Objective: To understand participation and attitude towards online public opinion on gender issues among college students. Methods: Using convenient sampling, 450 valid questionnaires were collected from 9 colleges and universities in Xi an, Shaanxi Province, and self compiled questionnaires were used to investigate general demographic information, public opinion concern and participation of gender issues, case attitudes and the impact of online public opinion of gender issues. Results: A total of 371 participants (83.00%) paid attention to such events, and 210 (47.00%) participated in the discussion. Among the 326( 72.90 %) college students who were affected by such events, 62(13.90%) thought that such events had a lasting effect on self emotion, and 54(12.10%) thought that such events had further effects on self emotion, affect their own lives. Attention to and participation in the event(4.58±2.94), positive evaluations of victims(12.01±2.07), negative evaluations of perpetrators(5.99±1.60), and negative emotions(15.90±8.62) in female college students were significantly higher than those of males(3.85±2.67, 11.44± 2.02 , 6.56±1.83, 9.46±9.02)( t=2.76, 2.96, 3.48, 7.71, P <0.05). Conclusion College students have a high degree of attention to social public opinion events on gender issues, with significant gender difference. Schools, media and society should pay attention to the media literacy and mental health among college students.