Objective To establish a better and easier method for producing resveratrol and soybean isoflavone from polydatin and genistin by microbic metabolic enzyme. Methods The fungal strain Aspergillus niger S was fermented with solid medium. The enzyme isolated from Aspergillus niger S was purified by precipitation with ammonium sulfate and column chromatography with Q sepharose fast flow and superdex G200. The purified enzyme could be used to transform both resveratrol and soybean isoflavone. An analytical method for all the transformed products was established by the improved RP-HPLC. The HPLC separation was performed using a C18 250mm?4.6mm column and a gradient elution program. The eluent was acetonitrile/0.1% acetic acid in a flow rate of 1ml/min. The wavelength for ultraviolet detection was 280nm. Results The molecular weight of the enzyme isolated from Aspergillus niger S was about 165 kD, which was quite different from other reported glucosidases. The optimal temperature for this enzyme was 50℃, and the enzymatic activity decreased when the temperature dropped below 40℃ or elevated over 60℃. The optimal pH values were 5-10, and the enzyme would lose its activity if the pH was below 4. It was revealed by improved RP-HPLC that the products were polydatin and genistin at 27min and 39min, respectively, before enzyme treatment, while the transformed products at 45min and 64min after the enzyme treatment were resveratrol and genistein, respectively. Conclusions This purified enzyme isolated from Aspergillus niger S can transform both resveratrol and soybean isoflavone from low active form to high active form. It is a convenient and accurate method for the determination of resveratrol and soybean isoflavone. This method may be very useful in industry as well as clinical experimentation.

Immunoglobulin A nephropathy (IgAN),characterized pathologically by deposition of IgA complexes in the glomerular mesangium,is the most common form of primary glomerulo-nephritis in children and adolescents and is considered to be one of the important cause of end-stage renal failure.Abnormal immune factors become the research hotspot in recent years,but the specific pathogenesis has not yet been fully elucidated.Toll-like receptors are a family of receptors,which have been evolutionarily conserved to recognize pathogen-associated molecular patterns.Toll-like receptors do not only play an important role in activation of innate immune,but also regulate acquired immune,which is a bridge connecting the natural immune and acquired immune.Studies have shown that Toll-like receptors plays an important role in the process of occurrence and development of IgAN.This paper reviews its biological characteristics and the pathogenesis of IgA nephropathy.

Congenital cataract accounts for 10％ of childhood blindness worldwide and is the second leading cause of childhood blindness in China.30％-50％ of congenital cataract patients are caused by heritability,and majority of them is autosomal dominant traits.In addition,autosomal recessive and X-linked fashion also are found to lead to congenital cataract.Up to now,more than 20 disease-causing genes and 100 mutation locus have been mapped to different chromosomal locations in the human genome using genetic linkage methods.The cataract-associated genes were classified as four categories,including crystallin gene,membrane protein gene,transcription factor regulatory gene and others,and they induce different phenotypes of cataract by different molecular mechanisms.To understand the disease-causing genes and their mechanism is helpful for the genetic diagnosis and treatment of congenital cataract.The current researching progress in molecular genetics of cataract is summarized in this review.

Objective To discuss the relationship between the incidence of lymph node metastasis and tumor size in patients with non small cell lung cancer,and to provide evidence for clinical operation and follow -up treat-ment.Methods 180 patients with non small cell lung cancer who were hospitalized and recieived Lobectomy opera-tion were selected,the clinical data of patients and resection of tumor size,lymph node metastasis were retrospectively analyzed,and then the relationship between the lymph node metastasis and tumor volume was investigated.Results 180 cases of patients with lymph node metastasis rate was 37.20%,in which hilar lymph node metastasis rate was 11.02 percent and mediastinal lymph node metastasis rate was 10.95%.Lung lymph node metastasis rates in lymph node metastasis,hilar and mediastinal lymph node metastasis of T1a were 13.21%,15.09% and 13.21%;those of T1b period were 38.09%,35.71% and 28.57%;of T2a period were 44.44%,37.78% and 31.11%;of T2b period were 64.26%,57.14% and 53.57%;and those of T3 period were 58.33%,66.67% and41.67%.Lymph node metastasis,hilar and mediastinal lymph node metastasis in lung lymph nodes transfer rate with patients of T1a had significantly differences compared with patients of T1b,T2a,T2b and T3 stage.(χ2 =5.363,6.175,4.372,8.778, 5.363,9.631,7.936,9.631,20.463,7.521,6.175,4.387,all P <0.05).Hilar lymph node metastasis and lung metastases of T1b and T2b had a significant difference (χ2 =12.353,4.585,all P <0.05).Conclusion For non -small cell lung cancer,tumor size is positively correlated with lymph node metastasis and the greater the tumor size, the greater the likelihood of lymph node metastasis.

BACKGROUND: The nano-hydroxyapatite (NHA) biomaterials compounded with secondary phase or multiphase materials have been widely used in the domain of stomatology, and there have been several reports regarding the application of compounded NHA with composite resin. OBJECTIVE: To analyze the application progress of NHA in the domain of stomatology, and to prospect the potential significance of NHA in the stomatology materials. METHODS: Databases of Pubmed, EBSCOhost and China Journal Full-text were retrieved to screen out the articles. Inclusion crteria: ①The articles possess originality and dependable point and evidence. ②The articles have conclusive viewpoint and allround analyses. ③The agents of articles contact with this review closely.Exclusion criteria:repetition research and independent literatures with this review. And 24 literatures in accordance with the standard were selected in this study. RESULTS AND CONCLUSION: Many clinical application and animal experiments indicate that NHA has been extensively used in the oral surgery, endodontic, implantation and the other aspects, with the deeply research for combination of NHA and composite resin, NHA hopefully displays greater effectiveness in the stomatology materials.

Only realizing part of work in fixed assets management department, hospital account of fixed assets management system is unable to integrate the equipment using,management and finance departments. While the bar code management system can merge three departments into one system and manage the fixed assets of hospital with consistency of accounts, card and equipment.

Objective To study the diagnosis and treatment of acute gangrenous cholecystitis in senile (patients), so as to decrease complications and lower the mortality rate. Methods The clinical data of 112 senile patients with acute gangrenous cholecystitis were retrospectived analysed.Results 19 cases(17.0%) of the 112 patients had hyperpyrexia, 8(7.1%) had icterus,11(17.0%) had a mass in the right upper abdomen, 26(23.2%) had symptoms of peritonitis; 11(9.8%) were complicated with acute edematous pancreatitis, 5(4.5%) had acute obstructive suppurative cholangitis, 6(5.4%) had septic shock. All of the 112 patients underwent surgery. At operation, 79 of 95 cases of acute calculous cholecystitis had (gangrene) of gallbladder,16(16.8%) had gangrene with perforation; in 17 cases with acute non-calculous cholecystitis had gangrene of gallbladder, 9(52.9%) had gangrene with perforation. Of the 112 patients, 86 underwent cholecystectomy, 18 underwent partial cholecystectomy, 5 underwent cholecystectomy with bile duct exploration and T tube drainage, and 3 underwent cholecystostomy 104 patients(92.9%) were cured, 8 patients(7.1%) died, and 9(8.7%) of the 104 cured patients had postoperative complications. (Conclusions) Acute gangrenous cholecystitis in senile patients should be definitely diagnosed as soon as (possible), co-existent diseases should be correctly treated, emergency operation should be performed with in 24 hours of onset of symptoms(or in the shortest time after admission), and a suitable operation should be (selected).

Objective We generated an experimental canine model of heterogeneous emphysema.The dogs subsequently underwent unilateral bronchoscopic lung volume reduction(BLVR).Observing the postoperative condition of ventilation/perfusion,blood gas analysis,respiratory dynamics,hemodynamic measurement,HRCT and radiologic outcomes,compared with the preoperative level,the correlative mechanism and the effects of BLVR were analyzed.Methods There were 15 healthy dogs that were treated samely with localized papain instillations under bronchoscopic guidance to generate heterogeneous emphysema.The right dorsal lobe was selected as the target area.All dogs were divided into 3 groups randomly.Group A was control group;Group B and Group C received BLVR 6 weeks later while group A was raised as the same way.Group B underwent endobronchial valve insertion(EVI);Group C underwent bronchial blocking with albumin gel.Measurements were made in each animal at 3 time points:prior to papain exposure(base-line),after establishment of emphysema(6 weeks later),6 weeks after BLVR.Data included blood gas analysis(PaO2,PaCO2),respiratory dynamics(respiratory peak pressure,lung compliance),hemodynamic measurement(pulmonary artery pressure,pulmonary capillary vessel wedge pressure),nuclear ventilation/perfusion scan(CTS,CTS/PIX).Dogs were euthanized at 6-week time point followed by autopsy.The data was statistically managed and compared.Results After development of emphysema,all dogs exhibitted aggravation in PaO2,PaCO2,PAP and lung compliance(P0.05).Through ventilation/perfusion scan,CTS/PIX of the target areas reduced(P

AIM: To study whether there was a correlation between central foveal thickness (CFT) assessed with optical coherence tomography (OCT) and visual acuity of patient with high myopia after phacoemulsification and intraocular lens implantation.METHODS: Totally 67 patients with high myopia underwent phacoemulsification and intraocular lens implantation were enrolled in the study.Best corrected visual acuity (BCVA) was recorded and CFT was measured using OCT at 1wk,1 and 3mo after oerations.BCVA and CFT were compared before and after the operation.All patents were divided into two groups by the BCVA at 3mo after operation,BCVA>0.5 in Group A and BCVA≤0.5 in Group B.ANOVA,Spearman correlation analysis and independent t test were used for statistical analysis.RESULTS: There was a statistically significant difference between preoperative BCVA and postoperative BCVA (F=115.04,P<0.01).Preoperative CFT was different compared with that 1wk and 1mo after operation (P=0.04,0.02) and was not different with that 3mo after operation(P=0.52).There was a statistically significant difference in CFT of postoperative 3-month compared with that of postoperative 1-week(P<0.01) or that of postoperative 1-month (P<0.01).BCVA showed significant positive correlation with CFT without foveal lesion on postoperative 3mo (r=0.28,P=0.03).CFT of Group A and Group B was significantly different at 3mo after the operation (t=-2.24,P=0.03).There was no significant difference in age and intraocular lens of two groups.CONCLUSION: Optical coherence tomography allow for objective assessment of retinal construction changes in eyes with high myopia are correlated to visual acuity.