[Objective] The paper tries to find out the ways and methods for cultivating medical students ’ humanistic quality. [Methods]By doing questionnaire survey among students who major in clinical medicine, Chinese medicine, science of acupuncture, rehabilitation, au-diological medicine, medical images, pharmacy and so on to analyse the present situation of medical students ’ humanities in Zhejiang Chinese Medical University, then to demonstrate that strengthening the education of the humanistic quality is necessary to the success of the medical students. [Results] Survey results show that the medical students are satisfied about the education of the humanistic quality scheduled by the Zhejiang Chinese Medical University, but there are still problems in the concepts, systems, measures, and so on. In face of these problems, the paper puts forward some strategies, such as changing concepts and ideas, establishing the modern medical educa-tion, based on the first class, developing the second classroom, to let the medical humanistic spirit throughout the physical teaching and the practice teaching.[Conclusions]The ways and methods from the paper are practical and feasible. The importance and necessity of them are obvious.

OBJECTIVE:To determine the concentration of Dermatan Sulfate,a new antithrombotic medicine in blood or urine by spectrophotometry.METHODS:In Britton Robinson buffer solution(pH=5.8),color fading reaction occurred when Dermatan sulfate combined with neutral red dye to form ionic associate.The reduction of absorbance of the system was positively correlated to dermatan sulfate concentration,with the maximum absorption wavelength at 526 nm.RESULTS:The linear range of dermatan sulfate was 0.18～4.0?g?mL~(-1)(r=0.999 1) with a detection limit of 0.054?g?m~(-1).The average recovery was 100.3%(RSD=1.2%),with RSD of dermatan sulfate at low,medium,and high concentrations at 2.9%, 1.5%,and 1.1%,respectively.The coexisting substances did no interference on the determination results.CONCLUSION: The method is simple,accurate,and sensitive with good methodological selectivity and it achieved satisfactory results in the determination of dermatan sulfate in blood or urine.

OBJECTIVE:To explore the effects of Haikangling Mixture on immunological function in immunosuppressed mice induced by cyclophosphamide. METHODS: Mice were given Haikangling Mixture intragastrically for 14d, and the level of hemolysin was determined by sheep erythrocyte (SRBC) immunization to exploer the influence of Haikangling on humoral immunity in mice; splenic lymphocytes were measured by MTT to observe the influence on cell immunity; the clearance of macrophage were measured by carbon particle clearance method to explore the influence on non-specificity immunity. RESULTS: The level of hemolysin, the cell reproductive activity, splenic lymphocytes and the phagotrophy of mononuclear macrophage were improved obviously by Haikangling Mixture in immunosuppressed mice induced by cyclophosphamide. CONCLUSION: Haikangling Mixture can enhance the immunological function in immunosuppressed mice induced by cyclophosphamide.

Purpose Accurate diagnosis of triangular fibrocartilage (TFC) tear is very important for treatment. MRI is most used for diagnosing TFC tear. This paper aims to evaluate MRI and MR arthrography (MRA) for diagnosing TFC tear by meta-analysis. Materials and Methods The articles were searched in the databases such as Wanfang, VIP, CNKI, Cochrane Library, Medline, Embase and PubMed. The QUADAS items were used to evaluate the quality of the included studies. Heterogeneity of the included articles was tested. The pooled weighted sensitivity and specificity of MRI and MRA in diagnosing TFC tear were calculated, and the pooled receiver operation curve was drawn. Results Fifteen articles met the inclusion criteria, 2 were Chinese articles and 13 were English articles. The subjects and methods of the articles were different and existed heterogeneity. The sensitivity and specificity of MRI for diagnosing TFC tear were 0.66 (95% CI 0.61-0.71) and 0.75 (95% CI 0.69-0.81), and those of MRA were 0.80 (95% CI 0.73-0.87) and 0.86 (95% CI 0.74-0.93). The area under curve and Q* index of SROC of MRI were 0.8566 and 0.7875, respectively. The area under curve and Q* index of SROC of MRA were 0.9123 and 0.8446, respectively. Conclusion The accuracy of TFC tear avulsion for MRA are higher than for MRI, when there is unclear of TFC avulsion using MRI, MRA can be used for diagnosis.

Objective To explore the diagnostic value of plasma fetal DNA level in preeclampsia.Methods Thirty cases of pregnant women with preeclampsia(at 33 weeks and 3 days) and 30 cases of normal pregnant women(at 34 weeks and 3 days) were selected.All the pregnant women carried a male fetus by B-ultrasound,and were sampled at gestational 20 weeks,third trimester and at 1 hour,3 hours,6 hours after delivery.SRY levels in maternal blood were quantitated by polymerase chain reaction(QF-PCR).The endotheliotoxin(ET) level was measured with RIA.Results (1) Mean fetal DNA level of patients with preeclampsia at 20 weeks of gestation was(316?61)copy/ml.They were(266?79)copy/ml,(396?91)copy/ml,(165?43) copy/ml for light and severe preeclampsia women and normal pregnant women,respectively.Maternal blood fetal DNA levels in pregnant women with preeclampsia at 20-weeks of gestation were significantly higher than those normal pregnant women(P

Objective: To study the protective effect of Chinese materia medica of tonifying kindey and eliminating sputum on chronic bronchitis mice induced by tobacco smoking from aspect of pathomorphology changes. Methods: 60 Healthy male mice were divided into normal group, chronic bronchitism model group, Guilong Kechuanning group, high and low dosage of tonifying kindey and eliminating sputum groups at random, each 12. Model mice of chronic bronchitis were induced tobacco smoking, giving Guilong Kechuanning and Chinese materia medica of tonifying kindey and eliminating sputum at the same time. The pathomorphology changes of trache and lung tissue were observed under light microscope. Results: Compared model group, the pathomorphology changes of trache and lung tissue in Guilong Kechuanning group, high and low dosage of tonifying kindey and eliminating sputum groups relieved(P

OBJECTIVE:To establish an HPLC method for the determination of Stillbene glycoside (THPG) in Zhipingk-ang misture.METHODS:The chromatographic column was Hypersil C18(250 mm?4.6 mm,5 ?m).The mobile phase consisted of acetonitribl-water(25∶75) at a flow rate of 1.0 mL?min-1 with detection wavelength set at 320 nm. RESULTS: There was a good linear relationship within the concentration range of 0.5～8.0 ?g for THPG. The average recovery of THPG was 100.03%(RSD=1.07, n=6).CONCLUSION: The adoptd method is accurate, reproducible and suitable for the quality con-trol of Zhipingkang misture.

OBJECTIVE: To explore the feasibility and safety of analgesia fiber bronchoscopy assisted by midazolam and fentanyl citrate. METHODS: A contrastive study was performed between analgesia bronchoscopy group(group A,n=58) and conventional bronchoscopy group (group B,n=57). RESULTS: 100% of patients in group A completed the examinations safely; 3.5% of patients in group B had to stop the check due to excessive pain. Group A was better than group B in the following aspects: better anaesthetic effects that made physicians perform better targeted examination(P

AIM: To investigate the influence of GM-CSF on human vascular endothelial cells induced to form new blood vessels and the role of VEGF.METHODS: HUVECs were cultured by Matrigel to set up a stable angiogenesis system with the stimulating factors.The rhGM-CSF concentration-dependent and time-dependent effects and the role of VEGF165 were detected.CD34 was measured by immunochemical staining and numbers of vessel formation was calculated under microscopic observation.RESULTS: After treatment with rhGM-CSF at various concentrations and at different time points,the numbers of vessel formation increased in a dose-dependent and time-dependent manner.In the presence of VEGF165,the numbers of vessel formation increased evidently.CONCLUSION: HUVECs were induced to develop tubular structure in vitro cultured with Matrigel.GM-CSF promotes human vascular endothelial cells to form vessel-like structure in vitro in a dose-dependent and time-dependent manner.VEGF also in vitro promotes human vascular endothelial cells to form new vessel-like structure.

Objective To investigate causes and effects of medial meniscus extrusion in patients with knee osteoarthritis.Methods A total of 120 patients diagnosed as knee osteoarthritis between January 2011 and March 2012 were enrolled in this study,including 60 patients with medial meniscal extrusion confirmed by MRI (extrusion group) and other 60 patients without medial meniscal extrusion (control group).The extrusion distance of medial meniscus and tibiofemoral angle were measured on MRI.The correlation between tibiofemoral angle and extrusion distance were analyzed.The incidences of genu varum,medial meniscus injury and cartilage lesion of medial tibiofemoral joint were compared between two groups.The effect of medial meniscal extrusion on meniscus injury and effect of genu varum on meniscal extrusion were analyzed.Results In extrusion group,the extrusion distances of medial menisci ranged from 3.76 to 11.6 mm (average,8.3±1.79 mm); all patients had genu varum,and the tibiofemoral angle ranged from 174°to 181°(average,179.0°±2.2°); the incidence of medial meniscus injury was 50.0％ (30/60) in the anterior horn,93.3％ (56/60) in the body,and 93.3％ (56/60) in the posterior horn; the incidence of medial meniscus tear in the root of the posterior horn was 23.3％ (14/60); the incidences of cartilage degeneration in medial tibial plateau and medial femoral condyle both were 100％ (60/60); a significant negative correlation was observed between dimension of tibiofemoral angle and extrusion distance of medial meniscus.In control group,the extrusion distances of medial menisci ranged from 0 to 2.61 mm (average,0.57±0.80 mm); four patients had genu varum,and the tibiofemoral angle in all patients was 180°; the incidence of medial meniscus injury was 0 in the anterior horn,16.7％ (10/60) in the body,and 70.0％ (42/60) in the posterior horn; no medial meniscus tear was found in the root of the posterior horn; the incidence of cartilage degeneration was 26.7％(16/60) in medial tibial plateau and 30.0％ (18/60) in medial femoral condyle.The odd ratio of meniscus injury and the number of genu varum (extrusion group/control group) was 6.0 and 15.0,respectively.Compared with the control group,the incidences and severities of medial meniscus injury and cartilage lesion of medial tibiofemoral joint were higher in extrusion group.Conclusion Genu varum may be one cause of medial meniscal extrusion.Medial meniscal extrusion increases incidence of medial meniscus injury and has a significant influence on generation and development of osteoarthritis in medial tibiofemoral joint.