Objective To observe the effect of marrow mesenchymal stem cells(MSCs)transplantation combined with Qidantongmai tablet and simple MSCs transplantation on myocardial cells apoptosis and heart function in acute myocardial ischemia rats.Method Rat MSCs were isolated by density gradient method,and then cultured and amplified to the third passage labeled with BrdU.16 SD rats were divided into two groups randomly,transplantation combined with Qidantongmai tablet group and simple transplantation group.The isochoric of suspension of Qidantongmai tablet(2 g/kg)and NS were administered to SD rats by gastrogavage respectively for 14 days.The left anterior descending coronary artery of rat was ligated to establish animal models of acute myocardial infarction.The MSCs labeled with BrdU were implanted intramyocardially into the infarct area.After 3 hours,the heart function were detected by an echocardiographic system(HP 5500 system),equipped with a 12 MHz probe.After 4 weeks,the heart function were rechecked and MVD were detected by immunofluorescence,myocardial cells apoptosis were detected by TUNNEL.Results Heart function were improved in both groups,and was more significant in MSCs transplantation combined with Qidantongmai tablet group.Apoptotic index were decreased and the MVD of ischemic zone were increased in transplantation combined with Qidantongmai tablet group than simple transplantation group.Conclusion MSCs transplantation combined with Qidantongmai tablet could improve heart function of acute myocardial ischemia rats,decrease apoptosis of myocardial cells,increase the MVD of ischemic zone,the therapeutic effect were more significant than simple MSCs transplantation.

Abstract: Objective　To analyze the serotypes, drug resistance, and molecular epidemiological characteristics of non-typhoidal Salmonella in Chaozhou City, Guangdong Province in 2022, providing pathogenic support for the effective prevention and control of foodborne diseases. Methods In 2022, human-derived non-typhoidal Salmonella strains and food-derived strains were isolated through sentinel surveillance and food risk monitoring in Chaozhou City, Guangdong Province. The strains were subjected to serotyping, drug susceptibility testing, and whole-genome sequencing analysis. Results A total of 1 257 fecal specimens were collected for diarrhea surveillance in Chaozhou City in 2022, and 58 strains of non-typhoidal Salmonella were detected, with an isolation rate of 4.61%. Among 116 foodstuffs, 2 strains of non-typhoidal Salmonella were detected, with an isolation rate of 1.72%. Early childhood was the main age group for infection (14/144, 9.72%). The highest detection rate was in June (12/123, 9.76%). The 58 human-derived non-typhoidal Salmonella strains were classified into 12 serotypes, with Salmonella typhimurium being the most prevalent (44/58, 75.86%). In terms of drug resistance, 55 strains showed resistance, with a resistance rate of 91.67%, among which ampicillin, tetracycline, chloramphenicol, and streptomycin had the highest resistance rates, 76.67%, 70.00%, 67.67%, and 60.00%, respectively. Forty-nine strains were multi-resistant, accounting for 81.67%. From the molecular characteristics standpoint, multilocus sequence typing (MLST) divided the strains into 14 sequence typing (ST), with Salmonella typhimurium ST34 being the dominant type. Whole-genome single nucleotide polymorphism (wg-SNP) evolutionary analysis revealed high genetic heterogeneity among the studied strains, with Salmonella typhimurium clustering together and showing close evolutionary relationships to clinical samples of Salmonella enteritidis. Conclusions In 2022, the non-typhoidal Salmonella strains from Chaozhou showed a diversity of serotypes, with Salmonella typhimurium being predominant and exhibiting severe multidrug resistance. Molecular typing revealed diverse evolutionary characteristics.

AIM: To investigate the detrimental effect of glucocorticoid(GC)on the retinal neurons of diabetes mellitus(DM)rats.

METHODS: The DM model was induced by intraperitoneal injection(IP)of streptozotocin in adult male rats, and the solution of RU486 was configured with dimethyl sulfoxide(DMSO). RU486 treatment group with glucocorticoid receptor antagonist RU486 and diabetic group with DMSO by intraperitoneal injection was in successful DM model. Naive rats were injected with DMSO as control group. Three months later, we detected the body weight and blood glucose and GC concentration of serum. The changes of retinal ganglion cell(RGC)density was investigated by HE staining. The expression of growth associated protein-43(GAP-43, a marker of neuronal axon regeneration)and synaptophysin(SYN, a marker of synaptic number)were semi-quantity analyzed by the optical density of immunofluorescence and Western blot.

RESULTS:Compared with the control group, the body weight and density of RGC and expression of SYN in diabetic group were significantly lower(P<0.01), the blood glucose and GC concentration of serum and expression of GAP-43 in diabetic group were significantly higher(P<0.01). Compared with the diabetic group, the density of RGC and expression of GAP-43, SYN in RU486 group were significantly higher(P<0.01).

CONCLUSION: Inhibition of GC could ameliorate the axonal degeneration of retinal neurons in diabetic rats, and loss of the number of synapses, and restore the RGC density of retina. The results suggest that long-term elevation of GC may be involved in the occurrence and development of diabetic retinopathy.

Objective To obtain the physical anthropological characteristics of head and face of Tajik adults in Aketao, and to explore their ethnic origin through cluster analysis with other populations. Methods According to Anthropometric Method, 286 Tajik adults (male 121, female 165) in Aketao, Xinjiang were recruited and analyzed. The survey included 12 observation indicators of head and face, 19 measuring indicators of head and face, 12 indexes of head and face and its classification. Results The indicators of head length, head breadth, head height, minimum frontal breadth, face breadth, bigonial breadth, nose breadth, mouth breadth, auricular height, physiognomic facial height, morphological facial height, nasal length, nasal height, physiognomic ear length, physiognomic ear breadth, zygomatic protection, nasal root height, nasal profile, nasal base of Tajik adults in Aketao had gender differences (P < 0. 05). Compared with the Tajik adults in Aketao and Taxkorgan, the type indicators of head and face of zygomatic protection, direction of eyeslits, nasal root height, nasal base, maximal diameter of nostrils, lobe types, nreadth-height index of head type, morphological facial index type, nasal index type had regional differences (P<0. 05). The cluster analysis showed that Tajik adults have unique characteristics of head and face, which were grouped separately and were more similar to Iranians. Conclusion Tajik adults in Aketao have the characteristics of head and face of the West Eurasian. The characteristics of head and face of Tajik adults in Aketao and Taxkorgan are somewhat different. From characteristics of head and face, they may be related to the Eastern Iranian tribe.

We reviewed the concept of race and its development, analyzed the chaotic application of "race" , "ethnicity" and "ethnic group" at present, put forward the importance of distinguishing these concepts and put forward specific suggestions for distinguishing them. It was also pointed out that although the boundaries of race were blurred, the differences between races were narrowing, and the concept of race was fading, the concept of race can not be abolished. In practice, these concepts were used correctly according to context. At present, the concept of race was questioned, but whether the concept of race had a biological basis or not, it cannot be used as the reasons for racism.

Objective To study the physical characteristics of body of Xibe adults in Liaoning province and to accumulate data for the studyof physical anthropology. Methods The investigation method and judging standard were refered to correlative regulations in " Anthropometric Method ". The survey included 33 measurement items, 5 indicators and 6 types, 330 Xibe adult people (male 174, female 156) were recruited. Results According to the classification of body part index, the rates of long trunk, wide chest, wide shoulder and wide pelvis were the highest in all the adults of Xibe nationality in Liaoning province, the rates of middle and sub-long legs were the highest in the males, and the rates of super short legs were the highest in the females. According to the stature type, the rates of high type were the highest in males and females. Conclusion The body physical characteristics of male adults of Xibe nationality in Liaoning are the most similar to those of male adults of Xibe nationality in Xinjiang, and the body physical characteristics of female adults of Xibe nationality in Liaoning were between those of female adults of ethnic minorities in Northwest China and Northeast China.

Objective To discuss the main reasons for the difference of sedum development between Daur adults in Inner Mongolia and Xinjiang. Methods Nine circumference values and six skin fold thickness of 544 adults of Daur nationality (304 in Xinjiang and 240 in Inner Mongolia) were investigated by random sampling. Circumferences, skin fold thickness and obesity indexes of Daur nationality in Xinjiang and Inner Mongolia were analyzed. Results There were significant differences in 8 circumference without thigh circumference, 2 skin fold thickness such as triceps skinfold, subscapularis skinfold, 3 obesity indexes such as Verv index, body fat rate, body mass index(BMI). Correspondence analysis showed that subcutaneous fat development was stronger in northern regions than in southern regions. Conclusion The Daur nationality in Inner Mongolia has more fat and less muscle than that in Xinjiang. The problems of overweight and obesity in both regions are serious. It is suggested to pay attention to exercise and diet. Low temperature,lack of physical exercise, low temperature, low economic and medical standards and unhealthy eating habits can thicken subcutaneous fat.

OBJECTIVE: To investigate the effects of Deoxygedunin on Aβ deposition, learning memory, and oxidative stress induced by D-galactose combined with AlCl in model rats with Alzheimer's disease and its possible mechanism. METHODS: Male SD rats were randomly divided into three groups (=12):control group, model group (AD) and intervention group (AD+Deo). Morris water maze test was used to detect learning/memory and cognitive function in rats.Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and malondialdehyde (MDA) contents in homogenate of hippocampus were detected by enzyme-linked immunosorbent assay (ELISA).Tau protein expression in rat cerebral cortex was detected by immunohistochemistry.Western blot was used to detect the expressions of extracellular signal regulated kinase 1(ERK1), protein kinase B (PKB) and tropomyosin-related kinase B (TrkB) on TrkB signaling pathway. RESULTS: The results of water maze test showed that D-galactose combined with AlCl induced a significant increase in the escape latency compared with the control group (<0.05).Deoxygedunin could reverse the increase of the escape latency of the model group (<0.05).On the 7th day after removal of the platform, the model group showed an increase in escape latency compared with the control group and the intervention group (<0.01), and the number of crossing platforms was declined (<0.05); The results of immunohistochemistry and ELISA showed that the expressions of Aβ and tau protein in the model group were increased significantly compared with those of the control group (<0.01).The activities of SOD and GSH-Px were decreased significantly and the content of MDA was increased significantly.Compared with the model group, Deoxygedunin could reverse the increase of the expressions of Aβ and tau protein (<0.01), the decrease of SOD and GSH-Px activities (<0.05) and the increase of the MDA content (<0.05).Western blot results showed that Deoxygedunin treatment reversed the decreased phosphorylation levels of TrkB, AKT and ERK1 in hippocampus of the model group. CONCLUSIONS Supplement of Deoxygedunin can significantly reverse Aβ deposition, oxidative stress and cognitive deficits by activating the TrkB signal transduction pathway, which suggest that Deoxygedunin may serve as a promising therapeutic candidate for attenuating AD-like pathological dysfunction induced by D-galactose combined with AlCl.
Alzheimer Disease ; chemically induced ; Animals ; Disease Models, Animal ; Galactose ; Hippocampus ; Limonins ; Male ; Maze Learning ; Rats ; Rats, Sprague-Dawley

Objective To explore the somatotype characteristics and changing rules of Tajik adults. Methods The Heath-Carter bod)' type method was used to determine the body size of 280 (124 males and 156 females) Tajik adults. Results The average body size of Tajik males and females were 4. 3-3. 1-1. 8 and 7. 0-3. 1-1. 1, respectively, and both are mesomorphic endomorphy.The ectomorphy of Tajik nationality were negatively correlated with age, female endomorphy and mesomorphy were positively correlated with age, while endomorphy and mesomorphy were not correlated with age. With increasing age, the difference in body shape between female age groups was more obvious than that of males. Conclusion The Tajik have less skeletal muscle mass, and women have developed body fat, which is different from the Tibetan people and other people in the Altaic language family.

Objective To screen and identify the hub genes closely related to cardiac hypertrophy by using bioinformaticsmethod and biological experiments. Methods The chip data related to cardiac hypertrophy in mice were downloaded from the Gene Expression Omnibus (GEO) database, and the GE02R online tool was adopted to screen for differentially expressed genes; DAVID 6.7, String 11.0 and Cytoscape 3.7. 0 softwares were used to analyze differentially expressed genes; Kunming mice were randomly divided into a normal saline group (n = 6) and an angiotensin II (Ang II) group (n = 6) to establish a cardiac hypertrophy model, the expression of hub gene in Kunming mouse model of cardiac hypertrophy induced by Ang II was detected by Real-time PCR method. Results A total of 202 common differentially expressed genes and 12 hub genes were selected; the Real-time PCR result demonstrated that decorin(Dcn), HADHA and heat shock protein (HSP) 90αA 1 were significantly down-regulated in the Angli group. Conclusion The selected hub genes can influence the development of cardiac hypertrophy in Kunming mice through extracellular matrix and transforming growth factor β(TGF-β).