OBJECTIVE:To study compatible stability of pazufloxacin mesilate for injection with three kinds of hemostatics in sodium chloride injection.METHODS:The changes of the mixtures in appearance,pH values,insoluble particles and the content of pazufloxacin mesilate within 8 hours after mixing of pazufloxacin injection with etamsylate,Vitamin K1 or aminomethylbenzoic at 25 ℃ under natural illumination in a simulated clinical common concentration were followed.RESULTS:There were no significant change in the appearance,pH value and the content of pazufloxacin mesilate within 8 hours after mixing of pazufloxacin mesilate for injection with aminomethylbenzoic injection or etamsylate injection;when mixed with Vitamin K1,there were no significant change in the appearance,pH and the content of pazufloxacin mesilate within 2 hours,but the insoluble particles was found to be unqualified after 1 hour of mixing,and white precipitate appeared after 4 hours and the content of pazufloxacin mesilate decreased to some degree.CONCLUSION:The mixture of pazufloxacin mesilate for injection and etamsylate or aminomethylbenzoic was stable within 8 hours at room temperature.The mixing of pazufloxacin mesilate for injection with Vitamin K1 should be conducted with caution.

Child ; Epilepsies, Partial ; Humans

Inducible costimulatory molecule(ICOS)is a member of the CD28 family,which can be expressed on the tumor tissues and immune cells in the tumor microenvironment. ICOS enhances its anti-tumor activity through participating in CD4 + T and CD8 + T cell immune response and enhancing the secretion of cyto-kines on the activated T cells and NK cells. While the curative effect of cytotoxic T lymphocyte-associated anti-gen-4(CTLA-4)monoclonal antibody is relevant with CD4 + T cells expressing ICOS,which suggesting ICOS may become a novel anti-tumor therapeutic target in the future.

Objective: To introduce the Raman spectrum, the optical tweezers physical principle and its working characteristics, unify the detail medical research work, summarize specific application situation of the Raman tweezers technology in the different cells domain. Methods: Use the optical tweezers to fix the living cells, simultaneously carry out Raman spectrometry on the living cell or the cell organ by using the laser Raman technology. By applying this technology, the samples will be captured in the suspending liquid. In an approximate physiological state, the single living specimens, such as the cells, the cell organs or the biological macro-molecules, will be studied and the real-time track to the research object physiological biochemistry process will be carried on, then the Raman spectrometry will be implemented to the living cells. Results: From the single cell level, Raman tweezers technology analyzes the oxygen ability and the deformability of red blood cells of normal persons and the Mediterranean Sea anemia patients, and implements the appraisal of blood red cell and the blood platelet of different species. The Raman tweezers technology reveals the differences between the organizational structure of the cancer cells and that of normal cells in the molecular level, providing important information and data for the cancer diagnosis and the mechanism analysis. The Raman tweezers technology has implemented the torsion and knotting of DNA molecules, and realizes the control and differentiation of human being's chromosome. Conclusion: The Raman tweezers technology is the prompt and effective tool for the real-time research of cell physiology and biochemistry changes, hopefully in the molecular level. It will become one of the most advanced tools to carry out examination and diagnosis of different kind of living cell. Surely it has a very bright prospect.

Objective To study the expression of Ephrin‐B4 receptor (EphB4) in hepatocellular carcinoma (HCC) and its clinical significance ,and to analyze the effect of EphB4 on the proliferation of HCC cells .Methods The expression level of EphB4 in HCC tissues and matched paracancerous liver tissues of 60 cases of HCC patients was assessed by reverse transcriptase‐polymer‐ase chain reaction (RT‐PCR) and immunohistochemistry .The correlation between the expression of EphB4 in HCC tissues and clin‐ical pathologic parameters was analyzed by chi‐square test .Univariate survival analysis was carried out by Kaplan‐Meier Log‐rank test .The effect of EphB4 on the viability of HCC cells was furtherly analyzed by MTS .Results The results of RT‐PCR showed that the mRNA level of EphB4 in HCC tissues (1 .39 ± 0 .80) was significantly higher than in matched paracancerous liver tissues (0 .56 ± 0 .33) ,the difference was statistically significant (P< 0 .01) .Data from immunohistochemistry showed that the positive rates of EphB4 protein in HCC tissues and matched paracancerous liver tissues were 81 .7% and 23 .3% ,respectively .Moreover ,the expression of EphB4 in HCC tissues was relevant to AFP level ,tumor size and TNM stage(P<0 .05) .The three‐year survival rate of HCC patients with positive expression of EphB4 protein was 22 .5% ,and that of HCC patients with negative expression of EphB4 protein was 54 .5% ,the difference was statistically significant (P<0 .05) .Overexpression of EphB4 significantly enhanced the ability of hepatocellular carcinoma cell proliferation (P<0 .05) .Conclusion EphB4 expression was significantly up‐regulated in HCC ,which was associated with HCC progression and prognosis ,and EphB4 could promote the proliferation of HCC cells ,which could be used as a marker of HCC progression .

Artemisinin is a traditional anti-malarial drug. In recent years, many researches indicated that artemisinin and its derivatives have obvious anti-tumor effects. Its mechanisms of anti-tumor lies in cytotoxicity, direct destruction of tumor cells due to oxidative stress reaction, inducing apoptosis, inhibiting of tumor cell proliferation, restraining tumor angiogenesis and performing immunological regulation. Compared with the traditional anti-cancer drugs, artemisinin can selectively inhibit tumor cells and manifestating no cross-resistance with traditional chemotherapeutic agents. Besides it can reverse the phenomenon of multi-drug resistant of tumor cells. Therefore, artemisinin, as an new type of anti-cancer drug, will be applied to a wide range of clinical practice.

Cationic polymer, a kind of nonviral nanometric gene vector,have attracted more and more attention because they have many advantages in terms of low toxicity,lack of specific immune response,ease of large-scale production and big load.There are also many advance in how to increase the efficience of transduction.The anthor reviewed the advance of PLL and PEI as the nonviral nanometric gene vectors.

The metabolic syndrome (MetS) is a general name for a group of metabolic risk factors for cardiovascular diseases,and its major constituents include hyperglycemia,hypertension,lipid abnormality and central obesity.Because MetS portly overlaps the risk factors of stroke,and thus it determines that MetS is closely related with stroke.No matter the interaction of all individual risk factor or multiple risk factors may affect the occurrence and development of stroke.

Histiocytosis, Langerhans-Cell ; diagnosis ; therapy ; Humans ; Practice Guidelines as Topic

Fluid Therapy ; Humans ; Rehydration Solutions ; therapeutic use