Objective To compare the advantages and disadvantages of the amplification refractory mutation system -polymerase chain reaction (ARMS-PCR) with restriction fragment length polymorphism polymerase chain reaction(RFLP-PCR) technology, then explore its application in detection and analysis of single nucleotide polymorphism (SNP). Methods By using ARMS-PCR and RFLP-PCR the polymorphisms of the MMP-2 in cervical cancer and the normal-control group were analyzed. The research sites were-1306 C/T.The final result was verified by DNA sequencing. Results The results of amplified used ARMS-PCR showed that the genotypes of the SNP site in MMP-2 gene promoter were CC, CT, TT, and the DNA fragments were corresponding with the results of DNA sequencing. Conclusion ARMS-PCR technology has the advantages of lower cost time-saving simple operation and reliable results, while has a high value for research of SNP.

Objective To investigate the effects of the memory intervention on dysmnesia of elder people in 6 months as well as the intervention method and intervention duration. Methods 100 elderly people in Tangshan communities with memory impairment, with score less than 22 by Rivermead Behavioural Memory Test Second Edition( RBMT Ⅱ) ,were randomly selected and divided into the intervention group(n=50) and control group( n =50). The intervention group were given personalized memory training while the control group just went its normal course. Each group was evaluated with score by RBMT Ⅱ after 3 and 6 months respectively. All the data were made analysis of variance and t test using SPSS 13.0 software. Results After 6 months of intervention, the evaluation scores in the following aspects of the standard score, immediate story recalling, immediate route recalling, letter recalling,orientation, date and appointments recalling, delayed story recalling, name recalling and the hidden recalling were significantly higher than those of control group(P＜ 0. 01 or P＜ 0.05 ). The scores in the standard score,immediate story recalling, imformation recalling, appointments recalling, delayed story recalling, name recalling after 6 months of intervention were significantly higher than those before intervention(P＜ 0.01 or P＜ 0.05). The RBMT Ⅱ score increased significantly from (17.10±2.38 ) before the intervention to ( 19.90 ±2.17) after 3 months of intervention and to( 21.63 ± 1.45 ) after 6 months (P＜0.01). Conclusion Memory intervention is an effective method worthy to be widely spread in the communities in improving the memory of elderly people with dysmnesia.