Post-transcriptional regulation of prokaryotes is of pivotal importance to the quality control and stability main -tenance of multiple proteins .The ubiquitin and ubiquitin-like proteins-mediated modifications have widely participated in the manipulation of cell cycle control , subcellular localization and cellular metabolisms .Typical ubiquitin like proteins (UBLs) are ubiquitin,SUMO,Nedd8 and FAT10.Recently, the dynamic regulation mechanisms between sumoylation and de-sumoylation have become the cutting edge in the era of ubiquitin-like modifications.In this review, we briefly summa-rized the entire SUMO-mediated modification systems and the intricate balance regulation .We also discussed the counter acting star molecule SENP1 and its relationship with tumor initiation and progression ,immunological regulation and develop-mental homeostasis .

Regulatory T (Treg) cells exert significant influence on the control of autoimmunity and maintenance of homeostasis,while the progressive roles of Treg cells in solid tumor and hematological malignance have also attracted much attention recently.Except for the specific expression of transcription factor forkhead box protein 3 (Foxp3),natural Treg (nTreg) cells have Treg-specific hypomethylation pattern in a limited number of key genes,which is indispensable for stable Treg cells and functional nTreg cells to define the T-cell subpopulation possessing Treg-type epigenome more accurately.The expression of Foxp3 and Treg-specific hypomethylation phenotype have close relationship with the epigenetic regulation,which involves DNA methylation,histone methylation,acetylation,microRNA,etc.This review summarizes the different mechanisms of epigenetic regulation according to the two important features of Treg cells.

OBJECTIVE:To explore the material features and biocompatibility of coronary artery stent and related complications following implantation.METHODS:The databases of CNKI and Medline were searched by the first author using key words of "coronary artery stent,material,biocompatibility and complications" both in English and Chinese.Meta analysis or repetitive studies were excluded,and 16 papers were included in this analysis.RESULTS:Stent materials play an important role in reducing inflammatory reaction and restenosis following stent implantation.As a medical material for humans,the features of stent should be meet the requirements of reliable distensibility,mechanical persistence,good flexibilit and geometrical stability,possible small diameter,excellent blood compatibility,as well as good corrosion resistance.All of these depended on the mechanical and physical features of materials.CONCLUSION:The study of drug-coating stent and drug-eluting stent reduced the restenosis rates following implantation,and the biodegradable stent also attracted more attention.When treating coronary heart disease with cardiovascular stent,the complications should be avoided except consideration of the successful rate of reperfusion.

Cellular autophagy is a major degradative pathway for clearance of aggregate-prone proteins and damaged organelles. It plays an important role in regulating cellular homeostasis, cell growth and development, and disease development. Dysfunctional autophagy contributes to the pathology of various neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease and Huntington's disease, in which specific pathological protein accumulation occurs. A growing body of evidence suggests that resveratrol plays a significantly role in the regulation of autophagy and clearance of pathological proteins. Resveratrol is a potential drug for neurodegenerative diseases therapy. This review focuses on the effects of resveratrol on cellular autophagy and clinical application in the control of neurodegenerative diseases.
Alzheimer Disease ; Autophagy ; Humans ; Huntington Disease ; Neurodegenerative Diseases ; drug therapy ; Parkinson Disease ; Stilbenes ; pharmacology

To provide an effective approach to select, develop, interpret and apply well-designed quality indicators. Then to promote the continual improvement of laboratory quality and to assure patient safety. We focused on the quality cycle of selecting, development, implementation and processing for quality indicators. And some examples for essential activities in the development and use of quality indicators were shown. Through the presentation of process and examples, we put forward an organized approach that provides the laboratory with a plan to structure its development of quality indicators to gather useful information. Quality indicators are important. However, they should be integrated with internal review plan, External Quality Assessment (EQA) and the process of activities in plan. Only then can we really promote the continual improvement of quality.

?AIM:To explore the efficacy and safety of operation in treating secondary glaucoma caused by contusion hyphema.?METHODS: Seventy patients (70 eyes) with secondary glaucoma caused by contusion of anterior chamber in our hospital from November 2014 to November 2015 were selected.All patients were treated by operation.Visual acuity, anterior chamber depth, mean intraocular pressure and postoperative complications of patients before and after operation were compared.?RESULTS:Patients with secondary glaucoma caused by contusion of anterior chamber had better visual acuity after operation and the difference was statistically significant (t=42.471, P<0.001).And they had deeper anterior chamber depth and lower mean intraocular pressure, and the differences were statistically significant (t=9.726, 26.041; P<0.001).Two of those patients had emergence of hyphema after operation and symptomatic relieved after treatment. None of the patients had complications such as blood-staining of cornea or eye pain.? CONCLUSION: The curative effect of operation in treating secondary glaucoma caused by contusion of anterior chamber is good and safe and it can improve visual acuity, deepen the anterior chamber angle depth and reduce the intraocular pressure.

Quantitative immunoassay technique is the common method of quantitative detection in clinical laboratory. Several important branches of quantitative immunoassay were formed by changing the tracer or the Antigen-antibody complex separation method, including radioimmunoassay, fluorescent immunoassay, enzyme immunoassay, chemiluminescence, colloidal gold, immuno-turbidimetric analysis and homogeneous immunoassay. Different immunoassay techniques have their own characteristics, also apply to different detecting conditions in clinic. This paper reviewed several common kinds of quantitative immunoassay technology, and discussed both their advantages and disadvantages, which provide reference for the application and development of clinical testing technology.

The theory of Medical Immunology is independent,which includes a lot of new knowledge.Optimizing resource of medical immunology,recombinating knowledge structure,building knowledge framework and leading in PBL(Problem Based Learning) teaching method help to arouse students’studying interest and elevate teaching effect.

OBJECTIVE:To investigate the influence of dexmedetomidine combined with fentanyl on analgesic effects and hemodynamics of orthopedics patients after surgery.METHODS:A total of 80 patients undergoing orthopedics surgery were randomly divided into observation group (40 cases) and control group (40 cases).Observation group was given patient-controlled analgesia (PCA) of Dexmedetomidine hydrochloride injection+Fentanyl citrate injection,maintaining analgesia for 48 h.Control group was given PCA of Fentanyl citrate injection,maintaining analgesia for 48 h.The MAP,SpO2,HR,VAS and the occurrence of ADR were observed in 2 groups from the application of multi-function monitor to connecting to PCA (T0),12 h after connecting (T1) and 48 h after connecting (T2).RESULTS:At T0,there was no statistical significance in VAS score between 2 groups (P＞0.05).VAS scores of 2 groups at T1-2 were significantly higher than at T0.Those of 2 groups at T1 were significantly higher than at T2;but the observation group was significantly lower than the control group,with statistical significance (P＜0.05).There was no statistical significance in MAP,SpO2 or HR between 2 groups at different time points (P＞0.05).The incidence of respiratory depression,sharp pain,nausea and vomiting,hypotension in observation group were significantly lower than control group,with statistical significance (P＜0.05).CONCLUSIONS:Dexmedetomidine combined with fentanyl show significant analgesic effect after orthopedics surgery,and have no effect on hemodynamics with good safety.

Neurotrophin 3(NT-3) is required for proprioceptive neuron survival and physiological function.During embryonic development,NT-3 deficient in developing muscle dues to loss of proprioceptive neurons in dorsal root ganglia or their axons by apoptosis,and then to the absence of muscle spindles.The chemoattractant effect of NT-3 on dorsal root ganglion axons has been improved that NT-3 is a key requirement for the development of proprioceptive projection to motor neurons.Depletion of NT-3 in the period of adult educes to the decline of the function of proprioception.NT-3 has extensive protective effect on proprioceptive neuron and enhances them to restore the normal physiological function under the pathological state.