OBJECTIVE: To validate the method for microbial limit test of Niuhuang jiedu tablet.METHODS: The recovery rates of 5 kinds of test organisms including E.coli treated by Niuhuang jiedu tablets were determined by routine method,culture medium dilution method,centrifugation and membrane-filter method,respectively.RESULTS: The centrifugation and membrane-filter method was suitable for determination of the bacterial number of Niuhuang jiedu tablets,while routine method was applicable for determination of bacterial numbers of mold and yeast and test of control bacteria in the microbial limit test.CONCLUSION: The contaminated bacteria in Niuhuang jiedu tablet can be detected accurately by the method established in this study.

Objective In order to provide a large quantity of retinal pigment epithelial cells (RPE cells) in vitro, we want to estab-lish method for culturing pig RPE cells. Methods RPE cells were separated with trypsin and cultured in vitro. The cells were identified by immunohistochemical staining with anti-human keratin and transmission electron microscope (TEM). Results Cultured RPE cells gradually presented transparent and fusiform shape. Immunohistochemical staining demonstrated that the cells were stained by anti-human keratin, and the cultured cells showed typical ultrastructure of RPE cells. Conclusion The cultured cells may be the foundation of pig RPE cells.

Transjugular intrahepatic portosystemic shunt (TIPS) has been developed for two decades in order to treat portal hypertension accompanying with upper gastrointestinal hemorrhage and refractory ascites.TIPS may cause the development of hepatic encephalopathy (HE).TIPS induces portalsystemic shunt and leads to hemodynamics chaos. Hepatic encephalopathy belongs to the complications of the portal hypertension. There are many possible predictors of the post-TIPS HE. The relationship between TIPS and HE was reviewed.

Anti-Arrhythmia Agents ; therapeutic use ; Arrhythmias, Cardiac ; drug therapy ; Child ; Humans ; Sotalol ; therapeutic use

Objective To investigate the correlation between hyperuricemia and obesity in children.Methods 316 children who went to the hospital for treatment of obesity were enrolled in the study as obesity group.Age and sex-matched children with normal body mass index(BMI)were randomly recruited as control group.Fasting venous blood samples were collected for the detection of serum uric acid(UA),glucose(GLU),total cholesterol(CHOL),triacylglycerol (TG),high-density lipoprotein cholesterol(HDL-C) and low-density lipoprotein cholesterol(LDL-C).Results Compared with the control group,serum concentrations of UA,CHOL, TG and LDL-C were higher while HDL-C was lower in obese children,the difference was statistically significant(P <0.05 ).The detection rate of hyperuricemia in obese children was 24.68%,and in control group was 9.49%,the difference was statistically sig-nificant(P <0.05 ).Serum UA concentration was positively correlated with TG,CHOL and LDL-C,negatively correlated with HDL-C.Conclusion Obesity can lead to higher serum UA concentration and abnormal lipid metabolism.The level of serum UA was correlated with abnormal lipid metabolism.

OBJECTIVE:To evaluate the application effects of PDCA cycle management in the outpatient prescription intervention.METHODS:A total of outpatient prescriptions (pre-intervention group) during Jan.-Dec.2014 and 918 659 (intervention group) were selected during Jan.-Dec.2015 were selected from a hospital.Irrational 9utpatient prescriptions were analyzed before and after the application of PDCA cycle management,and the improvement of main indexes were compared such as unsuitable usage and dosage,incomplete clinical diagnosis writing,no physicians' signature on the prescription or inconsistent with sample.RE SULTS:Before intervention,there were 2 347 irrational prescriptions,including 1 401 nonstandard prescriptions,849 unsuitable prescriptions and 97 abnormal prescriptions.After intervention,there were 1 161 irrational prescriptions,including 695 nonstandard prescriptions,425 unsuitable prescription and 41 abnormal prescriptions,decreasing 50.53％,50.39％ 49.94％ and 57.73％,respectively.The proportion of unsuitable usage and dosage,incomplete clinical diagnosis writing,no physicians' signature on the prescription or inconsistent with sample,nonstandard prescription paper,unsuitable indications,no signature and date for prescription revision or no reason and new signature for overdose use,outpatient prescriptions more than 7 d common dose or emergency prescriptions more than 3 d common dose without special situation in total amount of outpatient prescriptions decreased from 0.72‰,0.57‰,0.45‰,0.27‰,0.20‰,0.19‰ and 0.15‰ before intervention to 0.32‰,0.25‰,0.19‰,0.11‰,0.09‰,0.08‰ and 0.07‰ after intervention.CONCLUSIONS:PDCA cycle management significantly improves the quality of outpatient prescriptions.There still are irrational outpatient prescriptions in this hospital,and it is to be intervened continuously.

15℃ . Conclusion The changes of density of different strains of Escherichia coli in the undernourished water samples closely associated with the strain ' characteristics, environment temperatures and preserving time, showed significantly differences.

OBJECTIVE:To establish an LC-MS method for the determination of the ritodrine content in human plasma.METHODS:The determination was performed on Agilent Eclipse Plus C18 column with the column temperature kept at 40 ℃.The mobile phase consisted of methanol-water(95∶5,containing 0.02 mol?L-1 ammonium formate)with a flow rate of 0.5 mL?min-1.The extraction agent was acetic ether-dichloromethane(4∶1).A triple quad mass spectrometer system equipped with an electro spray ionization ion-trap source was used as the detector and operated in the positive ion model.Selected reaction monitoring(SRM)was employed to detect ritodrine(m/z 288.2→121.1)and the internal standard dobutamine(m/z 302.1→107.0).RESULTS:The linear range of ritodrine was 0.25～100 ng?mL-1(r=0.999 1),with the lower limit of quantification at 0.25 ng?mL-1.Both the intra-day RSD and inter-day RSD were less than 15%.The methodological recovery was 98.00%～103.44%.CONCLUSION:The method is sensitive,accurate,reproducible,specific and suitable for the pharmaceutical study of ritodrine.

OBJECTIVE:To provide references for the improvement of civil relief system for adverse drug reactions.METHODS:The approaches for Japan in handling the "quinoform" drug injury event were compared with the way of China in dealing with drug injury events.RESULTS & CONCLUSION:The approaches for Japan in handling the"quinoform" drug injury event give some hints for China,i.e.the defective drugs should be comprehensively judged by judges,the causal relations should be determined by using the probability theory and the time point of producers' exemption should be revised.

OBJECTIVE:To establish a HPLC method for the simultaneous determination of quercitrin and isoquercetin in hypericum japonicum thunb.injection.METHODS:Merck Lichrospher RP 18 was used as chromatographic column and the mo-bile phase consisted of acetonitrile-(pH=3.0)buffer phosphate(18∶82)with flow rate at1ml/min,column temperature at25℃and detection wavelength at350nm.RESULTS:The linear range for quercitrin and isoquercetin were0.278?g～4.440?g(r=0.9991)and0.315?g～2.520?g(r=0.9991),respectively.The average recovery were98.60%(RSD=2.23%)and97.79%(RSD=1.74%),respectively.CONCLUSION:This method is accurate,reliable and applicable,and suitable for the quality control of hypericum japonicum thunb.injection.