Objective To identify the stem cell characteristics of human diploid cells 2BS and evaluate the potential as biological function positive cells for quality detection of stem cells.Methods Flow cytometry was applied to detect the expression levels of stem cell surface markers in 2BS cells.The staining with alizarin red S,oil red O and alcian blue was used to evaluate the ability of osteogenic,adipogenic and chondrogenic differentiation,respectively.Co-culture with lymphocytes was used to observe the effects on lymphocyte proliferation.Results Human diploid cells 2BS expressed the surface markers CD73,CD 105 and CD90 of stem cells,and possessed the potential of osteogenic differentiation and inhibiting lymphocyte proliferation.Conclusion 2BS cells have several properties of stem cells and could be the positive control for surface markers detection of stem cells by flow cytometry.

IL-8 is a cytokine which belongs to the chemotactic superfamily.It is one of the major mediators in the inflammatory response.In recent years,it was found that it had multiple functions,such as chemoattractant,potent angiogenic,and so on.The cytokine is believed to play an important role in the pathogenesis of inflammation,tumor progress and immune disease.In this review,the properties of IL-8 and highlight its roles on inflammation and tumor progress were surveied.

Objective To compare differences in pathological grade,clinical classification and prognosis in patients with Henoch?Sch?nlen purpu?ra nephritis(HSPN)in each age group. Methods Totally 225 cases of patients diagnosed as HSPN and treated in the hospital were selected to ret?rospectively analyze their clinical manifestations,pathological classification and prognosis and compare the difference in pathological grade,clinical classification and prognosis by age. Results Of the concomitant symptoms of HSPN,the incidence rate of abdominal pain was the highest in pa?tients of school age. There was statistical significance in the incidence rate of gastrointestinal bleeding between each age group. Of the clinical mani?festations,proteinuria was positive correlated with the pathological type,and the difference was statistically significant(r=0.471,P<0.000 1). But there was no correlation between the count of urine red blood cells in the urine and the pathological type. There was difference in the pathological grade between each age group(H=19.194,P<0.000 1). The difference mainly showed in the pathological grade between adults,children of pre?school age and adolescents(Z=-2.702,P=0.001;Z=-3.675,P<0.000 1). There was difference in clinical classification between each age group (χ2=36.114,P<0.000 1). The difference mainly showed in clinical manifestations between adults,children of school age and adolescents(χ2=19.628,P<0.000 1;χ2=18.944,P<0.000 1). For both children and adults who had developed into chronic renal failure,the renal pathology of these patients was not significantly different. Conclusion Compared with adults,children have milder pathological types and clinical manifestations and well prognosis of HSPN,and the most important factor which affects the prognosis is pathological type and urine protein can reflect the serious?ness of pathological type.

Objective:To identify the novel intronic transcripts containing 11q13.5 HERV-W gag sequence, and explore the modulation of the transcripts on the alternative splicing of host gene PTD015. Methods:Half-nested PCR and touchdown PCR were used to amplify the target transcripts. The transcripts were cloned and sequenced. The plasmids inserted with the target transcripts were transfected into JEG3 cells and the alternative spliced mRNA levels of PTD015 were measured with real time PCR. Results:A 1 739 bp novel intronic transcript containing 755 bp 11q13.5 HERV-W gag sequence, 527 bp 5′ long terminal repeat and 457 bp sequence on the 5′-end of 11q13.5 HERV-W was identified. The intronic antisense transcripts significantly down-regulated the alternative spliced mRNA levels of PTD015. Conclusion:The intronic antisense transcripts originating from the second intron of gene PTD015 could modulate the alternative splicing of the host gene PTD015.

Objective:To determine the diagnostic performance of dynamic contrast-enhanced MRI (DCE-MRI) in patients with recurrent or residual prostate cancer after radical prostatectomy. Methods:Studies were systematically searched in the PubMed, EMBASE, Cochrane library, SCI, CBM, CNKI, VIP, WanFang, and other databases. Additional studies were manually searched using the references of the retrieved articles. The retrieved deadline time was June 6th, 2013. Studies were eligible for inclusion based on the inclusion and exclusion criteria, and the qualities of the studies were reviewed based on the QUADAS criteria. The Meta Disc 1.4 software was used for meta-analysis, and a summary receiver operating characteristic (SROC) curve was constructed. The patient-based pooled weighted estimates of the sensitivity, specificity, diagnostic odds radio, and 95%confidence interval were calculated. Results:Among the 118 eligible studies, 7 articles (12 studies) were included in the meta-analysis. The pooled weighted estimates of the sensitivity, specificity, and the area under the curve were 0.88 (95%confidence interval:0.84 and 0.91), 0.87 (95%confidence interval:0.81 and 0.92), and 0.939 1, respectively. The pooled diagnostic radio (dOR) was 50.4 (95%confidence interval:26.0 and 97.6) and Q*was 0.876 4. Conclusion:DCE-MRI has high sensitivity and specificity in the evaluation of locally recurrent or residual prostate cancer after radical prostatectomy, in which the diagnostic efficiency of DCE-MRI combined with magnetic resonance spectroscopy is much better.

Objective To study the effects of acute hypervolemic hemodilution (AHH) with hydroxyethly starch 130/0.4 on function of blood clotting in patients undergoing spinal operation. Methods Thirty patients scheduled for spinal operation were randomly divided into AHH group and control group with 15 cases each. Patients in AHH group were performed AHH by transfusing hydroxyethly starch 130/0.4 (15 ml/kg) with 25 ml/min before skin incision, to achieve expansion of 20% to 30%. Patients in control group were transfused routinely. Blood samples were taken from internal jugular vein before anesthesia induction (T0), at 1 h after anesthesia induction (T1) and termination of operation (T2) for determination of hemoglobin (Hb), hematocrit (Hct), platelet (Plt), prothrombin time(PT), partial thromboplastin time ( APTT), thrombin time( TT) and fibrinogen( FIB), at the same time heart rate( HR), mean arterial pressure (MAP) and central venous pressure (CVP) were observed and recorded. Results HR and MAP in both groups had no statistical difference within or between groups (P > 0.05). CVP at T1 and T2 in AHH group [(11.8 ± 1.0,(11.3 ± 1.0) cm H2O (1 cm H2O = 0.098 kPa)]were higher than that at T0 [(6.3 ±0.7) cm H2O]and that in control group ( P < 0.05). Hb and Hct at T1 and T2 in AHH group were lower than that at T0 and that in control group (P< 0.05). Plt and FIB at T1 and T2 in AHH group decreased significantly than that at T0(P<0.05), while there were no statistical difference between two groups(P> 0.05). PT, TT and APTT in both groups had no statistical difference within or between groups (P > 0.05). Conclusion AHH with hydroxyethly starch 130/0.4 has no significant effect on function of blood clotting in patients undergoing spinal operation.

Objective To analyze the characteristic of articles, authors and citations in Journal of Clincal Neurology from 2008 to 2015.Methods The data about articles, authors and citations in Journal of Clincal Neurology from 2008 to 2015 were analyzed by bibliometrics method.Results There were 1623 papers published in Journal of Clincal Neurology during the 2008 to 2015 years.The average number of articles per issue was 33.8.The average number of article per paper was 0.424.The papers with funded projects were 432 (26.6%).The first authors were mainly come from Jiangsu, Shandong, Beijing, Shanghai and Hebei, etc.The average cooperation degree was 4.17, and the average co-author rate was 95.81%.The average rate of the papers with citation was 93.96%, and the total number of citations was 18708.The citations were mainly come from foreign language (74.3%) and journals (97.7%).The average Price index was 37.72%.Conclusion Journal of Clincal Neurology is an academic journal of high quality, with strong influence in field of clinical neurology.

Extramural vascular invasion(EMVI)was defined as the presence of tumor cells within an endotheliumlined vessel beyond the muscularis propria.High-resolution MRI had 100％sensitivity and 89％specificity in identification of large(caliber greater than 3 mm)EMVI with histopathology as reference.EMVI detected by both histopathology and MRI was significantly associated with local recurrence,distance metastasis and tumor-related death in rectal cancer,which was also identified as an independent prognostic factor for 3-year tumor-free survival and overall survival.EMVI could be used as one of the risk factors,which would allow stratification of patients with rectal cancer to receive neoadjuvant chemoradiotherapy(neoCRT).Moreover,EMVI detected by high-resolution MRI was identified as an independent predictor for response to neoCRT.Tumor regression grading scale defined by high-resolution MRI could be used to evaluate EMVI regression after neoCRT.

Objective To explore the clinical application of two endometrial mechanical stimulation methods in patients with repeated implantation failure (RIF) in frozen-thawed embryo transfer (FET) cycle. Methods A total of 127 women with RIF who planned to perform FET again were included. They were separated into 3 groups: Group A (45 cases) with endometrial scratching and group B (42 cycles) with endometrial sucking within menstrual period in FET cycle, while in control group C (40 cases), no endometrial mechanical stimulation was performed. The outcomes of pregnancy were compared. Results The endometrial types of endometrial scratching group were mainly type A. The embryo implantation rate and clinical pregnancy rate in group A (27.27%, 46.67%) were significantly higher than those in group B (12.75 %, 21.43%) and group C (11.46%, 20.0%) (P <0.05). Conclusion The endometrial mechanical stimulation can improve the endometrial receptivity, compare with endometrial sucking, endometrial scratching can improve the clinical pregnancy rate of RIF patients more obviously.