Objective To detect multidrug resistance gene(mdr1) transcription in peripheral blood lymphocyte(PBL) before and after chemotherapy and to determine its correlation with chemotherapeutic effect.Methods The peripheral blood sample was taken from each lung cancer patients(n=32) before chemotherapy and at 3rd week after chemotherapy and subjected to fluorescent quantitative real-time RT-PCR.Simultaneously the tumor size was determined by chest X-ray or CT.Results The mdr1 mRNA level in PBL after chemotherapy increased significantly as compared with that before chemotherapy [(4.316 6?0.923 1) vs(3.881 8?0.828 0),t=-4.207,P=0.000].There was an negative correlation between mdr1 mRNA level and the tumor size(r=-0.389,P=0.028).The level of mdr1 transcription in SCLC patients was significantly less than that of NSCLC patients before chemotherapy [(2.831 4?0.398 4) vs(4.231 9?0.603 4),t=6.101,P=0.000].Conclusion In terms of an anticancer drug resistant mechanism,there could be a difference between NSCLC and SCLC.Longitudinal study of mdr1 level in PBL is valuable for evaluating the treatment(response.)

OBJECTIVE: To implement the policy of New Medical Reform, and to promote pharmaceutical care of our hospital. METHODS: The policy of New Medical Reform was carried out and specific implementation methods for the improvement of pharmaceutical care of our hospital were analyzed. RESULTS & CONCLUSION: Prescription evaluation was applied to provide pharmaceutical care in the clinic indirectly. Clinical pharmacists went into the wards to provide direct pharmaceutical care. They supervised the utilization of antimicrobial drugs, carried out pharmacy propaganda and wrote Clinical Drug News. ADR monitoring was also carried out. The policy of New Medical Reform was implemented through above methods to promote pharmaceutical care of our hospital.

Objective:In order to develop a new standard of curative effect,this study was designed to track the number of CTCs in patients with lung cancer during chemotherapy.Methods:Samples of peripheral blood was taken from each lung cancer patients(n=32) on the day before chemotherapy as well as the third week after the chemotherapy cycle.The samples were subjected to real-time fqRT-PCR.Meanwhile the tumour size was determined by chest X-ray or computed tomograghy. Results:Compared to that of pre-chemotherapy,the expression level of CK19 in the patients significantly declined after chemotherapy(t=4.659,P=0.000 1).The level of CK19 mRNA in patients with SCLC was higher than that of patients with NSCLC(t=1.944,P=0.041).The change of CK19 mRNA correlated well with the type during the treatment.Relatively the shift of SCLC is more obvious(t=6.073,P=0.000 1).Conclusions:The real-time fluorescent quantitative-PCR approach is useful for measuring the relative number of CTCs in a patients' peripheral blood to monitor the effectiveness of treatment,and for designing more comprehensive and reasonable therapeutic regimes at earlier dates for patients.

Objective: To investigate the clinical value of immature reticulocyte count in early monitoring the suppression of bone marrow function in patients with chest tumor during postoperative chemotherapy.Methods:Forty-one patients with chest tumor(22 with lung cancer?19 with esophagus cancer) received chemotherapy after radical operation.Several indexes including reticulocyte count and white blood cell(WBC) were detected with sysmex SE-9000 automated analyzer during the procedure of postoperative chemotherapy.Results:Compared with the value before chemotherapy,HFR+MFR was significantly lower than that at 7 days after chemotherapy(P

Objective:To evaluate the real-time fluorescent quantitative-polymerase chain reaction approach(fqRT-PCR) of cytokeratin 19(CK19)targets for its feasibility to estimate the number of circulating tumor cells(CTCs)in the patients with lung carcinoma during radiotherapy and to correlate our observation to clinical findings.Methods:Samples of 5ml peripheral blood were taken from each lung cancer patients(n=30)both before and after the radiotherapy course.Meanwhile tumour size was determined by chest X-ray or computed tomography.The blood samples were subjected to real time RT-PCR assay.All patients with lung cancer were treated with primary definitive and mediastinal radiotherapy.Results:Compared to with that before the-treatment,the value of CK19 mRNA in PB after therapy decreased dramaticall(y5.093 2?1.062 8 vs 4.249 3 ?0.832 3,t=3.192,P=0.007).The change of CK19 mRNA level before and after radiotherapy was closely related to the typ(esquamous cell cancer/adenocarcinoma vs.small cell cancer,0.538 9?0.903 0 vs 1.682 6?0.946 7,t=2.146 5,P=0.051).Meanwhile,there was a close link between the grade(Well/Mod vs Poor)and the change of CK19 mRNA(0.502 4 vs 1.527 1,t=2.017,P=0.065).The change of CK19 mRNA level in peripheral blood(PB)was related to variation of tumour burden during radiotherapy(r=0.057 5,P=0.025).Of the 30 cases studied,24 cases were positive before radiotherapy (24/30,80%).The positive rate was 53%(16/30)after radiotherapy,meaning that 8 cases were converted intonegative after radiotherapy. Conclusion:The disseminated cancer cells cannot be completely eradicated by radiotherapy,suggesting further more systemic adjuvant treatment should be conducted.Early assessment of the therapeutic response might be possible by serial quantitative of CTCs.Detection of tumour cells disseminated in peripheral blood can provide clinically important data that are of value for tumour staging and possibly for prognostication.

OBJECTIVE:To investigate the characteristics and regular pattern of the adverse drug reactions (ADR) occurred in our hospital.METHODS:A total of 174 ADR cases reported by our hospital from May 2006 to June 2008 were categorized and analyzed statistically.RESULTS:The ADR were induced by 16 categories(83 kinds) of drugs,predominantly by the antimicrobial agents(12 kinds,60 cases) and Chinese drugs preparations(17 kinds,66 cases).The ADR of 57 cases(26.89%) manifested chiefly as lesions of skin and its appendages,followed by gastrointestinal lesion(39 cases,18.40%).CONCLUSION:Timely ADR reporting is conducive to ADR incidence reduction and clinical rational drug use.

ObjectiveTo investigate the effect of psychological intervention on quality of life (QOL) of patients with mammary carcinoma.Methods64 patients with mammary carcinoma were divided into intervention group (n=36) and control group (n=28), and psychological status and QOL of patients of two groups were analyzed after treatment.ResultsThe psychological status and QOL of intervention group was better than that of control group (P<0.05～0.001).ConclusionThe psychological intervention in full stage being in hospital can improve QOL of patients with mammary carcinoma, and it is clinically significant to improve cure rate and survival rate of patients.

Objective To study the arterial stiffness in maintenance hemodialysis (MHD)patients and to explore the associated factors. Methods Ninety MHD patients and 36 healthy controls were enrolled in the study.Automatic pulse wave velocity (PWV) measuring system was applied to examine carotid-femoral pulse wave velocity(CFPWV)and carotid-radial PWV(CRPWV)as the parameters reflecting central elastic large arterial and peripheral muscular medium-sized arterial elasticity respectively.Blood pressure and biochemical parameters were detected.Backward muhiple linear regression analysis was used to assess the influencing factors of arterial stiffness.Results CFPWV and CRPWV in MHD patients were greater than those of healthy controls [(13.22±3.23)m/s vs(10.67±2.11)m/s and(9.58±1.87)m/s vs(8.81±1.09)m/s,all P＜0.05].Backward multiple regression analysis demonstrated that age and pulse pressure were positively correlated with CFPWV,while dialysis duration,calcium phosphorus product,parathyroid hormone,low density lipoprotein and mean arterial blood pressure were signiticantly correlated with CRPWV.Volume reduction after single dialysis session could not improve arterial stiffness ofMIlD patients.Conclusions Arterial stiffness of large artery and peripheral muscular medium-sized artery increases in MHD patients.Age and pulse pressure are major determinants of large artery stiffness.Peripheral muscular medium-sized arterial stillness is more easily influenced by dialysis-associated factors.

Objective:To investigate if CD4~+CD25~+ regrating T cells can suppress GVHD mediated by CD4~+ T cells in murine semi-allogeneic bone marrow transplantation model and try to figure out the mechanisms evolved.Methods:C3H(H-2~k) as donors, C3H?B6(H-2~ k/b) F1(recipients) were lethally irradiated and reconstituted with antibody T cell depleted bone marrow(ATBM), and divided into 4 groups: (A)No further treatment(n=10);(B)Infused 5?10~5 C3H CD4~+T cells(CD4,n=10);(C)Infused 5?10~5 CD4~+CD25~+Treg(CD25,n=5);(D)Infused 5?10~5 CD4~+T cells plus 5?10~5 CD4~+CD25~+Treg(CD4/CD25,n=6).Results:All mice in ATBM group, CD25 group and CD4/CD25 groups survived until 60 days without any GVHD symptoms, while all mice in CD4 group died in 10 days from GVHD.Conclusion:CD4~+CD25~+Treg cells did not cause GVHD and can prevent GVHD mediated by CD4~+ T cells while co-injected with CD4~+ T cells.

Objective To explore the examination methods about stability of carotid atherosclerotic plaque and evaluate the curative effect of medicine on carotid atherosclerosis.Methods 150 patients with carotid atherosclerotic plaque in acute stage ischemic stroke were divided into treatment group and control group at random.Stable or instable plaques were assessed and carotid artery intima-media thickness(IMT) were measured according to the characteristics of carotid atherosclerotic plaque on supersound imagin.The treatment in treatment group were different from that in control group.The therapeutic efficacy was observed respectively.Results After the treatment 1 month and 3 months,in treatment group carotid artery IMT decreaced more than that at accidence of treatment group and that of control group,the carotid atherosclerotic plaques stabilizese increasingly.Conclusion The stability of carotid atherosclerotic plaque may be evaluated through the ultrasound imagin.Statin and aspirin can lighten carotid atherosclerosis and stabilize the plaque.