF-4E protein and mRNA levels were detected by Western blot and RT-PCR, respectively. The mRNA levels of heparanase were determined by Northern blot. The alterations of heparanase expression were confirmed by Western blot analysis. RESULTS: The 20-mer asODN against eIF-4E specifically and significantly inhibited eIF-4E protein expression. Following eIF-4E inhibition, a significant reduction of heparanase mRNA was observed on Northern blot, and at the same time, heparanase protein expression significantly decreased as well. CONCLUSIONS: The results indicate that the inhibition of eIF-4E strongly reduces the stability of heparanase mRNA in colon adenocarcinoma cell line, LS-174T and resultes in an apparent reduction in the expression of heparanase protein. [
8.Free superficial iliac circumflex artery skin flap: the clinical application and management of donor site defects.
Jun-Tao HAN ; Song-Tao XIE ; Ke TAO ; Wan-Fu ZHANG ; Peng JI ; Da-Hai HU
Chinese Journal of Plastic Surgery 2013;29(3):175-177
OBJECTIVETo investigate the clinical application of free superficial iliac circumflex artery skin flaps, as well as the management of donor site defects.
METHODS17 free superficial iliac circumflex artery skin flaps were applied for the traumatic defects or deformities on face, neck, foot, hand, ankle and lower leg, respectively. The donor site defects were closed directly or covered by paraumbilical island flaps.
RESULTSThe 17 flap size ranged from 5 cm x 3 cm to 19 cm x 14 cm. 16 flaps survived completely except 1 flap with partial necrosis, which was closed by free skin graft. The donor site defects were closed directly in 10 cases, and covered by paraumbilical island flaps in 7 flaps without no flap necrosis. The abdomen had a good appearance.
CONCLUSIONSGood appearance can be achieved with free superficial iliac circumflex artery skin flaps for the defects on face, neck, foot, hand, ankle and lower leg. Paraumbilical island flap can be used for the donor site defects.
Arteries ; Foot ; Free Tissue Flaps ; blood supply ; transplantation ; Humans ; Reconstructive Surgical Procedures ; Skin ; Skin Transplantation ; Transplant Donor Site ; surgery ; Wounds and Injuries ; surgery
9.Relationship of stress hyperglycemia, cystatin C and glomerular filtration rate with short-term prognosis in elderly patients with acute myocardial infarction
Chongrong QIU ; Jian SUI ; Qian ZHANG ; Peng WEI ; Peng WAN ; Ke ZHU ; Qiang FU
Chinese Journal of Geriatrics 2016;35(2):163-167
Objective To investigate the relationship of stress hyperglycemia,cystatin C and estimated glomerular filtration rate (eGFR) with short-term prognosis in elderly patients with acute myocardial infarction.Methods 242 consecutive patients with acute myocardial infarction were divided into two groups according to age:the elderly group (n=182),and the non elderly group (n=60).The clinical data including cystatin C (Cys C),eGFR and stress hyperglycemia levels were collected.The major adverse cardiovascular events (MACE) were observed during hospitalization and 30 days after discharge.Results The incidences of stress hyperglycemia,the levels of creatinine,Cys C and brain natriuretic peptide (BNP),as well as the total MACE were higher and eGFR was lower in elderly group than in non-elderly group (all P<0.05).Cys C level was positively correlated with age,body mass index and levels of creatinine and BNP (all P<0.05),and negatively correlated with fasting glucose and eGFR in elderly group (both P<0.05).The eGFR was positively correlated with body mass index (P<0.05),and negatively correlated with age,creatinine and BNP levels in elderly group (all P<0.05).Logistic regression analysis indicated that stress hyperglycemia [OR=1.871,95%CI:1.071-3.269,P=0.03],Cys C [OR=7.093,95%CI:2.261-22.249,P=0.00] were the independent risk factors for MACE.Conclusions Cys C level and eGFR can predict the early renal dysfunction and its prognosis in elderly patients with acute myocardial infarction.The incidence of stress hyperglycemia is higher in the elderly,and stress hyperglycemia and Cys C level are the independent risk factors for MACE.
10.Evaluation of right heart function in patients with acute pulmonary embolism by 256-slice spiral pulmo-nary artery angiography
Jinling ZHANG ; Hongwei LIANG ; Fang LIANG ; Bailu LIU ; Deli ZHAO ; Yong WAN ; Xuehui FU
Chinese Journal of cardiovascular Rehabilitation Medicine 2016;25(2):198-202
Objective:To explore value of 256-slice spiral CT pulmonary artery angiography (CTPA)evaluating right heart function in patients with acute pulmonary embolism (APE).Methods:According to death risk assessment of APE patients, a total of 67 APE patients were divided into high risk APE group (n=41)and non-high risk APE group (n=35).CTPA was used to analyze and compare CTPA obstructive index and right heart function indexes between two groups.Correlations among CTPA obstructive index and right heart function indexes were also analyzed.Results:Compared with non-high risk APE group,there were significant rise in CTPA constructive index [8.58% vs.24.69%],percentages of straight or left protruded ventricular septum (31.46% vs.73.17%)and bronchial artery dilation (5.71% vs.24.69%);Except the supe- rior vena cava diameter,the rest right heart function parameters had difference significance in two groups,P<0.05 or<0.01. Spearman correlation analysis indicated that CTPA obstructive index was significant positively correlated with right ventricular maximal short axis (RVMSA),RV:LV,diameters of main pulmonary artery,superior vena cava and azygos (r=0.684~0.954),and significant inversely correlated with LVMSA (r=-0.786),P<0.01 all.ROC curve analysis indicated that AUCs of CTPA RV:LV (0.949)was the biggest diagnosing APE severity.Conclusion:Right heart func- tion indexes measured by multi-detector CT are accurate and practical indexes evaluating APE severity,and it possesses im- portant significance.