Objective: To understand the status of family sex education and associated factors among preschoolers in Wuhu, providing scientific basis for child family sex education. Methods: From July to August 2020, random cluster sampling was used to select 481 children from 5 kindergartens in Wuhu. A questionnaire survey among parents of these preschoolers regarding family sex education status and its influencing factors. Results: A total of 285(59.25%) parents reported family sex education for children, and 196(40.75%) did not practice family sex education for their children. Multivariate Logistic regression analysis showed parental awareness of sex education content(OR=3.06, 95%CI=1.95-4.78), parental anxiety for child sexual assault (OR=1.82, 95%CI=1.11-2.99) were associated with higher rate of family sex education. Conclusion Family sex education among preschoolers in Wuhu should be further promoted. Sex education training towards parents might help improve children s family sex education.