Objective:To construct a EGFP(Enhanced green fluorescent protein)-labled euk-aryotic expression plasmid of Fcy::Fur suicide gene and to detect its expression in SKOV3 cell line. Methods:With the technology of gene re-arrangement,Fcy::Fur gene in pORF-Fcy:Fur plasmid was subcloned into pEGFP-N1 vector,with its correctness evaluated by the means of r-estriction enzyme analysis and sequencing.It was transfected into SKOV3 cells with lipofectin,the transient expression of GFP was observed under flu- orescence microscope after 24 hours and detected by Western blot. Results:Correct construction of pEGFP-N1-Fcy::Fur was identi- fied by methods of restriction enzyme analysis and nucleotide sequence determination.A total of 60% transfe-cted cells emitted out green fluorescence under fluorescent microscope after 24 h after transfecti-.on. Fcy::Fur gene expressed by the transfected cells were testified by Western blot. Conclusion:The recombinant eukaryotic expression vectors have been constructed successfully and effec- tive-ly expressed in ovarian cancer cells,which may provide an experimental basis for gene therapy of ovarian cancer.

Purpose:Evaluate the prognostic factors in invasive vulvar cancer.Methods:Fifty one cases with invasive vulvar cancer admitted in our hospital from 1980 to 1999 were retrospectively analyzed.Results:Thirty six patients had squamous cell carcinoma and 27 of them were well differentiated and 6 moderately differentiated and 3 poorly differentiated ,three had adenocarcinoma, three had basal cell carcinoma ,three had leimyosarcoma , six had malignant melanoma. Thirteen cases (25.4%) were in FIGO stage Ⅰ;29 (56.7%) in stage Ⅱ;7(13.1%) in stage Ⅲ and 2(3.9%) in stage Ⅳ. The 5-year survival rates of stage Ⅰ,Ⅱ,ⅢandⅣwere 81.5% , 68.4% , 41.2% and 0.0% (P

Brain tumor stem cells (BTSCs) play a key role in tumorigenesis and tumor development.There are a group of special markers in the surface of brain tumor stem cells,which can sorts stem cells,precursor cells and cancer stem cells.In recent years,the studies of CD133,Nestin,high-mobility group A1,A2B5 have made some breakthroughs.

Objective:To explore the effect of Shenfu Injection (SFI) on expression of Heme oxygenase-1 (HO-1) and iNOS (inducible nitric oxide synthase) in renal failure rats induced by intestinal ischemia-reperfusion (IR) and its possible mechanism. Methods:The model of intestinal ischemia-reperfusion was induced by clamping superior mesenteric artery for one hour and then releasing the arterial clamp for six hours. Wistar rats were randomized into three groups:IR+normal saline group,IR+SFI group and control group (C group). The serum creatinine and blood urea nitrogen were observed respectively. Expression of HO-1 and iNOS in rat kidney tissue was detected by immunohistochemitry and morphometry computer image analysis. The histological change of kidney was observed under light microscope. Results:①Compared with C group,expression of HO-1 and iNOS increased markedly in IR+ normal saline group (P

AIM: To observe the dysfunciton of myocardial nuclear calcium transport in rat myocardial injury and the effects of liqustraxin on it. METHODS: The model of myocardial damage was induced by subcutaneous injection of isoproterenol (ISO,5 mg?kg -1?d -1). Myocardial nuclei were purified with sucrose density centrifugation and identified zymologically. The activity of Ca 2+-ATPase was measured zymologically and calcium uptake was assayed with 45Ca 2+ isotope. RESULTS: Compared with the control,the Ca 2+-ATPase activity of myocardial nuclear membrane in ischemia group(exprimental) was decreased by 18.1% (P

Objective To observe the effect of intellectual activity on the cognitive function of the elderly.Methods The longi-tudinal follow-up study and the cross-sectional study were undertaken among physical examination people.The intellectual activity group (92 cases,more than 60 years old)often to take part in any one or more of the following activities for 5 years and above:pla-ying chess,playing the game of go,playing mahjong,playing cards,playing checkers,calligraphy,painting,reading,etc.The frequen-cy of intellectual activity was more than once per week at least.According to gender,age and educational level,92 cases were screened out as the control group and the control group was not to take part in above-mentioned intellectual activities or occasionally attend.The respondents had no baseline cognitive impairment.MoCA,MMSE,ADL and self-made questionnaire were adopted to conduct the evaluation.One year later when health check-up,the above-mentioned scales were used to evaluate again.The score changes were compared between the control group and experimental group.Results When enrolling,there was no significant differences in the MoCA scores,MMSE scores and ADL scores between the two groups.One year later,the comparison of the MMSE scores and ADL scores between the control group and experimental group had no significant difference (P>0.05).The Mo-CA scores manifested 2 cases (2.2%)of below the critical value of cognitive impairment in the experimental group and 6 cases (6.5%)in the control group,reaching the mild cognitive impairment(MCI)standards,the difference between the two groups showed the statistical significance (P<0.05).Conclusion The intellectual activity may be beneficial for the prevention of cognitive decline of the elderly and the MoCA scale is more suitable for screening MCI.

Objective To explore NF-κB(P65)expression in breast cancer and its clinical significance.Methods Immunohistochemical staining was used to examine the expression of NF-κB(P65)and its relationship with related elinicopathological factors were analyzed in eighty-seven IDC(invasive ductal cancinoma).Results NFκB(P65)expression was significantly correlated with lower tumor grading,maxilary lymph node metastasis,later TNM styng and HER-2 positive(all P＜0.05),but not with tumor size,age of the patient and the state of ER、PR.Conclusion NF-κB(P65)should be risk factors of poor prognosis in IDC.

Objective To study the effect of rosiglitazone (RSG), the agonist of peroxisome proliferator activated receptor γ (PPARγ), on the proliferation and differentiation of rat osteoblasts and the related mechanisms. Methods The identification of rat primary osteoblasts was performed by alkaline phosphatase (ALP) staining and mineralized nodules. The 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay and p-nitrophenyl phosphate (PNPP) assay were used to observe the effects of different concentrations of RSG on proliferation and differentiation of the osteoblasts. The reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of connective tissue growth factor (CTGF) mRNA. The effects of the different concentrations (0,1,2,5,10 and 20 μmol/L) of RSG on TGF-β1-induced CTGF mRNA expression in osteoblasts were detected. Results (1)Different concentrations of RSG could not change the proliferation of osteoblasts (P＞0. 05). (2)Compared with control group, all different concentrations of RSG could suppress ALP activity in osteoblasts (P＜0. 01 ). (3) RSG suppressed the osteoblats CTGF mRNA expression induced by TGF-β1 in a dose-dependent manner (P＜0. 01). Conclusions In vitro, RSG can inhibit the TGF-β1 induced rat osteoblasts CTGF mRNA expression. RSG may play a potential role in preventing the differentiation of the rat osteoblasts.

Objective To investigate the effect of medial collateral ligament reconstruction using semitendinosus autograft.Methods A total of 12 patients with medial collateral ligament injury received knee examinations.Among them,2 had lateral meniscus injury,and received partial resection of the lateral meniscus;2 had cruciate ligament(CL) injury(anterior CL in one and posterior CL in the other),and underwent bone-ligament-bone reconstruction.Medial collateral ligament reconstruction was performed using the semitendinosus tendon autograft ingrowth in the tibial tunnel.Results Primary healing was achieved in all the cases.In 10 of them,the stability of the knee was regained in 6-month follow-up;stress X-ray examination showed that the medial joint space was smaller than the contralateral one by less than 5 mm,and the position of the interference screw was optimal.Of the 12 patients,11 achieved an active knee flexion between 0? and 120?,the other,who had fracture of the lateral condyle of the femur,had a flexion of 90?.Conclusions Semitendinosus autograft provides sufficient tension for medial collateral ligament reconstruction.The fixation of the knee and the closure of the joint capsule can be achieved by using the method.

Objective To evaluate the value of laparoscopy in the treatment of intramural myoma.Methods Totally 120 cases of intramural myoma were randomly divided into laparoscopy(n=52)and open groups(n=68).Clinical data,including operation time,intraoperative blood loss,rates of postoperative pyrexia and postoperative complications,length of hospital stay and follow-up,were compared between the two groups.Results Compared to the open group,the laparoscopy group had significantly longer operation time (106.3?54.9)min vs(66.5?7.3)min,t=5.918,P=0.000],less blood loss 10-300 ml(median,50 ml)vs 20-200 ml(median,50 ml),Z=-1.998,P=0.046],lower rate of postoperative pyrexia 7.7%(4/52)vs 30.9%(21/68),?2=9.608,P=0.002],similar rate of postoperative complications 3.8%(2/52)vs 8.3%(3/36),?2=0.000,P=1.000],shorter hospital stay (7.5?1.4)d vs(10.4?1.2)d,t=-12.201,P=0.000],and similar length of follow-up (9.7?2.3)months vs(9.6?1.8)months,t=0.267,P=0.790] and rate of normal menses 94.4%(17/18)vs 90.5%(19/21),?2=0.000,P=1.000].Conclusions Laparoscopy is as effective as open surgery for intramural myoma.While the laparoscopy leads to better postoperative recovery than the open surgery.