Drugs, Chinese Herbal ; pharmacology ; Embryo Implantation ; drug effects ; Endometrium ; drug effects ; physiology ; Female ; Humans

Objective To evaluate the effect and mechanism of HMG-CoA reductase inhibitor simvastatin on experimental interstitial fibrosis. Methods Experiments on rat 5/6 nephrectomy chronic renal failure model and primary cultured renal interstitial fibroblast cells were conducted in this study. The cell proliferation, extracellular matrix, c-fos mRNA expression of rat interstitial fibroblasts were measured by MTT assay, immunohistochernitry, semi-quantitative reverse-transcript PCR methods, respectively. Results Serum cholesterol, triglyceride and creatinine of treated group were significantly reduced by simvastatin as compared with controls. No statistical significance in BUN was observed between untreated and simvastatin-treated rats. Histological examination revealed that simvastatin caused a reduction in the glomeruli with sclerosis. Tubulointerstitial injury paralleled the degree of glomerular damage. Simvastatin in a dose-dependent manner inhibited the proliferation of renal intersititial fibroblasts, decreased the secretion of lamimn( LN), and suppressed the expression of c-fos mRNA, as compared with normal controls. No obvious effect on hyaluronic acid( HA) secretion of fibroblasts was found. Conclusions Simvastatin is anti-proliferative in interstitial fibroblasts and decreases the secretion of laminin. This effect is exerted, at least in part, via inhibition of the c-fos and c-jun-dependent mitogenic pathway. Simvastatin may prevent interstitial fibrosis development and attenuate renal damage in uremic rats with hvperlipidemia.

Objective: To study the chemical constituents from the ethyl acetate fraction of Ceriops tagal. Methods: Compounds were isolated and purified by various column chromatography, recrystallization, and HPLC. And their structures were elucidated by physicochemical property and spectral analysis. Results: Eight compounds were isolated from the ethyl acetate fraction of Ceriops tagal and identified as (E)-methyl-3-(5-hydroxy-2,3,4-trimethoxyphenyl)acrylate (1), tagalphenylpropinoidin A (2), coniferyl aldehyde (3), 2,3-dimethoxy-5-(1-propenyl)phenol (4), methyl syringate (5), syringaldehyde (6), pinosylvin monomethyl ether (7), and n-hexadecane acid (8), respectively. Conclusion: Compound 1 is a new phenylpropanoid, named as tagalphenylpropanoidin C, and compounds 4-8 are obtained from C. tagal for the first time.

Humans ; Infant ; Male ; Mycobacterium tuberculosis ; Otitis Media ; microbiology ; Tuberculosis

Adult ; Female ; Genotype ; Humans ; Male ; Middle Aged ; Phenotype ; Thrombophilia ; genetics ; Thrombosis ; Young Adult

Objective: To study the potential mechanism of Linggui Zhugan Decoction in the treatment of Alzheimer' s disease (AD) by using network pharmacological technology. Methods: Firstly, TCMSP, Swiss, SuperPred, Stitch databases and supplement literatures were combined to search for the chemical compounds and the potential targets related to the four herbs in Linggui Zhugan Decoction, using the oral bioavailability (OB) ≥ 30%, drug-like (DL) ≥ 0.18 as screening conditions. Meanwhile, the targets related to AD were screened through OMIM, TTD, GAD and Pharm Gkb databases. Moreover, the interactive targets of Linggui Zhugan Decoction and AD were further acquired. Then, Gene Ontology (GO) analysis and KEGG metabolic pathway enrichment analysis of interactive targets were performed using DAVID 6.8 database. Finally, degree and betweenness centrality analysis were performed to find potential key targets and key pathways after the "Herbs-Active Compounds-Targets" interaction network was constructed by Cytoscape software. Results: There were 126 potential active compounds and 120 potential interactive targets in Linggui Zhugan Decoction. A total of 565 enrichment results were obtained by GO analysis, including 396 biological processes, 121 molecular functions and 48 cell components, mainly involving protein binding, cell, oxidation-reduction process, etc. Simultaneously, the selected targets were enriched by KEGG analysis and it indicated that 67 metabolic pathways play an important role in AD, primarily including neuroactive ligand-receptor interaction, pathways in cancer, serotonergic synapse and calcium signaling pathway, etc. Conclusion: The synergetic effect of Linggui Zhugan Decoction with multi-compounds, multi-targets and multi-pathways in the treatment of AD were revealed by network pharmacology, which provides a theoretical basis for the study of its pharmacodynamic material basis and action mechanism.

By functional plates,16 strains which can produce?-mannana-se were isolated frnm 28 Bacillus spp.Using a pair of degenerated primers,the conserved fragments of?-mannanase gene from the selected strains were amplified by PCR.The obtained nucleotide fragments were sequenced and compared with the homologous?-mannanase genes in GenBank and a phylogenetic tree was generated.Comparing to the genes coding?-mannanase published,the cloned nucleotide fragments show the highest sequence identity between 62% and 98%.The genes coding fnr?-mannanase of Bacillus circulus have low identity while the?-mannanase genes of Bacillus subtilis and other Bacillus spp. have high identity.

OBJECTIVETo study the relationship between pharmacological effects and properties of traditional Chinese medicine by the decision tree algorithm.

METHODBased on of pharmacological effects of traditional Chinese medicine, the decision tree algorithm was applied in the study on the relationship between pharmacological effects and properties of traditional Chinese medicines. A model was established with the decision tree algorithm for the purpose of predicting the properties of traditional Chinese medicine components.

RESULTThe established model was reliable and stable, and could be used to predict the properties of traditional Chinese medicine components.

CONCLUSIONThe prediction for the properties of traditional Chinese medicine components with a decision tree model could reflect the theoretical connotation of the properties of traditional Chinese medicine components to some extent and provide a new method for studying the properties of traditional Chinese medicine components.

Adenocarcinoma, Mucinous ; metabolism ; pathology ; surgery ; Carcinoid Tumor ; metabolism ; pathology ; surgery ; Chromogranin A ; metabolism ; Diagnosis, Differential ; Gastrectomy ; methods ; Gastrointestinal Stromal Tumors ; pathology ; Humans ; Male ; Middle Aged ; Neoplasms, Multiple Primary ; metabolism ; pathology ; surgery ; Stomach Neoplasms ; metabolism ; pathology ; surgery ; Synaptophysin ; metabolism

Objective To investigate the effect of hyperacute intensive antihypertensive treatment on the prognosis of intracerebral hemorrhage in basal ganglia region. Methods From January 2013 to December 2015,100 patients with intracerebral hematoma in basal ganglia region (onset ≤3 h)at the Neurological Intensive Care Unit,the First Affiliated Hospital of Dalian Medical University were enrolled prospectively. They all randomly received the intensive antihypertensive or standard antihypertensive treatment voluntarily. They were divided into either an intensive antihypertensive group or a standard antihypertensive group according to the random number table (n = 50 in each group). Within 1 h after beginning to treatment,the target systolic blood pressure was controlled in 130 -140 mmHg in the intensive antihypertensive group,the target systolic blood pressure was controlled in 160 -180 mmHg in the standard antihypertensive group,and the target systolic blood pressure was maintained respectively in the following 7 d. Head CT was performed gain at 24 h after treatment. The intracranial hematoma expansion was evaluated. The National Institutes of Health Stroke Scale (NIHSS)and the modified Rankin scale (mRS)were used to
evaluate their prognoses. The differences of the cumulative mortality in both groups were compared at the same time. Results The incidences of hematoma expansion of the intensive antihypertensive group and the standard antihypertensive group were 12. 0% (6/ 50)and 30. 0% (15/ 50)respectively. There was significant difference between the 2 groups (χ2 = 4. 882,P = 0. 027). There were no significant differences in NIHSS scores within or between both groups at each time points (all P > 0. 05). They were followed up for 90 d,no adverse events occurred in both groups. The favorable prognosis rates of the neurological function were 36. 0% (18 / 50)and 18. 0% (9 / 50)respectively in the intensive antihypertensive group and the standard antihypertensive group. There was significant difference between the 2 groups (χ2 = 0. 411,P =0. 043). Kaplan-Meier curves showed that the cumulative mortality at 24 h,within 7 d and 90 d in the intensive antihypertensive group and the standard antihypertensive group were 4. 0% (2 / 50),6. 0%(3 / 50),and 10. 0% (5 / 50),respectively,those of the standard antihypertensive group were 10. 0%(5 / 50),24. 0%(12 / 50),and 30. 0%(15 / 50),respectively. The results of Log-rank test found that there was significant difference in cumulative mortality between the 2 groups (χ2 =6.280,P =0.012). Conclusions The intensive antihypertensive treatment in the hyperacute cerebral hemorrhage is safe and feasible in basal ganglia region. It contributes to improve prognosis of neurological function,and reduce the incidence of hematoma expansion and the 90 d cumulative mortality.