Objective: This paper constructed the children’s multiple sports activity module from the perspective of Multiple Intelligences, and explored the effect of sports activity module on physical fitness of young children. Methods: A total of 176 children aged 3-6 years were randomly divided into control and experimental classes for 24 weeks, 5 times/week, 30 minutes/time. Children in the experimental class received multi-sports activity module, and children in the control class received general sports activities. Activity duration, intensity and frequency of the experimental class and the control class were basically the same. Results: This study constructed the children’s multiple sports activity module with eight pieces of content from the perspective of Multiple Intelligences. Before the intervention, there was no statistical difference between the experimental class and the control class(P>0.05), and the height of the children aged 3 to 4 years after the intervention in the experimental class [(109.29±6.56)cm] was significantly higher than that before the intervention[(105.11±5.51)cm](P<0.01). After intervention, the walking balance beam, the 10-meter reverse run and the feet continuous jump of the children aged 3 to 4 years in the experimental class were significantly higher than those in the control class(P<0.01). The standing long jump, sitting position forward flexion of the children aged 3 to 4 years were also significantly higher than those in the control class(P<0.05). The balance beam of the children aged 4 to 5 years in the experimental class was significantly higher than that in the control class(P<0.01). Tennis throw, sitting position forward flexion, 10-meter reverse run, feet continuous jump of the children aged 4 to 5 years were also significantly higher than the control class(P<0.05). The walking balance beam of the children aged 5 to 6 years was significantly higher in the experimental class than that in the control class(P<0.01), the standing long jump tennis throw, the sitting position forward flexion, The 10-meter reverse run and the continuous jump of the feet of the children aged 5 to 6 years were also significantly higher than those in the control group(P<0.05). Conclusion The multi-sports activity module is superior to the general sports activities in developing children’s physical fitness and can be promoted as a project to develop children’s physical fitness.