Purpose:Evaluate the prognostic factors in invasive vulvar cancer.Methods:Fifty one cases with invasive vulvar cancer admitted in our hospital from 1980 to 1999 were retrospectively analyzed.Results:Thirty six patients had squamous cell carcinoma and 27 of them were well differentiated and 6 moderately differentiated and 3 poorly differentiated ,three had adenocarcinoma, three had basal cell carcinoma ,three had leimyosarcoma , six had malignant melanoma. Thirteen cases (25.4%) were in FIGO stage Ⅰ;29 (56.7%) in stage Ⅱ;7(13.1%) in stage Ⅲ and 2(3.9%) in stage Ⅳ. The 5-year survival rates of stage Ⅰ,Ⅱ,ⅢandⅣwere 81.5% , 68.4% , 41.2% and 0.0% (P

Objective:To construct a EGFP(Enhanced green fluorescent protein)-labled euk-aryotic expression plasmid of Fcy::Fur suicide gene and to detect its expression in SKOV3 cell line. Methods:With the technology of gene re-arrangement,Fcy::Fur gene in pORF-Fcy:Fur plasmid was subcloned into pEGFP-N1 vector,with its correctness evaluated by the means of r-estriction enzyme analysis and sequencing.It was transfected into SKOV3 cells with lipofectin,the transient expression of GFP was observed under flu- orescence microscope after 24 hours and detected by Western blot. Results:Correct construction of pEGFP-N1-Fcy::Fur was identi- fied by methods of restriction enzyme analysis and nucleotide sequence determination.A total of 60% transfe-cted cells emitted out green fluorescence under fluorescent microscope after 24 h after transfecti-.on. Fcy::Fur gene expressed by the transfected cells were testified by Western blot. Conclusion:The recombinant eukaryotic expression vectors have been constructed successfully and effec- tive-ly expressed in ovarian cancer cells,which may provide an experimental basis for gene therapy of ovarian cancer.

Objective To investigate the correlation between HPV positive rate and incidence rate of cervical cancer.Methods 438 women,who reveived cervical Pap smears and biopsy,were included in the present study.Human papilloma virus nucleic acid amplification genotyping assay kit was used to detect 21 kinds of HPV subtypes.Results There were 102 patients with cervical cancer (23.29％) and HPV-positive 94 patients (21.46％),which had no significance difference (x2 =0.421,P =0.517).There were 56 patients HPV16 positive (54.90％),19 patients HPV58-positive (18.63％),12 patients HPV18 positive (11.76％) and 8 patients HPV52 positive (7.84％),which were significantly higher than those of other types of HPV-positive rate(all P ＜ 0.05).The proportion of the patients with invasive cervical cancer in HPV i6,HPV58,HPV18,or HPV52-positive was significantly higher than that of intraepithelial lesions and carcinoma in situ (all P ＜ 0.05).Conclusion HPV16,HPV58,HPV18,and HPV52 have high correlation with uterine cancer.

Brain tumor stem cells (BTSCs) play a key role in tumorigenesis and tumor development.There are a group of special markers in the surface of brain tumor stem cells,which can sorts stem cells,precursor cells and cancer stem cells.In recent years,the studies of CD133,Nestin,high-mobility group A1,A2B5 have made some breakthroughs.

Objective To observe the clinical effect of controlled ventilation with i-gel laryngeal mask airway in painless bronchoscopy. Methods 80 patients were randomly divided into control group (group A, n =40) and surface anesthesia group (group B, n = 40). Fent-anyl, ruifentanyl, propofol, succinylcholine anesthesia and controlled ventilation was adopted by i-gel laryngeal mask combined with T-seal adapter in control group.Surface of lidocaine anesthesia, spontaneous breathing, nasal cannula oxygen were applied in surface anesthesia group. Patients vital signs change in bronchoscopy , endoscopic related adverse reactions such as body movement, cough, laryngospasm. anesthesia related adverse reactions such as postoperative bucking, choking hoarseness, examination time and patient satisfaction were recorded. Results Controlled ventilation with i- gel laryngeal mask shows airway changes are small (P < 0.05), and less adverse reaction (P < 0.05). Patients showed higher satisfaction (P < 0.05). Conclusion controlled ventilation with i-gel laryngeal mask in painless bronchoscopy is safe , controls the airwayeffectively , and solve the problem of respiratory depression , which proves to be a feasible method.

Objective To explore the relationship between antibiotic application time and delivery outcome after the premature rupture of membranes,and to explore the effect of using antibiotics to delivery outcome.Methods 474 premature rupture of fetal membranes parturient who deliver in our desk work were selected as study objects.According to the time of premature rupture of membranes,the patients were dividwd into groups.The patients that bear children within 12h after the membrane rupture are divided into A group.A group had 284 cases and they were randomly divided into two groups,A1 group and A2 group,both of which had 142 cases.The patients that beared children within 12 ～ 24h after the membrane rupture were divided into B group.B group had 120 cases.The patients that beared children 24h after the membrane rupture were divided into C group.C group had 70 cases.A2 group,B group and C group were all given antibiotic intravenous infusion,A1 group was not given that.The pregnancy outcome was observed.Results B group and A group,C group and A group had significant differences in the mode of delivery,fetal distress,neonatal asphyxia,chorioamnionitis incidence (all P ＜ 0.05).A2 group and A1 group had no significant differences in the mode of delivery,fetal distress,neonatal asphyxia,chorioamnionitis incidence (all P ＞ 0.05).Conclusion If there are clear evidence which can prove maternal have infected within 12h after the membrane rupture,we can choose not to use antibiotics.And whether using antibiotics or not can not affect the delivery outcome.

Objective:To make clear which drugs can cause drug eruption.Methods:Clinical analysis was made based on the patients data regarding the types of the drup eruption and the severity of liver impairment.Results:There are four types of drugs which can cause drug eruption:antibiotics (55.3%),antipyretic analgesic(26.6%)，Chinese medicine (9.6%) and biological preparations (8.5%).The drug eruptions usually manifested as unticaria,measles-like eruption,multiform erythma and stable eruption.Conclusion:The main allergic drugs are antibiotics and antipyretic analgesic.However,attention should also be payed to the Chinese medicine and biological preparations.

Objective To explore the best fat liquefaction prevention measures for gynecological obese patients with abdominal incision.Methods 84 gynecological obese patients with abdominal incision were divided into the observation group(46 cases)and control group(38 cases),according to the negative pressure attract ball placed or not in the subcutaneous fat layer during operation.Patients in both groups were taken abdominal incision physiotherapy.Results The fat liquefaction rate of the observation group was 8.7％,which was lower than that of the control group(26.3％)(x2=4.65,P＜0.05).Conclusion It is very important to keep the incision dry,smooth drainage for prevention and treatment of fat liquefaction in gynecological obese patients after abdominal incision.

ObjectiveTo study the missing items in questionnaire cfidentification for TCM constitution.MethodsThe questionnaires were regarded as study objects,which were independently finished by 1322 participants in Changfeng community who were over 45 years and agreed to participate in.The data'was analyzed by SPSS.ResultsMissing items were found in the questionnaires,with a missing rate of 33.74％ on an overall basis.No statistic difference was noted between male and female (x2=0.67,P＞0.05).The rate of missing items increased as participants' age climbing up to the age of 80,and then decreased.Groups characterized with age and gender showed difference in missing items.89.69％ of the incomplete questionnaires had less than 3 missing items,which concentrated in some specific items.ConclusionAttention should be addressed to the fact that missing items were found in questionnaircs of identification for TCM constitution finished independently by residents.

Many studies indicate that macrophage migration inhibition factor(MIF)is over-expressed in tumor cells,and is involved in the carcinogenesis and tumor development by multiple methods and ways.The complicated molecular mechanisms are not quite clear,and the studies about MIF in digestive tumors,especially in hepatic cell carcinoma become more and more.