Animals ; Cerebral Cortex ; cytology ; physiology ; Delayed Rectifier Potassium Channels ; physiology ; Neurons ; physiology ; Patch-Clamp Techniques ; Rats ; Rats, Sprague-Dawley

Medical ethics has its own characteristics on curriculum setup,teaching goals,the structure of teaching staff in America,France and China,It is beneficial for our feform in education in medical ethics to learn and refer to the experiencs of the success abroad.

The rational design of teaching contents of physical pharmacy plays an important role in the class teaching.Some practices of designing teaching contents are expounded in detail,including the combination with teaching cases,the integration of the teaching contents,the combination with the research results of the physical pharmacy,and the combination with the pharmaceutical experiments of traditional Chinese medicine.The innovation of this research is the combination of theory and practice and the infiltration between teaching and scientific research.It also puts forward some measures such as continually accumulating and improving the related material,and constructing a rich,shared source material storehouse,etc..

Angioplasty, Balloon, Coronary ; methods ; Coronary Artery Bypass ; methods ; Coronary Vessels ; surgery ; Humans

Objective To investigate the status of urogenital Ureaplasma urealyticum (Uu) infection and their drug tolerance in our area,so as to guide rational administration for the clinic.Methods Mycoplasma culture and drug susceptibility test were performed for 771 patients with urogenital infaction. The culture,identification and drug resistance of mycoplasma were carried out with one complex mycoplasma kit.Results The positive rate of Uu was 49.9%. The proportional percentage of males was 12.5% and the Uu positive rate was 22.3%,for females,the proportional percentage and Uu positive rate was 87.5% and 60.6% respectively. The population aged 21-40 was susceptible to Uu infection. The most sensitive antibioties to Uu was clindamycin (95.12%),followed by doxycycline (87.57%) and roxithromycin (86.24%).Conclusion The difference of Uu drug sensitivty was significant in different areas. So it is necessary to select antibioties by drug sensitivity. Clindamycin,doxycycline and roxithromycin may are used as preferred antibioties in treating Uu infection in our area. The drug-resistance status of spectinomycin and lincomycin was serious.

Objective:To make clear which drugs can cause drug eruption.Methods:Clinical analysis was made based on the patients data regarding the types of the drup eruption and the severity of liver impairment.Results:There are four types of drugs which can cause drug eruption:antibiotics (55.3%),antipyretic analgesic(26.6%)，Chinese medicine (9.6%) and biological preparations (8.5%).The drug eruptions usually manifested as unticaria,measles-like eruption,multiform erythma and stable eruption.Conclusion:The main allergic drugs are antibiotics and antipyretic analgesic.However,attention should also be payed to the Chinese medicine and biological preparations.

Objective To investigate the efficacy of pulse index continuous cardiac output (PICCO) monitoring in maintaining adequate blood volume in patients undergoing off-pump coronary artery bypass grafting (OP-CABG).Methods Forty-seven ASA Ⅰ-Ⅲ patients aged 43-64 yr weighing 50-85 kg undergoing OP-CABG were included in this study. Global end-diastolic volume (GEDV), extravascular lung water (EVLW), global end-diastolic volume index (GEDVI), MAP, cardiac output (CO) and cardiac index (CI) were monitored by using PICCO monitor before induction of anesthesia (baseline), at the end of operation and at 4 and 8 h after operation. Blood volume was maintained during operation by adjusting the volume and infusion rate of succinyl gelatin and lactated Ringer's solution according to volume indexes and hemodynamic indexes. GEDVI was maintained at 600-800 ml/m2 and Hct≥30% by transfusion of whole blood or erythrocyte suspension;MAP was maintained at 70-90 mm Hg by intravenous infusion of nitroglycerine (0.5-1.0 μg·kg-1·min-1) or diltiazem (2-6 μg·kg-1·min-1); HR was maintained at≤75 bpm by intravenous dopamine infusion (3-5 μg·kg-1·min-1). The oxygen delivery index(DO2 I) and oxygen consumption index (VO2 I) were calculated. Arterial blood samples were taken for blood gas analysis. Results The CI, DO2I and VO2I were increased at the end of operation, and at 4 h and 8 h after operation as compared with the baseline values before induction of anesthesia (P<0.05 or 0.01). Conclusion Perioperative blood volume can be well maintained with PICCO monitoring in patients undergoing off-pump coronary artery bypass grafting.

Objective To detect DNA lesions induced by complex pollutants existing in environment using the technique of randomized terminal linker,dependent PCR (RDPCR). Methods SD rats were treated with organic concentrate by injection i.p., DNA were extracted from rat's liver, kidney, lung and leucocytes, then RDPCR was used to detect DNA damage of exon 7 of p53 gene. Results Organic concentrate of drinking water could make rat's genomic DNA broken. Hybridization bands were found in liver and kidney tissues after RDPCR, none in blood and lung tissues. Conclusion Organic concentrate of drinking water can cause the DNA lesions of exon 7 of p53 gene and the major target organs are liver and kidney. The technique of RDPCR can be used to detect DNA damage induced by complex environmental pollutants.

Objective To predict the HLA A *0201 restricted CTL epitopes (nonamers) in SARS coronavirus (SARS CoV) E protein. Methods The CTL epitopes of E protein were predicted by using supermotif method combined with quantitative matrix method. Results Thirteen HLA A *0201 restricted CTL epitope candidates were predicted in SARS CoV E protein. Conclusion The prediction of the CTL epitopes in SARS CoV E protein will benefit the identification of CTL epitopes by experiment. Those results are of importance for immune recognition and vaccine design for SARS CoV.

Heart Defects, Congenital ; complications ; surgery ; Humans ; Hypertension, Pulmonary ; etiology ; surgery