Objective: To understand the current status and influencing factors of anti-drug ability of college students in Beijing, and provide suggestions for drug education in universities. Methods: A total of 966 students from 11 universities in Beijing were investigated with a self-designed questionnaire. and the data was analyzed with quantitative statistical analysis method. Results: The average types of drug college students are aware of was 6.64. Among the average cognitive drugs of college students in Beijing,the full score rate of drug resistance was 68.12%. Gender, different majors, and frequency of receiving drug prevention education were influential factors of drug knowledge and anti-drug ability of college students (χ 2=41.38，18.20，11.02，P<0.05). The commonest educational method was themed lectures (80.33%), and the Internet had played an increasingly important role in education (76.60%). Anti-drug short-movies was the most interesting education method for college students (79.30%), with a penetration rate of 42.44%. The number of drugs awared and the ability of anti-drugs improved significantly through the "6.27" project. Conclusion The anti-drug propaganda and education should be strengthened among the students majoring in sport arts, and the function of anti-drug videos should be greatly exploited to maximize the effect of education.