Group-specific component(Gc), one of the polymorphic proteins of human plasma,has been isolated and purified from human plasma of Gc 2-2 phenotype by a procedure including DEAE-Sephadex-A50,Sephadex G100 and DEAE-Sephadex-A50 chromatography. The purified Gc identified by PAGE,SDS-PAGE and immunoelectrophoresis was homogeneous and reacted specifically with the commercial anti-Gc serum (DAKO) kit. The molecular weight of the purified Gc was about 58 kd.

Anti-GC serum was successfully prepared in two New Zealand rabbits immunized with GC protein which was isolated and purified from GC2-2 serum previously in our laboratory. The results of identification showed that the specificity of the home made anti- GC serum and the commercial anti- GC serum (DAKOPATTS) were identical. The titer of the home made anti-GC serum was 128. Three common phenotypes, GC1-1, GC2-1 and GC2-2could be identified by immunoelectrophoresis with the home made anti-GC serum. The concentration of GC protein as low as 3.1 ?g/ml could be detected by double immunodiffusion. In addition the anti-GC serum does not cross react with other human serum proteins.

Healthy skin sources from sex hormone, the reproductive health is closely related with skin, they grow and decline together. The methods of regulating menstruation and whites, nourishing kidney for pregnancy clinically can make patients’ skin regain brilliance in the meantime of curing sterility, menstruation disease and pelvic inflammation, from this we can know TCM has broad prospect in beautification.

Objective To investigate the weight loss mechanism of sleeve gastrectomy.Methods SD rats were fed with high-fat diet to induce obesity,and then the obesity rats were randomly divided into experimental group(underwent SG) and control group(underwent sham surgery).Eight weeks after operation,the body weight,food intake and peripheral blood concentration of Ghrelin,GLP-1,PYY3-36 of rats were checked.Results Compared with controll group,in experimental SG model,food intake was decreased(P 0.05).Conclusions The weight loss of sleeve gastrectomy in DIO rats's decline is lasting and obvious.The postoperative effect of decreased Ghrelin and increased GLP-1,PYY3-36 is the main mechanism for weight loss.

Objective To investigate the role of platelet rich plasma (PRP) on free fat graft's survival in a rat model.Methods Twenty-four rats were used in the experiment.For each rat,three groups of free fat grafts were injected into the subcutaneous layer of dorsum.Group A was the control group with 0.5 ml fat and 0.12 ml normal saline.Group B received 0.5 ml fat and 0.1 ml PRP with 0.02 ml normal saline.Group C was given combination of 0.5 ml fat and 0.1 ml PRP which was activated by 0.02 ml calcium chloride.Eight rats were euthanised using pentobarbital sodium each time,1,2 and 3 months after fat transplantation.Specimens were acquired and stained with haematoxylineeosin,and then the histological changes examined using light microscopy.All the data were statistically analysed using paired t test to determine if there were differences between the study groups.Results Compared the volume of the fat grafts at 1 month,there was no deference between the groups(P＞0.05).However,the diameter of group C was significantly higher than group A (P＜0.05).On histological examination,larger vascular density was observed in group C compared with group A at 1 month (P＜0.05).And reduced inflammatory reaction was also observed in group C at 3 months(P＜0.05).While other histological parameters were not statistically significant (P＞0.05).Conclusions PRP does not enhance the survival of fat graft in the Wistar rat model.

Objective To investigate the association between the peroxisome proliferator-activated receptor-or (PPARα) polymorphism rs1800206 (c.484C ＞ G,L162V) and the risk of metabolic syndrome (MES)Methods There were 1184 subjects aged 40-60 years recruited from our hospital between March 2010 and March 2011.The PPARα polymorphism 484C ＞ G was genotyped using TAQMAN assay by real-time PCR. The relationship between PPARα polymorphism and MES risk were investigated.Results The genotype frequencies were 91.4％,8.4％ and 0.2％ for the PPARα CC,CG and GG,respectively.This SNP was in Hardy-Weinberg equilibrium(P =0.845).Compared with the most common CC genotype,the variant genotypes(CG + GG) had higher fasting glucose((5.82 ± 1.59) mmol/L vs (5.49 ± 1.17) mmol/L,t =2.630,P =0.009) and LDL-C levels((3.53 ± 1.03) mmol/L vs (3.36 ± 0.65) mmol/L,t =2.376,P =0.018) and lower HDL-C levels ((1.56 ± 0.35) mmol/L vs (1.65 ± 0.43) mmol/L,t =2.430,P =0.015).Furthermore,the 484G variant genotypes(CG + GG)was associated with the risk of MES after adjusting for age,gender,education,BMI and lifestyle (smoking and alcohol status) (adjusted OR =1.89 ;95％ CI =1.09-3.23,P =0.012).Condusion PPARα polymorphism rs1800206 is a significant independent predictor of the MES in Southern Chinese.

Objective To systematically evaluate the curative effect of sequential treatment and standard triple therapy in eradicating Helicobacter pylori and the risk of adverse events between the two therapies.Methods The related studies were searched with electronic databases to evaluate the efficacy of sequential therapy and standard triple therapy in eradicating H.pylori.Information was retrieved including the clinical features of patients,quality of research,eradication rate of H.pylori and incidence of adverse events and so on.The pooled relative risk(RR)and 95%confidence intervals(95%CI)were calculated,and the publication bias was assessed with funnel plot. Results Twelve randomized trials were performed,and 3183patients met the internalization criterion.Meta analysis demonstrated a clear superiority of the sequential therapy over 7-day triple therapy with an RRof 1.23(95%CI:1.19-1.27),over 10-day triple therapy with aRRof 1.17(95%CI:1.10-1.24),and over 14-day triple therapy with a RRof 1.25(95%CI:1.06-1.48).The incidence of adverse event was similar with sequential therapy versus that of triple therapy(RR=0.96,95% CI:0.75-1.23).Conclusions Sequential therapy is associated with a higher eradication rate of H.pylori compared with 7-day,10-day and 14-day triple therapy.The adverse event doesn't increase significantly.

Objective:To observe the effect of overdose fluoride on the expression of bFGF in rat's dental pulps.Methods:NaF at 20 mg/(kg?d) was given to each of 10 Wister rats by stomach perfusion for 8 weeks, another 10 rats were given the same volume of distilled water as the controls.After treatment,HE staining was used to observe the morphology of the teeth, immunohistochemical staining was adopted for study the expression of bFGF in the dental pulps of the rats.Results:The enamel layer and dentin layer of the incisors in NaF treated rats were thinner than those in the controls,the interglobular dentin was increased and the expression of bFGF in dental pulp and inner dentin was inhibited in the NaF treated rats(P

This paper reports the detection of G2m(n)factors in human sera using theenzyme-linked immunosorbent inhibition test with monoclonal antibodies agai-nst G2m(n)factor(SH-21).The gene freguency of G2m(n)factor among 517unrelated individuals of ban population in Chengeu area was 0.5493 and itsvariance was 0.0004.