OBJECTIVE: To validate the method for microbial limit test of Niuhuang jiedu tablet.METHODS: The recovery rates of 5 kinds of test organisms including E.coli treated by Niuhuang jiedu tablets were determined by routine method,culture medium dilution method,centrifugation and membrane-filter method,respectively.RESULTS: The centrifugation and membrane-filter method was suitable for determination of the bacterial number of Niuhuang jiedu tablets,while routine method was applicable for determination of bacterial numbers of mold and yeast and test of control bacteria in the microbial limit test.CONCLUSION: The contaminated bacteria in Niuhuang jiedu tablet can be detected accurately by the method established in this study.

Objective In order to provide a large quantity of retinal pigment epithelial cells (RPE cells) in vitro, we want to estab-lish method for culturing pig RPE cells. Methods RPE cells were separated with trypsin and cultured in vitro. The cells were identified by immunohistochemical staining with anti-human keratin and transmission electron microscope (TEM). Results Cultured RPE cells gradually presented transparent and fusiform shape. Immunohistochemical staining demonstrated that the cells were stained by anti-human keratin, and the cultured cells showed typical ultrastructure of RPE cells. Conclusion The cultured cells may be the foundation of pig RPE cells.

Objective To study the curative effect of traditional chinese medicine combined with noninvasive ventilation on respiratory failure in patients with acute exacerbation of chronic obstructive pulmonary disease.MethodsThe patients were divided into experimental group (n=37) and control group (n=37).The experimental group was treated with traditional chinese medicine combined with noninvasive ventilation.The control group was treated with noninvasive ventilation method.The heart rate, blood gas analysis index, ventilation index and complication were compared between the two groups before and after treatment.ResultsAfter treatment, the levels of PaO2 in the two groups were not significantly different.The heart rate and PaCO2 of the experimental group were significantly lower than those of the control group, while the pH value and SaO2 were significantly higher than those in the control group (P< 0.05), and the total incidence of complications was 21.62%, which was significantly lower than that of the control group (64.86%, P<0.05).The time of infection control window, the highest PEEP and the time of mechanical ventilation were significantly shorter than those of the control group (P< 0.05).ConclusionThe treatment of patients with acute exacerbation of chronic obstructive pulmonary disease(COPD) combined with noninvasive ventilation therapy can significantly improve the heart rate and blood gas analysis indexes, and optimize the noninvasive ventilation efficiency, control lung infection as soon as possible, shorten the time of noninvasive ventilation and reduce the side effects that noninvasive ventilation brought about.

The distribution of ABH substances in human tissue cells was studied using the specific red cell adherence test(SRCA test).Tissues were taken from 11 cadavers of known ABO type and secrete status,fixed with 10% neutral formalin,Isolated abdominal skins kept in room temperature for 1～13 days were also observed for the purpose of studing the influence of the time elapsed after death on SRCA test results ABH substances were found in mucous membranes,mucous glands and prostate glands.ABH substances in those tissues were controlled by secrete status.ABH substances were also found in endothelia of blood vessels,stra- tified epithelia,acinar cells of pancreas and sweat glands.We firstly found that ABH substances were present in epithelia of pulmonary alveoli and epi- thelia of small bile duct in liver. Using SRCA test,the ABO blood type were correctly demonstrated in 11 isolated abdominal skins kept in room temperature for 1～13 days.

AIM To explore the effect of ACE inhibitor on early kidney hypertrophy and its mechanism in diabetic rats. METHODS Rats were randomly divided into three groups: uninephrectomized rats, streptozotocin induced diabetic rats and diabetic rats treated with benazepril (an ACE inhibitor, 10 mg?kg -1 ?d -1 , ig). Activity of ACE was determined by the fluorimetric assay. Expression of TGF? 1 mRNA and TGF? 1 and p21 CIP1 protein was measured by Northern blot analysis and Western blot analysis, respectively. RESULTS After 1 week, the diabetic rats developed a body weight loss, kidney weight/body weight increased and renal cortex ACE activity elevated despite a decrease in plasma ACE activity. Northern blot analysis showed that renal cortex TGF? 1 mRNA expression in the diabetic rats was enhanced by 1.3 times, compared with uninephrectomized rats. Western blot analysis showed that TGF? 1 and p21 CIP1 protein expression were also increased. Administration of benazepril for one week significantly suppressed kidney hypertrophy. ACE activity in the plasma, renal cortex and medulla was reduced by 89%,70% and 70 5%, respectively. Expression of TGF? 1 mRNA as well as expression of TGF? 1 and p21 CIP1 protein was reduced by 47 7%, 49 5% and 60 0%, respectively. CONCLUSION Our results suggest that the suppression of ACE inhibitor on diabetic kidney hypertrophy might partially be associated with a decrease in the expression of TGF? 1 and p21 CIP1 in diabetic rats renal cortex. However, its exact mechanism remains to be further explored.

To prepare simvastatin nanostructured lipid carriers ( simvastatin-NLCs) . Methods:The simvastatin-NLCs were prepared by melt-emulsion ultrasonication and low temperature-solidification methods. Using the particle size, polydispersion in-dex, encapsulation efficiency and drug loading as the idices, the ratio of solid to liquid, lipid concentration, ratio of surfactant to cosur-factant, emulsifier concentration and drug concentration were optimized. The optimized simvastatin-NLCs were characterized for the en-capsulation efficiency, particle size, zeta potential and morphology. In vitro drug release behavior and stability of NLCs were also stud-ied. Results:The optimized simvastatin-NLCs formula was as follows:the concentration of simvastatin, cetyl palmitate, Miglyol? 812, soy lecithin and solutol HS15? was 0. 5%, 1. 5%, 4. 5%, 2. 5% and 1. 5%, respectively. The particle size and zeta potential of NLCs was (102. 2 ± 42. 1) nm and ( -33. 1 ± 4. 1) mV, respectively. The simvastatin-NLCs were found to be small and spherical with smooth surface under a transmission electron microscope. The in vitro release profile indicated that the accumulated release of sim-vastatin reached up to (59. 1 ± 4. 8) % in 24 h. The stability studies showed that simvastatin-NLCs were stable in 3 months after stored at 5℃. Conclusion:The formula of simvastatin-NLCs prepared by melt-emulsion ultrasonication and low temperature-solidifica-tion method is feasible.

Objective To explore the relation between single umbilical artery with other malformations and pregnancy outcome,and to provide evidence for pregnancy counseling and diagnosis.Methods The outcomes of 92 fetuses with single umbilical artery diagnosed and delivered in Beijing Obstetrics and Gynecology Hospital Affiliated to Capital Medical University from September 2007 to July 2009 were followed up and retrospectively analyzed with Chi-square test.Results Among 25 292 pregnant women,92 cases were finally diagnosed as single umbilical artery giving the incidence of 0.36％,among which 57.6％ (53/92) of newborns survived without malformations,15.2％(14/92) survived with malformations and 27.2％ (25/92) died during perinatal period.There were 36 fetuses (39.1％,36/92) with single umbilical artery complicated with other malformations,among which 29 had single malformation (80.6 ％ ),and seven ( 19.4 ％ ) had multiple malformations.The incidence of heart defects was the highest among the complicated malformations,and then followed by central nervous system,digestive system,motor system and urinary system.Thirty-three fetuses with single umbilical artery accepted karyotype analysis.Chromosome abnormality was diagnosed in three subjects (9.1％,3/33).Twenty-three fetus (25.0％,23/92) associated with small for gestational age,and among which 18 fetuses (78.3％,18/23) died with a higher mortality than that (4.0％,24/597) of small for gestational age patients without single umbilical artery (x2=181.71,P＜0.01).Conclusions Single umbilical artery is likely to be complicated with congenital anomalies.After single umbilical artery was diagnosed by routine ultrasound,fetal ultrasonography,echocardiography and amniocentesis for karyotype analysis is suggested.Fetal growth restriction is an important indicator of fetal adverse outcomes.

This review snmmarized and estimated the management of cataract blindness in China recently,and introduced the purport of clinic medical economics.Based on it,it was proposed that our service system of cataract blindness could use the experiences of other countries as reference and engaged in more investigations in search of Chinese way for cataract blindness.

Objective To investigate the effect of Mongolian medicine naru-3 on the apoptosis of mouse hepatic cells induced by radiation. Methods Kunming mice were radiated with 60Coγ-irradiation, the 30-day survival rate of mice and the average life span of dead mice post-irradiation were observed. The apoptosis of hepatic cells from irradiatied mice was detected by FACS and TUNEA expression level of p53 was examined. Results Administration of the medicine resulted in increase of 30-day survival rate and prolongation of average life span of the dead mice. The apoptosis rate of spleen cells from mice treated with Mongolian medicine naru-3 decreased significantly and the ex pression level of p53 was also inhibited significantly. Conclusion The results indicated that Mongolian medicine naru-3 had radio protective function and its mechanism might be related to the suppression of apoptosis of radiationsensitive cells.

Objective To study the superiority and safety of cup-shaped womb manipulator auxiliary peritoneoscope in entire womb excision. Methods 180 cases with hysterectomy were randomly divided into cup-shaped womb manipulator auxiliary peritoneoscope group(treatment group) and tradition into the abdominal group( control group) ,and the amount of bleeding and postoperative complications,and patient recovery were observed in the two groups. Results Compared with the control group, the quality of life of patients with anal exhaust time, average length of staying, postoperative recovery daily time were significantly improved, and there were statistically significant differences between two groups ( P ＜ 0. 05 ). There was no serious complication in the two groups. Conclusion Cup-shaped womb manipulator auxiliary peritoneoscope hysterectomy was a safe and feasible operation,and could relieve harm and improve patients quality of life.