Objective:To define the reference values of several salivary variables for the diagnosis of Sjgren syndrome (SS).Methods:Mixed saliva was collected by standard proceduer.Sodium,potassium concentration,sodium-potassium ratio,total value of sodium and potassium secretion per minute and pH of the saliva samples in 40 patients with SS and 40 healthy controls were measured by automatic biochemical instrument ISE900 and pH meter.The subjects were divided according to the European classification criteria for diagnosis of SS and normal controls.Independent-sample t test were used for statistic analysis.Results:The salivary concentrations of sodium,potassium and sodium-potassium ratio were significantly elevated and the tolal value of potassium per minute and pH were significantly reduced in patients with SS(P

Aloe vera has shown extensive actions, such as anti-tumor, anti-inflammation, anti-bacteria, enhancing immune function, and anti-gastric mucosal lesion. It may be widely developed as medical products.

ObjectiveTo investigate the expression levels of vascular endothelial growth factor (VEGF)in knee synovial membranes and synovial fluids whether it could be a marker for progressive osteoarthritis.MethodsKnee synovial membranes and synovial fluids of patients with osteoarthritis who were underwent total knee arthroplaaty or arthroscopy were collected. They were classified into four groups according to the radiographic grading[Kellegren-Lawrence(K-L) grade]. Appoint K-L-0-grade patients who suffered from menisci injuries only served as controls. The levels of VEGF in the synovial fluid were measured by enzymelinked immunosorbent assay(ELISA) and VEGF-secreting cells were identified by immunohistochemistry.ANOVA was used for statistical analysis. ResultsVarious degrees of inflammation could be found in all the samplesevaluated histologically on HE-stained sections. Synovial tissue inflammation presented as synovial lining thickening, and inflammatory cells infiltration. VEGF expressed in the synovium linings and surrounding blood vessels. The VEGF levels in the synovial fluids were increased accordingly with K-L grades, which reached the peak level in the late stage of osteoarthritis. The levels of VEGF in the synovial fluids were significantly higher in patients with osteoarthritis[from (1181±116), (1632±140) to (2252±216) pg/ml]than in those with menisci injury (P＜0.01); The percent ages positive cell in each groups were (5±4)％ , (9±4)％,(16±6)％ and (21±6)％ respectively, there were significantly differences too (P＜0.01). ConclusionVEGF originated from synovial tissue may play a role in the pathogenesis of osteoarthritis. High levels of VEGF in the synovial fluids can be regarded as the marker of active osteoarthritis.

Objective To prepare in-house calibrator of apolipoprotein A1 and apolipoprotein B with the values are traceable to the WHO-International reference materials SP1-01 and SP3-07.Methods We participated in the Northwest lipid research laboratory (NWLRL) protocol for target value transfer of WHO-IFCC apo A1 and apoB reference materials, and evaluated of comparability of the measurement.The analyses were performed on a Olympus AU 400, apo A1 and apoB were determined by immunoturbidimetric endpoint method.Results Through 3-step study, the precision and accuracy of apo A1 and apoB determination of our company met the NWLRL criteria.Correlation coefficients between obtained and assigned values of NWLRL on 40 individual serum samples were 0.983 for apo A1, 0.987 for apoB, the average absolute bias were 2.7 % for apo A1, 3.0 % for apoB, respectively.Conclusion NWLRL issued the certificate to Zhongsheng Beikong Bio-Technology and Science Inc.for the in-house apo A1 and apoB calibrator values are traceable to the WHO- International reference materials.

AIM: To study the effect of garlicin on protecting nonalcoholic fat liver in rats induced by high fat diet and explore the pathogenesis involved. METHODS: According to the dosage of garlicin and diet, fifty SD rats were randomly divided into five equal groups: normal control group, model control group and garlicin (10, 20, 30 mg/kg) groups. Apart from the rats in normal control group, the rats were all fed with high fat and high cholesterol diet. After 12 weeks, the levels of serum endotoxin (ETX), total cholesterols (TC), triglyceride (TG), superoxide dismutase (SOD), malondialdehyde (MDA), transaminase and free fatty acids (FFA) were detected. The levels of MDA, SOD, glutathione hormone (GSH) in hepatic tissue were also detected. Then the features of live pathology were observed. RESULTS: The levels of ETX, TC and TG in garlicin groups were significantly lower than those in model control group (P

AIM: To investigate the effects of psychological stress on ulcerative colitis rats and to explore the mechanisms of this;meanwhile to investigate the effects of anxiolytic drug on symptoms of ulcerative colitis rats under psychological stress and the mechanisms of it.METHODS: eighty male Wistar rats were randomly divided into four groups(n=20): UC control group;UC stress group;UC+clonazepam group;UC stess+clonazepam group.After UC model was established with immunization,these rats were fightened by cat to set up psychological stress model.During establishing stress model,each groups were respectively received physiologic saline or clonazepam by intragastric administration.After 15d treatment,all rats were sacrified simultaneously.Colon mucosal inflammationand damage were assessed by measuring colon mass,morphologic damage score,colonic MPO and NO activity levels.Morphologic damage score was examined under microscope.Colonic MPO and NO was measured by spectrophotometric method.Serum hydrocortisone was determined by radioimmunoassay(RIA).RESULTS: Morphologic damage score,colonic MPO and NO,serum hydrocortisone of UC stress group were significantly increased compared with those of UC control group;morphologic damage score,colonic MPO and NO,serum hydrocortisone of UC+clonazepam group were decreased markedly vs UC stress group;Little difference was observed between UC+clonazepam group and UC control group.CONCLUSION: Psychological stress can aggravate symptoms of UC.Although benzodiazepine have no anti-inflammatory action,it relieve effects on internal organs of psychological stress and aggravation of symptoms of UC by to effect limbic system and formatio reticularis.

S-Palmutoylatuon un proteun us one of the most umportant kunds of lupud modufucatuon and plays a vutal role un cell sugnal transductuon, metabolusm and other processes, whuch us formed by covalent bundung of palmutuc acud wuth the sulfhydryl group of cysteune resudue un proteun through thuoester bond. In the present study, acyl-buotun exchange reactuon was performed to convert S-palmutuc acud on the hemagglutunun proteun from unfluenza A vurus unto buotun-labeled tag. The buotun-labeled proteun was then enruched by streptavudun beads and further purufued by electrophoresus, followed by un-gel dugestuon. The results showed that the ratuo of buotun concentratuon of the sample wuth hydroxylamune treatment (+HA ) to that of the sample wuthout hydroxylamune treatment (-HA) was larger than 3. Mass spectrometruc analysus of the dugestuon muxture of the enruched hemagglutunun proteun from unfluenza A vurus udentufued two s-palmutoylatuon modufucatuon sutes that were located on carboxyl termunal reguon of hemagglutunun proteun such as Cys562 and Cys565 , respectuvely. Thus research offers a specufuc and effectuve method for large-scale analysus of S-palmutoylated proteuns.

OBJECTIVE: To investigate the effect of C23 down-regulation on proliferation inhibition and apoptosis induced by cisplatin in human osteosarcoma SaOS-2 cells. METHODS: SaOS-2 cells were randomly divided in to 5 groups: a control group, a DDP group, an S+DDP group, an AS+DDP group, and an R+DDP group. Cell growth suppression of each group was analyzed by MTT assay. RT-PCR and Western blot were used to detect the expression of C23, Bcl-2 and Bax mRNA and protein in each group. Morphologic character of apoptosis was evaluated by DAPI staining. The percentage of apoptotic cells was analyzed by flowcytometer assay. RESULTS: C23 antisense oligonucleotides remarkably inhibited the expression of C23 mRNA and protein in SaOS-2 cells (P<0.01). The proliferation of SaOS-2 cells was remarkably inhibited in the AS+DDP group compared with the DDP group (P<0.01). The expression of C23, Bcl-2 mRNA and protein was weaker and that of Bax mRNA and protein was stronger in the AS+DDP group compared with the DDP group (P<0.01). Cisplatin-mediated apoptosis neclei in SaOS-2 cells and the rate of apoptotic cells were higher in the AS+DDP group than those of the DDP group (P<0.01). CONCLUSION Down-regulation of C23 can contribute to cisplatin-mediated proliferation inhibition and apoptosis in SaOS-2 cells.
Antineoplastic Agents ; pharmacology ; Apoptosis ; drug effects ; Bone Neoplasms ; metabolism ; pathology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Cisplatin ; pharmacology ; Down-Regulation ; Humans ; Osteosarcoma ; metabolism ; pathology ; Phosphoproteins ; metabolism ; RNA-Binding Proteins ; metabolism

OBJECTIVE: To investigate the effect and mechanism of herpes simplex virus type 1 (HSV-1) based vector mediated interlukin-1 receptor antagonist (IL-1Rα) gene for knee osteoarthritis in rabbits. METHODS: HSV-1 vectors containing IL-1Rα genes were constructed and injected into the joint space of the osteoarthritis knee in rabbits for 4 weeks. The rabbits were sacrificed, and the knees were lavaged, dissected and the effect of transgene expression was analyzed. Levels of IL-1Rα and IL-1 expression in the recovered lavage fluids were measured with a cytokine ELISA kit. Cartilage from the lesion areas of medial femoral condyle and synovium were observed with hematoxylin and eosin (cartilage and synovium) and toluidine blue (cartilage). The blank control group was injected pHSV-LacZ vector into rabbit knees. RESULTS: Intra-articular delivery of pHSV-IL-1Rα-LacZ resulted in a significant inhibition of IL-1 level and cartilage degradation compared with those in the blank control group (P<0.05). CONCLUSION pHSV-LacZ is an ideal vector to mediate intra-articular gene delivery in the rabbit model of osteoarthritis. Continuous intra-articular expression of IL-1Rα can treat knee osteoarthritis by inhibiting IL-1.
Animals ; Arthritis, Experimental ; therapy ; Female ; Gene Transfer Techniques ; Genetic Therapy ; methods ; Genetic Vectors ; Herpesvirus 1, Human ; genetics ; Injections, Intra-Articular ; Interleukin 1 Receptor Antagonist Protein ; genetics ; Male ; Osteoarthritis, Knee ; therapy ; Rabbits

Background and objective The therapeutic effect and side effect of neoadjuvant chemotherapy were still disputing issues when applied to resectable Ⅲa stage non-small cell lung cancer (NSCLC) patients. The retrospective analysis was aimed to investigate the short-term efficacy and postoperative complications in resectable Ⅲa NSCLC patients treated with neoadjuvant chemotherapy.Methods According to inclusion criteria and exclusion criteria, 370 patients with clinical diagnosis of ⅢaNSCLC were selected from our hospital between January 2011 and October 2013 were retrospectively analyzed. According to treatment method, group A (preoperative neoadjuvant chemotherapy+surgery group) contained 97 cases, and 273 patients were included in group B (direct surgery without neoadjuvant treatment group). The clinical data, surgi-cal approach, main postoperative complications and disease-free survival (DFS) among patients in two groups were recorded. Results The total down-staging in group A was 65.98% (64/97), the R0 resection in group A and group B were 96.91% (94/97) and 90.48% (247/273), respectively. The operation time, bleeding, postoperative hospitalization were no statistical difference (P>0.05), and the main postoperative complications of the patients in two groups were 76.29% (74/97) and 72.52% (198/273) (P>0.05). All patients followed-up for 2 months-36 months, the median follow-up time was 12.7 months, the total recurrence and metastasis rates were 63.92% (62/97) and 94.87% (259/273) (P<0.05) and the median DFS were 19.46 months and 11.34 months (P<0.001).Conclusion Neoadjuvant chemotherapy can benefit patients of Ⅲa stage NSCLC partly in down-staging T and N stage in tumor, enhance the R0 resection, but not significantly increased the postoperative complications of the patients, which can reduce the local recurrence and metastasis, enhance the DFS effectively.