Objective To analyze the change in immune function and therapeutic effectiveness of children with im-mune thrombocytopenic purpura and Helicobacter pylori(H. pylori)infection. Methods Eighty hospitalized chil-dren with thrombocytopenic purpura between March 2011 and March 2012 were as observation group,and then sub-divided into infected group and non-infected group according to whether they were infected with H. pylori;80 healthy children were as control group . Lymphocyte subsets ,clinical therapeutic effectiveness and recurrence rate among three groups were compared. Results The positive rate of H. pylori in observation group was significantly higher than control group(55.00% [44/80]vs 2.50% [2/80];χ2= 12.48,P= 0.008). There was significant differences in CD4+,CD4+/CD8+ ,and CD19+T lymphocyte among three groups (all P<0.05);CD4+ and CD4+/CD8+ T lymphocyte in infected group was significantly lower than non-infected group respectively ([13.40±4.65]% )vs [28.56± 3.82]% ;[0.69±0.18]% vs [1.04±0.23]% ),and CD19+T lymphocyte in infected group was significantly higher than non-infected group ([45.21±10.20]% vs [22.05±2.23]% );CD4+ and CD4+/CD8+T lymphocyte in non-in-fected group and infected group was significantly lower than control group ([40.20±3.42]% ,[1.54±0.42]% respec-tively),and CD19+T lymphocyte was significantly higher than control group ([11.02±2.89]% ). The overall therapeu-tic effectiveness in infected group and non-infected group was 90.91% and 91.67% respectively(P>0.05). The recur-rence rate of thrombocytopenic purpura after anti-H.pylori infection therapy in infected group was significantly lower than non-infected group (20.45% vs 30.56% ;χ2= 6.396,P= 0.038). Conclusion Detection of immune function is helpful for clinical diagnosis of H. pylori infection associated with immune thrombocytopenic purpura .

Head and neck surgery is a relatively young discipline. Since 1937,it has made important advances. In the past, the patients had to sacrifice their involved organs in order to survive, but now we have to think not only of the survival rate of the patients,but also should pay more and more attention to improve their quality of life. The plan of treatmant must consider multimodality therapy and function preservation. To attain this goal, we should pay attention to laparoscopic surgery and sentinal node biopsy as well as possibility of gene therapy in head and neck tumors. In addition, we should investigate to application of molecular biology in head and neck surgery.

Objective To establish a HPLC method for the content determination of berberine hydrochloride in Xiaozhi Granule. Methods HPLC was performed on ODS column, the mobile phase consisting of acetonitrile-0.5%acetic acid (20∶80)and detection wavelength at 346 nm. Results The linearity of berberine hydrochloride was good in the range of 3.84～38.4?g/mL (r=0.9998), and the average recovery was 100.52 %, RSD=l.08 %(n=5). Conclusion This method was simple, sensitive and accurate , and can be used for the quality control of Xiaozhi Granule.

Objective To establish a method for the content determination of shikonin in Radix Arnebiae Oil by HPLC.Methods The content of shikonin was determined by HPLC and the chromatographic conditions were: Shim-pack VP-ODS column(4.6 mm?250 mm),the mobile phase consisting of methanol-0.025 mol/L H3PO4(85 ∶15),detection wavelength being 516 nm,tempeature at 25 ℃and the flow rate being 20 ?L.Results A good linearity of shikonin was in the range of 11.2 ?g/mL～33.6 ?g/mL(r=0.9998),and the average recovery rate was 101.03 %,RSD=1.91%.Conclusion This method was simple,accurate and with a good reproducibility and can be used for the quality control of Radix Amebiae Oil.

OBJECTIVE:To establish a HPLC method for content determination of ginsenoside Rg1 in Fufangtianqi dropping pills.METHODS:The separation was performed on a Shim-pack VP-ODS column at the 25℃,the mobile phase was composed of a mixture of acetonitrile-water(25∶75),the flow rate was 1.0 ml/min,the ultraviolet wavelength was 210nm,the samplesize was 20?l.RESULTS:The linear range of gindenoside Rg1 was 37.2～186.0?g/ml(r=0.9 999),and the average recovery was 101.01%(RSD=0.90%,n=5).CONCLUSIONS:This method is simple,sensitive and accurate,and can be available for the quality control of Fufangtianqi dropping pills.

Objective To investigate the expression of ICAM-1 and VCAM-1 in colorectal carcinoma and the rela-tionship between ICAM-1, VCAM-1 and the occurrence, the metastasis of colorectal carcinoma, and to analyze the significance. Methods Immunohistochemistry staining(S-P method) was used to detect the expression of ICAM-1 and VCAM-1 on consecutive section in 78 cases of colorectal carcinoma and normal mucosa and 42 cases of colorec-tal adenomas. Plasma was obtained from 78 patients with colorectal carcinoma and 40 healthy volunteers prior sur-gery, by an enzyme-linked immunosorbent assay (ELISA). Results There was significant difference between the high expression of ICAM-1 and VCAM-1 in colorectal carcinoma and the low expression in normal mucosa and ade-noma(P < 0. 01). Serum ICAM-1 and VCAM-1 were significantly higher in patients with colorectal carcinoma than those in normal control group(P < 0. 01). The ICAM-1 and VCAM-1 levels were not related to sex, age, tumor lo-cation and histological grades(P > 0. 05),but had significantly statistical difference between lymph node metastasis and Dukes stage(P < 0. 01). Conclusion The increase expression of ICAM-1 and VCAM-1 in colorectal cancer suggests that the ICAM-1 and VCAM-1 may play an important role in evaluation of malignant degree and judging the progress as well as the mechanism of colorectal cancer.

Pancreatic neuroendocrine neoplasms (pNENs) can be divided into functional and non-functional.Insulinomas,gastrinomas,glucagonomas and VIPomas are the common types of pNENs.Radical resection is the only way for curing pNENs or for a long-time survival of patients.The basic surgical procedures for pNENs are consist of local resection (tumor enucleation) or anatomical resection [pancreatoduodenectomy (standard or pylorus-preserving pancreatoduodenectomy),distal pancreatectomy (combined with splenectomy or spleen-preserving pancreatectomy) and middle segmental pancreatectomy].Liver is the most common site for metastases.Surgical resection is the method of choice for liver metastases.Radiofrequency ablation,transcatheter arterial chemoembolization and liver transplantation could be the adjunctive therapies.

Both ?-cell dysfunction and insulin resistance contribute to the development of type 2 diabetes, and ?-cell function, especially early insulin secretion, is important in maintaining normal glucose tolerance. This review focuses on the ciritical role of ?-cell dysfunction in the pathogenesis of type 2 diabetes.

Cancer is one of the gene diseases. The genome stability is closely associated with the occurrence of cancers. XRCC1 gene is one of the DNA repair genes. It participates in the repair process when the genome is injured. So it is considered important in maintaining the stability of the genome and in the prevention of tumor occurrence. This article introduced the characteristic of XRCC1 gene, the relationship between single nucleotide polymorphism of the gene and susceptibility to tumor, and the common methods used in the XRCC1 research.

BACKGROUND: Superparamagnetic iron oxide (SPIO) contrast agent has been extensively explore in tracing imaging following stem cell transplantation, arabinogalactan cell membrane receptor targeted imaging, as well as liposome-coated SPIO. OBJECTIVE: To summarize the research state of liposome-coated SPIO. METHODS: A computer-based online search of CNKI and Science Direct (1998-01/2009-09) was performed to search the articles about liposome-coated SPIO with key words "SPIO, superparamagnetic iron oxide, liposome, MR contrast agent" in Chinese and English. Articles related to MR contrast agent, and published in recently or authorial journals of the same field were included. A total of 48 articles were collected, and 24 articles and 1 composition related to liposome-coated SPIO were reviewed. RESULTS AND CONCLUSION: The incessant innovation in MR contrast agents can greatly improve the diagnosis of MRI. Due to the good performances, diagnostic value of SPIO in many diseases has surpassed previous MR contrast agents. Liposome-coated SPIO has the merits of low toxic side effect, and well targeting in specific tissues, thereby extensively being used in experimental studies and clinical application. With the development of functional imaging and molecular imaging, the use of liposome-coated SPIO must be more extensive.