Objective To investigate the infection statue of new drug experimenters with hepatitis B,as well as liver fuction in 6 kind of infection modes of serum HBV,and to provide evidence for clinical therapy of hepatitis B.Methods 5 immunological HBV markers were measured with ELISA in serum sample of 550 patients before new drug test. Velocity method was applied to detecting serum TBiL,ALT,AST,γ-GT and GLO.Results New drug experimenters with hepatitis B mainly suffered from "small three positive" [HBsAg (+),anti-HBe (+) and anti-HBc (+)] (52.9%),followed by "big three positive" [HBsAg (+),HBeAg (+) and anti-HBc (+)] (28.2%). The rate of hepatic function abnormality was significantly higher in "small three positive" group than that of the other groups (P<0.01),while the rate of hepatic function abnormality was much higher in "big three positive" group than that of the other groups except "big three positive" group (P<0.05).Conclusion In 550 cases of new drug experimenters,HBV are all positive,mostly in "small three positive" or "big three positive" mode. The rate of hepatic function abnormality is markedly elevated in 6 kind of infected modes,in which "big three positive" mode is the highest,followed by "1,3" mode and"small three positive" mode.

Objective To evaluate early hemopeffusion in the patients of acute organophosphate poisoning from economic.Methods Forty patients with acute organophosphate poisoning were divided into early hemoperfusion group (20 cases) and control group (20 cases) according to the condition of early hemoperfusion in three hours after the diagnosis.The date of basal clinical conditions,treatment,prognosis and cost in the 2 groups were observed.Results There were no statistical differences in sex,age,the level of initial acetylcholine esterase (ChE) and degree of poisoning between the 2 groups (P ＞ 0.05).The patients of the 2 groups underwent at least 1 time hemoperfusion.The time of beginning hemoperfusion in early hemoperfusion group and control group were (1.6 ±0.9) h and (6.4 ±3.6) h,and there was statistical difference (P ＜0.05).The time of ChE recovery and length of stay in early hemoperfusion group were significantly lower than those in control group [(8.5 ± 2.1) d vs.(10.0 ± 1.8) d,(10.5 ± 4.2) d vs.(12.3 ± 5.4) d],and there were statistical differences (P ＜0.05).In early hemoperfusion group 5 cases needed rehemoperfusion,and 4 cases needed mechanical ventilation,in control group were 8 and 5 cases respectively.There was no death in the duration of hospital stay and during 28 d in early hemoperfusion group,but in control group death in the duration of hospital stay was 1 case and death during 28 d was 1 case.The total hospitalization cost,daily treatment cost,health care cost and drug cost in early hemoperfusion group were significantly lower than those in control group [(17 231.75 ± 5 411.45) yuan vs.(21 394.52 ± 6 049.81)yuan,(1 412.25 ±169.33) yuan vs.(1 579.41 ±307.19) yuan,(4 309.22 ±1 490.12)yuan vs.(5 581.71 ± 1 681.00) yuan,(8 223.41 ± 1 426.10) yuan vs.(9 790.02 ± 1 909.24) yuan],and there were statistical differences (P ＜ 0.05).Conclusion The patients of acute organophosphate poisoning could be benefit from early hemoperfusion,because early hemoperfusion can shorten the recovery time of ChE,the length of stay,and reduce the hospitalization cost.

Objective To explore the effect of early airway evaluation in critical patients in emergency room.Methods One hundred and sixty-six critically ill patients with routine intubation,according to the condition of early airway evaluation,were divided into early-estimate group (86 cases) and control group (80 cases).The patients in control group due to illness change needed to build artificial airway,tracheal intubation for the first time was done by the doctor on duty,and before intubation grading evaluation of airway was made.The patients in early-estimate group in emergency room had been early estimated using improved Mallampati classification and Thyromental distance by the doctor on duty.The number of cases with first successful endotracheal intubation,endotracheal intubation time,cases with forecast of difficult airway,the number of cases forecast of difficult airway intubation with first time successful endotracheal intubation,the number of cases failed in intubation and needed emergency tracheotomy,and the time of relatively stable vital signs,emergency department mortality were recorded in two groups.Results The endotracheal intubation time,forecast of difficult airway,rate of failing in intubation and needing emergency tracheostomy tube had no statistical significance (P ＞ 0.05).The first time success rate of tracheal intubation and forecast of difficult airway intubation in early-estimate group were higher than those in control group [69.4％ (59/85)vs.40.0％ (32/80),80.0％ (28/35) vs.32.3％ (10/31)],the time of rehtively stable vital signs was shorter than that in control group[(89.5 ± 23.2) min vs.(110.4 ± 48.2) min],emergency department mortality was lower than that in control group [8.2％(7/85) vs.18.8％(15/80)],and the difference was statistically significant (P ＜ 0.05).Conclusion Early-estimation in the emergency room for the critical patients and preparing for the corresponding work is helpful to improve the success rate of first intubation in the patients with difficult airway,and to restore stability to achieve the safe transfer standard as soon as possible.

Objective To explore the clinical curative effect of Danzhen Headache Capsule on treatment of hypertension with headache.Methods 68 cases of hypertension with headache were divided into treatment group (n =38)and control group(n =30)in accordance with the random number table.The control group received the con-ventional treatment,and the treatment group received addiontional Danzhen Headache Capsule.Clinical curative effects of the two groups were observed.Results After treatment,the treatment group total effective rate was higher than that in the control group(84.21% vs 53.33%).There were statistical differences between the groups(χ2 =7.992,P <0.05),and with non-toxic side effects.Conclusion Proprietary Chinese medicine has curative effect on high blood pressure with headache,Danzhen headache capsule can improve the clinical symptoms as the preferred drug.It is worthy of further clinical research and extension.

OBJECTIVE:To establish the quality standard of Kangshiming mixture.METHODS:The Phellodendron chinense,Citrus anrantium,Paeonia lactiflora,Angelica sinensis,and Pueraria lobata in Kangshiming mixture were identified by HPTLC and the contents of Naringin and Ammonium glycyrrhizinate were determined by UFLC.RESULTS:The spots in the HPTLC were clear and well-separated yet free of interference of negative sample.The linear ranges of Naringin and Ammonium glycyrrhizinate were 0.015～0.135 ?g(r=0.999 9) and 0.1～0.9 ?g(r=0.999 9),respectively,and their recovery rates were 97.78%(RSD=2.19%,n=6) and 101.99%(1.95%,n=6),respectively.CONCLUSION:The established standard is applicable for the quality control of Kangshiming mixture.

Objective To increase the anti-tumor effect of 5-ALA-mediated photodynamic therapy, PDT was performed at 635nm followed by an additional irradiation at 670nm, and the effectiveness was studied. Methods PDT was performed at single irradiation (635nm or 670nm), and symphysial irradiation (635nm+670nm), and its effectiveness was studied using PpIXsolutions, tumor cells (9L and HeLa), andHeLa tumor-bearing mice. Results When a Pp IX solution was irradiated at 635 nm or 670nm, singlet oxygen counts were almost similar. Symphysial irradiation -mediated singlet oxygen generation was higher in 9L cells than HeLa cells and it did not produce an additional PDT effect in HeLa tumors. Conclusion It was suggested as the low uptake of 5-ALA in HeLa cells, and the symphysial irradiation can't increase the anti-tumor effects in 5-ALA-mediated PDT. Further study is necessary to obtain the irradiation method for the efficient application of PDT.

Nosocomial infection is a major challenge in modern hospital management. It affects not only patient safety but also the health of the health care workers. Inappropriate control of nosocomial infections will lead to serious results. China introduced the concept of nosocomial infection control in mid 1980s. Since then, tremendous achievements have been made, while lags still exist when compared with the developed countries. Further efforts should be made to strengthen discipline construction and develop scientific control approaches.
China ; Cross Infection ; prevention & control ; transmission ; Humans ; Infection Control

Objective To investigate the correlation between HPV positive rate and incidence rate of cervical cancer.Methods 438 women,who reveived cervical Pap smears and biopsy,were included in the present study.Human papilloma virus nucleic acid amplification genotyping assay kit was used to detect 21 kinds of HPV subtypes.Results There were 102 patients with cervical cancer (23.29％) and HPV-positive 94 patients (21.46％),which had no significance difference (x2 =0.421,P =0.517).There were 56 patients HPV16 positive (54.90％),19 patients HPV58-positive (18.63％),12 patients HPV18 positive (11.76％) and 8 patients HPV52 positive (7.84％),which were significantly higher than those of other types of HPV-positive rate(all P ＜ 0.05).The proportion of the patients with invasive cervical cancer in HPV i6,HPV58,HPV18,or HPV52-positive was significantly higher than that of intraepithelial lesions and carcinoma in situ (all P ＜ 0.05).Conclusion HPV16,HPV58,HPV18,and HPV52 have high correlation with uterine cancer.

Smoking is one of the major risk factors for stroke. Both active and passive smoking may increase the risks of stroke, and the risks of stroke may decrease after smoking cessation, even no significant difference with non-smokers. Because of the different age, sex, smoking quantity, years of smoking, and regions of the smoking popuation, their strokes and the risks of all stroke subtypes are also different.

Silent brain infarction (SBI) is mainly diagnosed by brain imaging which is more common in the elderly population.It is considered as the early clinical stage of symptomatic cerebral infarction and cognitive impairment.Its risk factors include age,history of smoking,hypertension,diabetes,metabolic syndrome,carotid atherosclerosis,chronic kidney disease,atrial fibrillation,and obstructive sleep apnea.The related serum biological markers include high-sensitivity C reactive protein,homocysteine,cholesterol,uric acid,and soluble intercellular adhesion molecule-1.This article reviews the advances in research on the imaging characteristics,risk factors,serum biological markers and clinical significance of SBI.