The purpose of this work was to reestablish the respiratory abduction of the paralyzed vocal cord through reinnervation of the posterior cricoarytenoid (PCA) muscle by partial phrenic fibres.In fifteen adult cats the adductor branch of the recurrent laryngeal nerve (RLN) of the right side was cut and its distal end ligated, while its proximal end was implanted into the PCA muscle belly.The whole RLN was then transected in the tracheo-esophageal groove and its distal stump anastomosed to the upper branch of the phrenic nerve.Direct laryngoscopy showed that the inspiratory abduction of the paralyzed vocal cord recovered within 40 d in all cats.80 d later, a larger abducent motion of the glottis was observed on the reinnervated side.Abduction was caused by reinnervation of the PCA muscle from phrenic motoneurons, as demonstrated by laryngeal electromyography and histological testings.The function of the diaphragm was preserved as revealed by monitoring of the intrathoracic pressure.

Recently imageless navigation systems have been developed rapidly, and have become major navigation system in clinic. It comprises computer workstation, infrared optical localizing system, foot control pedal and navigation software. Currently, imageless navigation systems have been used successfully in total knee arthroplasty, uni-compartment arthroplasty, total hip arthroplasty, hip surfacing arthroplasty, total shoulder arthroplasty, anterior cruciate ligament reconstruction and peri-articular osteotomy of knee, etc. The first OrthoPilot imageless navigation system was used by Saragaglia et al in total knee arthroplasty in January 1997, and many clinical studies have been conducted. The clinical results show that this kind of systems greatly improves operative accuracy, and reduce cases beyond ideal range. It shows accuracy as CT-based navigation systems, and they can be commonly used as standard navigation systems in clinic.

Objective To observe the clinical effect of interferon -1 b (Yundesu)in the treatment of verru-ca planae.Methods 1 1 0 patients with verruca pianae were randomly divided into observation group and control group,55 cases in each group.The observation group was given Yundesu injection 30 g once every day in first week, and once every other day in another three weeks.The control group was given orally mannatide 1 0ml two times every day.The leision changes of the two groups were observed after 4 weeks therapy.Results The total effective rate of the observation group was 92.7%(51 /55),which of the control group was 72.78%(40 /55),the difference between the two groups was statistically significant (χ2 =7.70,P <0.01 ).No adverse reactions in the two groups were observed.Conclusion The clinical effect of Yundesu is more prominently beneficial to patients with verruca planae.

Humans ; Skull Base ; anatomy & histology ; surgery

Hearing ; Humans ; Neuroma, Acoustic ; surgery ; Neurosurgical Procedures ; methods

China ; Hearing Tests ; Humans ; Infant, Newborn ; Neonatal Screening

Molecular targeted therapy (MTT) refers to a new therapy designed to interfere with a specific molecular target or signaling pathway that may have a significant influence in tumor growth or progression. MTT can inhibit cell proliferation and induce apoptosis by specific interventions in the signaling pathways of tumor cells. MTT exhibits potential as an effective treatment against cancer. This approach can improve the ability to kill cancer cells and reduce damage to normal tissues. MTT has gradually been used clinically in treating cancer and shown advantages over traditional methods. This paper reviews research progress in this targeted therapy to treat gynecological cancer.

Objective To evaluate the effect of anisodamine on expression of Toll-like receptor 4 (TLR4) in the kidneys of exhausted rats.Methods Forty-eight male SD rats weighing 200-220 g were randomly divided into 3 groups:control group (group Ⅰ ) (.n =8) ; exhaustion group (group Ⅱ ) ( n =24) and anisodamine group (gronp Ⅲ) (n =16).In groups Ⅱ and Ⅲ exhaustion was induced by exhausting swimming and defined by (1) the animal sank to the bottom and stayed for＞ 10 s during swimming,(2) uncoordinated swimming,and (3) the animal lost escape/righting reflex when placed on the table In group Ⅲ intraperitoneal anisodamine 10 mg/kg was administered at 20 min before swimming.The animals were sacrificed immediately (T1 ),6 h (T2 ) and 24 h (T3 ) after exhaustion respectively.Their kidneys were obtained for detection of apoptosis and determination of TLR4 protein (by immuno-histochemical staining) and mRNA (by RT-PCR).Results Exhaustion significantly increased the number of apoptotic cells and up-regulated TLR4 protein and mRNA expression in group Ⅱ as compared with group Ⅰ,and anisodamine significantly attenuated the exhaustion-induced increase in the number of apoptotic cells and up-regulation of TLR4 protein and mRNA expression in group Ⅲ as compared with group Ⅱ.Conclusion Anisodamine can decrease apoptosis in renal cells in exhausted rats bydown-regulating TLR4 expression.

Objective To explore the microsurgery technique and outcome of expanding translabyrinthine ap-proach resection large acoustic neuroma .Methods We have retrospectively studied clinical data and follow -up re-sults of 4 patients of large acoustic neuroma (≥5 cm ,the largest was 8 cm ;3 cases with servere sensorineualt hear-ing loss ,1 case with high frequenoy hearing loss ;1 case with blindness) in our hospital between January 2013 and April 2013 .All patients received expanding translabyrinthine approach microsurgery to resecting the tumors .Post-operative follow -up was 6~10 months .Results The large acoustic neuroma of 4 cases were totally removed via MRI reexamination after operation .Facial nerve in 3 cases were dissection retained ,and 1 case received facial nerve anastomosis .Six months after operatin ,one patient’s facial nerve function recovered from level Ⅲ to level Ⅱ ,the patient underwent facial nerve anastomosis with facial paralysis level Ⅵ recovered to level Ⅳ ,and the vision of pa-tient with blindness completely recovered to normal .Conclusion The expanding translabyrinthine approach can to-tally resect large acoustic neuroma and preserve facial nerve function .

Objective To observe the effect of treating DPN with xuesaitong tablet and mecobalamine. Methods 112 patients with DPN were randomly recruited into a treatment group (60 cases) and a control group (52 cases). The control group was treated with mecobalamine, 0.5 mg. Rid., while the treatment group was treated with Xuesaitong tablet, 50mg tid.,On the basis of the control group. Both groups were treated for successive three months. Results The total effective rate was 78.33% and 57.70% in the treatment group and the control group respectively, showing a statistical difference (t= 1.9804, P=0.0271). Conclusion The combined therapy of xuesaitong tablet and mecobalamine was more effective on DPN than mecobalamine used only.