Objective: To understand weekend screen time of preschool children in Nanjing, Jiangsu and to explore its association with parental factors,aiming to provide effective strategies for screen time intervention. Methods: A questionnaire survey was conducted in 6 424 parents of preschool children in 20 kindergartens selected using convenience samping method in Nanjing to understand the association of screen time during weekends and parental factors. Results: The proportion of preschool children in Nanjing with screen time ≥2 h/d was 13.01%, among which the proportion of boys was 14.61%, and girls was 11.34%. The difference was statistically significant(χ2=15.27, P<0.01). Logistic regression analysis showed that there was a positive correlation between 3.5-6 years old, boys, paternal education lower than junior high school or equal to senior high school, and no exercise habits with children’s screen time ≥2 h/d(OR=1.43, 1.42, 2.21, 1.82, 2.47, 2.05, 1.36, 1.38, 1.39, 1.52, P<0.05). Conclusion The video screen time of 3-6 year old preschool children in Nanjing is at moderate level. Parents’ exercise habits and education shows impact on screen time of preschool children, and should be given attention and support.