Objective To investigate the clinical effect of the MBT straight wire technique for treatment of angle class Ⅱ 1 patho-occlusion.Methods 62 cases of adult class Ⅱ division 1 patients were randomly divided into the observation group (32 cases) and the control group (30 cases).The observation group was treated with MBT straight wire appliance therapy,the control group received the traditional straight wire appliance therapy.The two groups were given cephalometric film before and after treatment,and the clinical effect was observed and recorded.Results In the observation group,there were no significant differences in SNA,SNB,ANB,LI-MP,MP-SN after treatment compared with before treatment (t =0.46,0.73,0.24,1.05,P ＞ 0.05),while there were statistically significant differences in ANB,U1-SN,U1-L1 after treatment compared with before treatment (t =1.58,6.32,7.43,P ＜ 0.05).Before treatment 7 mm of openbite,OB 2 mm after treatment,returned to normal overbite and overjet,there was statistically significant difference(t =3.26,P ＜ 0.05).The mean treatment time was (22.0 ± 2.5) months in the observation group,it was significantly lower than(3 1.0 ± 3.8)months in the control group,there was statistically significant difference (t =7.47,P ＜ 0.05).After treatment,U1-SN,U1-L1 and OB in the observation group were significantly better than those of the control group,there were statistically significant differences(t =5.47,3.43,5.41,1.67,all P ＜ 0.05).Conclusion MBT straight wire appliance has the advantages of simple operation,accurate positioning of teeth,simplified correction procedure and short treatment time,it is worth the clinical promotion.

Objective To investigate the cholesterol-reducing effects of complex bacteria leaven (CBL) from Kefir Grain milk yoghurt in experimental hypercholesterolemia rats. Method Isolated three strains of Lactobacilli from Kefir Grain milk yoghurt with high activity of bile salf hydrolase and cholesterol-reducing effect and prepare the CBL. Forty rats were randomly divided into four groups: control group, hypercholesterolemia model group, and experimentalⅠ, Ⅱ groups according to serum TC, TG, HDL-C and LDL-C concentrations. Control group was fed basic feed and three other groups were fed high fat feed. Experimental groups were given i.g. the test substances for 8 w, and control and model groups were given water, then serum TC, TG , HDL-C and LDL-C concentrations were determined at the end of the 2nd, 4th, 6th and 8th w. Results Compared with model group, serum TC, TG and LDL-C in experimentalⅠgroup and Ⅱgroup were significantly decreased and HDL-C increased after 8 w of treatments respectively, groupⅠ better than groupⅡ. Conclusion Complex bacteria leaven from Kefir Grain show significant effects of cholesterol-reducing effect in experimental hypercholesterolemia rats.

Objective To investigate the cholesterol-reducing effects and tolerance property of Lactobacteria isolated from Kefir grain in vitro. Method In imitating gastroenteric environments,such as low pH (2.0～4.0) and high bile salt concentration (0.2%-0.4%),the survival counts of Lactobacteria was carried out and cholesterol-reducing effects were studied. Results The strains of KTX,KS4 and KL1 had more survival counts in eaperimental condition and KTX. KS4 were isolated as more tolerant strains to gastro-enteric environments and could reduce cholesterol in vitro. Conclusion Lactobacteria isolated from Kefir grain had tolerant properties and cholesterol-reducing effects in vitro.

OBJECTIVE To study the therapeutic effect of ceftazidime(CAZ) in a rat model of pneumonia caused by CTX-M-14 extended-spectrum ?-lactamases(ESBLs)-producing Klebsiella pneumoniae which is susceptible to ceftazidime in vitro.METHODS Forty eight rats were divided into four groups randomly which were ceftazidime-treated group,piperacillin/tazobactam-treated group,ceftaxime-treated group and the control group.All rats were intranasally inoculated with bacterial suspension of CTX-M-14 ESBLs-producing K.pneumoniae to produce pneumonia models.Twenty four hours after inoculation,rats were intramuscularly administered ceftazidime,piperacillin/tazobactam,ceftaxime,or saline,respectively.The temperature,leucocyte count,percentage of neutrophils,and bacterial counts in the right lungs of rats were detected at some timepoints.RESULTS Compared with the ceftaxime-treated group and the control group,the leucocyte count,percentage of neutrophil count and common logarithm of the bacterial count in the right lungs of the ceftazidime-treated group and piperacillin/tazobactam-treated group after the treatment which lasted seventy two hours were significantly lower than those of ceftaxime-treated group and the control group(P0.05).CONCLUSIONS Ceftazidime shows good therapeutic effect in pneumonia in rats caused by CTX-M-14 ESBLs-producing K.pneumoniae which is susceptible in vitro.

Aminoacyl-tRNA synthetase catalyzing the first reaction of protein biosynthesis. In mammalian cells, eight aminoacyl-tRNA synthetases (aaRSs) and three auxiliary protein factors form a macromolecular aminoacyl-tRNA synthetases complex (aaRS complex). The three nonsynthetase protein factors, namely, p43, p38, and p18 were found to be involved in many other important life activities besides their roles in the complex. The auxiliary factor p43 was the precursor of endothelial monocyte activating polypeptideⅡ (EMAPⅡ), which involved in angiogenesis and apoptosis. The auxiliary factor p38 was crucial for the development of lung, and its abnormal accumulation in neuron would be related to the Parkinson’s disease. The auxiliary factor p38 and p18 could promote the repair of DNA damage via different pathways in a highly organized way. All these breakthroughs enhance our understanding about the interaction between the aaRS complex and the macromolecular signaling network and promote the studies on this field.

Objective To know about the reason of nursing students suffering workplace bullying. Method 25 interviewees were recruited by snowball sampling. The data were collected through the analysis of focus group interview. Results The interview data were analyzed by Colaizzi analysis method and three themes were extracted.They are the nursing students′own factors; organizational management factors;social and cultural factors. Conclusions According to the reasons for the nursing students suffered workplace bullying, formulating corresponding measures has an important role in improve the quality of clinical practice and the stability of nursing team.

OBJECTIVE To investigate the infection condition and associated causative factors in our hospital in order to improve the methods of monitoring,preventing and controlling infection.METHODS All patients admitted in 24 hours were investigated.including record review and patient examination.Participants mainly consisted of the specialized staff,with assistant administrative staff for hospital infection.The investigating table was filled for record review and patient examination respectively.Statistical analysis was performed.RESULTS 119 of the 123 hospitalized patients were investigated(96.8%).The current rate for hospital infection was 6.7%.The most infected site was respiratory tract.Antibiotics was used in 39.5% of the patients.CONCLUSIONS The current method can be used for investigating the present state of hospital infection.Based on the investigation,objective monitoring,intensified specimens detection,and reasonable antibiotics administration can be performed to decrease the incidence of hospital infection.

Objective To develop a Chinese version questionnaire of psychosocial adaptation for breast cancer patients with good reliability and validity.Methods The preliminary questionnaire was formed by extensive literature review,semi-structured interview for 10 breast cancer patients and consultation for 4 experts.In order to analyze items and test reliability and validity,228 postoperative breast cancer patients were selected.Results Psychosocial adaptation questionnaire included 44 items and 5 dimensions.It had good content validity and construct validity.With the method of principle components analysis,and five factors were extracted,the cumulative contribution rate was 54.578％,loading for most factors was over 0.4.The result indicated that the structure of the questionnaire was similar to the theory construction.The criterion-related validity with self-rating anxiety scale,the general self-efficacy scale,and self-acceptance questionnaire was-0.757、0.505、0.691.Cronbach's α coefficient of the overall questionnaire was 0.945,retest correlation coefficient was 0.961.Conclusions The developed questionnaire showed good reliability and validity.It can objectively and effectively evaluate psychosocial adaptation of breast cancer patients,so it has practicality and popularization value.

Objective To investigate the expression of heme oxygenase-1 (HO-1) in type 2 diabetes mellitus (T2DM) as well as hidden diabetes mellitus (HDM) and explore its clinical significance.Methods A total of 384 suspected patients with T2DM having underwent oral glucose tolerance test were enrolled in this study:216 cases of patients were T2DM (T2DM group),and 168 cases of patients were HDM (HDM group).T2DM patients were divided into single-vessel lesion group(122 cases),double-vessel lesion group(54 cases),and more-vessel lesion group(40 cases).Another 60 healthy person were as control group.The intima-media thickness (IMT) of carotid was measured by ultrasonography.The level of serum HO-1 was detected with enzyme-linked immunosorbent assay.Results The serum level of HO-1 in control group,HDM group and T2DM group was (1.24 ± 0.53),(2.12 ± 0.84),(3.46 ± 1.23) μ g/L.The serum level of HO-1 in T2DM group was significantly higher than that in control group and HDM group (P ＜ 0.05),and in HDM group it was significantly higher than that in control group(P ＜ 0.05).There were significant differences in the serum level of HO-1 and IMT of carotid among single-vessel lesion group,double-vessel lesion group,and more-vessel lesion group [(2.94 ± 1.14),(3.72 ± 1.36),(4.64 ± 1.58) μg/L and (1.21 ±0.16),(1.44 ± 0.20),(1.62 ± 0.27) mm] (P ＜ 0.05).The serum level of HO-1 and IMT of carotid in double-vessel lesion group was higher than that in single-vessel lesion group,and in more-vessel lesion group it was higher than that in double-vessel lesion group and single-vessel lesion group (P＜ 0.05 or ＜ 0.01).The serum level of HO-1 was positively related with IMT of carotid (r =0.512,P ＜0.01).Conclusion The high-level expression of HO-1 is found in HDM and T2DM patients,which maybe play a key role in the early diagnosis of T2DM.

Objective To retrospec the efficacy of percutaneous nephrolithotomy on treatment of urinary tract stones in solitary kidney.Methods From June 2008 to April 2011,percutaneous nephrolithotomy technique was used to treat 20 cases of urinary tract stones,with kidney stone or cast form staghorn calculi in 4 cases,single pelvis,calyceal stones 10 cases,6 cases of upper ureteral calculi;Fourteen cases used the standard channel F24;6 cases used F16 Micro Channel.Among the 20 patients,16 cases performed stage Ⅰ single channel surgery;2 cases performed dual-channel surgery in Ⅰ stage;2 patients with severe infection performed stage Ⅱ single channel surgery.Results The average duration of the operation was 66mins.The stone clearance rate was 88.36％ on average(18/20),without laceration or bleeding of pelvis or renal calyces mucosa or any other complications.Postoperative indwelling nephrostomy tubes were removed 6 -7 days later,without complications found during the follow-up period.For the 6 cases with preoperative renal dysfunction before surgery,the levels of blood Cr decreased at 2 weeks after surgery.Conclusion Compared to open surgery,percutaneous nephrolithotomy is safe and effective with small wound in treatment of solitary kidney with invasive upper urinary tract calculi.