Objectives:To investigate the effect and mechanism of adventitia applied slow-releasing triptolide on proliferation and apoptosis of VSMCs of the autologous vein graft. Methods:Twenty-four healthy rabbits were made external jugular vein to common carotid artery models and then were divided into 3 equal groups at random:Blank-control group received no management on adventitia of the vein graft;F-127 pluronic gel control group received local application of 0.5 ml 20% F-127 pluronic gel containing 0.1ml DMSO on adventitia;and experimental group received local application of 0.5 ml F-127 pluronic gel containing triptolide 300?g on adventitia. Vein graft samples were harvested at 2 weeks after the operation. Histomorphologic methods were used to detect the degree of intima hyperplasia of the samples. The expressions of proliferating cell nuclear antigen(PCNA) and bcl-2 of the samples were detected by immunohistochemistry. The apoptosis of vascular smooth muscle cells(VSMC) was detected by TUNEL. Results:Two weeks after vein grafting, compared with blank-control group and F-127 control group,intima hyperplasia was markedly inhibited by applying triptolide 300?g on adventitia(P

Yifeng (TE 17), Tinghui (GB 2), Zhongzhu (TE 3) and Xiaxi (GB 43) were selected as key acupoints plus hyperbaric oxygen to treat 50 cases of sudden deafness, and the total effective rate was 98%.

Objective:To study the influence of dietary restriction on the expression of insulin signaling pathway genes in mouse adipose tissue. Method:Twenty four C57BL/6 male mice of 2 w age were subjected to either AL (ad libitum) or DR (dietary restriction, 40% from AL) randomly until sacrificed.Then the epididymis fat pads were weighed, and their insulin signaling gene expression was detected by Oligo Insulin Signaling Pathway Microarray (SuperArray), and two of the differentially expressed genes, NPY and AKT3 were validated by Real Time PCR. Results:Dietary restriction significantly reduced the weight of epididymis fat pads and 31 (20 up, 11 down regulation) of 96 insulin signaling pathway genes were differentially expressed. The results of Microarray coincided with that of Real Time PCR. Conclusion:The differential regulation on the expressions of insulin signaling pathway gene may be the important molecular basis of dietary restriction.

Drawing on recently published foreign literature, this paper comprehensively analyses the research status of medical tourism from the concept of decision-making factors and risks for tourists, as well as the impacts of medical tourism. For future research, it proposes a greater focus on medical tourists, caregiver-companions and medi-cal tourism facilitators. To promote the development of Chinese medical tourism, a development model must be con-structed, international medical services, consummate policies, and regulations must be in place, and medical tourism personnel must be trained and welcomed.

Objective To give a systematic reviews on effect of taking blood gas via cephalic artery for infants.Methods Meta-analysis were used to analyze literatures about taking blood gas via cephalic artery for infants.Results 11 studies were included.The rate of successful puncture was 87.5％～98.0％ in the intervention group and 55.3％ ～94.6％ in the control group.The rate of successful puncture via cephalic artery were higher.Conclusions Effect of taking blood gas via cephalic artery for infant was better than that via other artery.Successful rate was high,while the incidence rate of local side effects was low.

Objective To prevent data transfer or loss in application development when dealing with data exchange between Oracle database with different server's character set.Methods Environment variable is set to update Oracle client's character set so as to solve data transfer or loss caused by character set transformation.Results It was the environment variable not the register list that was reset,so Oracle databases with different server's character set were not connected at the same time.Conclusion An adaptive method for different server's character set is given to solve this problem.

Monographs of Chinese medicine into the United States Pharmacopeia and the European Pharmacopoeia is the prerequisite and foundation for the aim of Chinese medicine standards leading the international standard-set-ting. By comparative analysis of the key issues of quality standards among Chinese, American and European Phar-macopoeia, thoughts and suggestions are proposed on how to implement the construction of international quality stan-dard of traditional Chinese medicine. Meanwhile, under the pressure of the present international environment, the pa-per can also provide some reference and advices which can help to break down the difficult situation for the process of internationalization of traditional Chinese medicine.

Objective To investigate the inhibitory effect of appling slow-releasing triptolide on adventitia on the intima hyperplasia of autologous vein graft.Methods In 24 male New Zealand rabbits, external jugular vein to common carotid artery models were established, and then were divided into 3 equal groups at random: blank-control group, receiving no management on adventitia of the vein graft; F-127 control group, receiving local application of 0.5 mL of 20 % F-127 on adventitia of the vein graft; experiment group, receiving local application of 0.5 mL of 20 % F-127 containing triptolide 300?g. Vein graft specimens were harvested at 2 weeks after the operation. Histomorphologic methods were used to detect the degree of intima hyperplasia of the specimens. The expression of bcl-2 and Fas of the specimens was detected by immunohistochemistry. The apoptosis vascular smooth muscle cells (VSMC) were detected by TUNEL.Results Two weeks after vein grafting, compared to blank-control group and F-127 control group, intima hyperplasia of experiment group [intima thickness (29.9?7.6)?m, I/M 0.56?0.08] was markedly inhibited (P

OBJECTIVE:To establish the method for determination of astragaloside IV and astragalus polysaccharides in Radix Astragali injection METHODS:Astragaloside Ⅳ was determined by HPLC and astragalus polysaccharides by iodometry RESULTS:The recoveries of astragaloside Ⅳ and astragalus polysaccharides were 97 51% RSD=2 48% and 99 18% (RSD=1 91%) respectively CONCLUSION:This method is accurate,sensitive and repeatable,and can be used for quality control of the preparation

Objective To determine the role of SCN1A gene variation in the development of familial febrile seizures (FS).Methods Clinical data were collected from 8 familial FS pedigrees, and peripheral venous blood samples were collected from the probands and other available family members. All 26 coding exons and exon-intron boundaries at least 50 bases of the human SCN1A gene were amplifled by polymerase chain reaction, the products were subsequently sequenced. To novo variation, other family members were screened for the corresponding exons. Two hundred age-matched healthy children were served as normal controls. ResultsA total of 33 variations in the SCN1A gene were identifled in these families. Of these variations, one was a missense mutation; the remaining 32 variations were previously submitted as single nucleotide polymorphisms (SNPs). A c.2650G>A heterozygous missense mutation in exon 15 of the SCN1A gene found in the proband of family 4 was inherited from his father who had seizures with fever in early childhood. The c.2650G>A mutation was absent in the 400 alleles of normal controls. To the best of our knowledge, the SCN1A c.2650G>A mutation has neither been reported in the NCBI SNP database nor in the literature to date. The c.2650G>A mutation changes a glycine at amino acid 884 in the SCN1A protein to a serine (p.Gly884Ser). Protein sequence analysis showed that the p.Gly884Ser is located at a highly conserved region between the 4th and 5th transmembrane segment of the homologous domain Ⅱ of voltage-gated sodium channel 1 subunit (DIIS4-S5). ConclusionsThe pathogenesis of familial febrile seizures was related to the SCN1A variation, the mutation outside the region of the voltage sensor (S4) and ion channel pore (S5-S6) of the voltage gated sodium channelα-subunit may be an important factor to cause mild phenotype epilepsy syndrome.