Objective to investigate the effects of liraglutide on omentin-1 and insulin resistance in high-fat diet rats. Methods Forty male Wi-star rats were randomly divided into four groups,and respectively fed with normal diet(NC group),high-fat diet(HF group),high-fat diet with small doses of liraglutide injected intraperitoneally(HF+Lira 1 group),and high-fat diet with large doses of liraglutide injected intraperitoneally (HF+Lira 2 group). After 16 weeks,the body weight,serum lipid levels,omentin-1 and insulin were measured,and then the insulin resistance in-dex were calculated. Results the body weight,the levels of tG,and the insulin resistance index in HF group were higher than NC group,omentin-1 was lower,and the differences were statistically significant(P < 0.05). tC,LDL-C were elevated,HDL-C was reduced,but there were no statisti-cally differences(P > 0.05). Compared with HF group,the body weight,the levels of tG,and the insulin resistance index in HF+Lira 1 group and HF+Lira 2 group were lower,omentin-1 was higher,the differences were statistically significant(P < 0.05). Furthermore,the effects of HF+Lira 2 group were more pronounced. Conclusion Liraglutide can restrain high-fat diet induced obesity,and improve insulin resistance through elevated the level of omentin-1 in dose-dependent manner.

Objective To investigate the combination effect of cetuximab and irradiation on colorectal carcinoma CL187 cell line and underlying molecular mechanism.Methods CL187 cells with or without cetuximab treatment were irradiated by 0,4 and 8 Gy X-rays,then cell death percentage was determined by MTT 24 and 48 h post-irradiation.Clone forming assay was used to evaluate the cell reproliferation ability.Cell cycle distribution,apoptosis,and necrosis were analyzed by flow cytometry.Western blot was used to detect the protein expressions of DNA-PKcs,Ku70 and Ku80.Results The cetuximab enhanced the percentage of radiation-induced cell death,while descreased the cloning formation capacity and increased radiosenvtivity (t =-6.14、-6.53,P ＜0.05).The SER of cetuximab on CL187 cell line approached to 1.38.In addition,cetuximab also increased radiation-induced G0/G1 phase arrest (t=-4.64,P＜0.05) and the percentage of apoptosis and necrosis (t=-9.16,P ＜0.05),but it descreased the expression levels of DNA-PKcs,Ku70 and Ku80 proteins.Conclusions The cetuximab treatment might enhance the inhibitory effect of irradiation on colorectal carcinoma CL187 cell line by influencing cell cycle distribution,cell apoptosis,and the expression of DNA repair proteins.

OBJECTIVE To establish multiple PCR(M-PCR) assay for simultaneous detection of Treponema pallidum(TP) and herpes simplex virus(HSV),Haemophilus ducreyi(HD).METHODS According to specific gene sequence of the three agents:HSV DNA polymerse gene,TP tpp47 gene,HD 6s rRNA gene;three sets of specific primers were designed and a multiple PCR assay was developed to detect 3 agents in one test.RESULTS The target DNA of 3 agents were specifically amplified by their specific primers,the appropriate size of four DNA fragments was 202bp,252bp,152bp for TP,HSV and HD,respectively.The DNA of other several common microbes of genital tract and human genome could not be amplified by three sets of primers.CONCLUSIONS Primary study indicated that the M-PCR assay can simultaneously,specifically and rapidly detect out HSV,TP and HD.

Objective To understand the incidence of common geriatric syndromes in hospitalized patients,and analyze the relationship between different kinds of geriatric syndromes.Methods A crosssectional study was conducted by five well-trained investigators-300 patients were chosen from geriatric department and medical department within 3 top three hospitals in Chengdu.Results 300 copies of questionnaires were distributed in total,and 300 questionnaires were returned (response rate 100％).Among those,277 questionnaires were considered valid (valid response rate 92.3％).Among those elderly which were above 65 years old,having risk of falling accounted for 91.3％,feeling pain accounted for 79.1％,chronic constipation accounted for 59.2％,having risk of malnutrition accounted for 49.1％,malnutrition accounted for 28.2％,having risk of pressure ulcers accounted for 32.5％,incontinence accounted for 25.3％,and dysphagia accounted for 17％.The correlation among these syndromes was signifcant.Conclusions The overall incidence of geriatric syndromes in hospitalized patients is higher than that in the community.These syndromes are correlated with each other.One can be a risk factor of other geriatric syndromes and also could affect each other.

OBJECTIVE:To study the analgesic and anti-inflammatory actions of essential oil extracted from Radix Angelica Sinensis METHODS:The pharmacodynamic effects were obsersed in five experimental models of inflammation and pain,including:(1)mouse auricular edema induced by xylen;(2)increased vascular permeability induced by acetic acid in mice;(3)paw edema caused by albumen in rats;(4)granuloma caused by implanted cotton ball in rats;(5) writhe reaction induced by acetic acid in mice RESULTS:Essential oil from Angelica Sinensis could distinctively inhibit the inflammatory edema caused by various inflammatory agents and reduce the times of writhe induced by acetic acid in mice CONCLUSION:Essential oil from Angelica Sinensis has analgesic effect and could not only inhibit acute inflammation but also chronic ones in animals

Purpose To investigate the imaging features of lumbar spinal epidural angiolipoma, and to improve the imaging diagnostic capability of the disease. Materials and Methods Four patients with lumbar spinal epidural angiolipoma confirmed by pathology were recruited in the study. CT and MRI images were reviewed and the imaging characteristics including the shape, size, location, density, and signal intensity were analyzed. Results The lesions located at the right front of the spinal canal in two cases, at the left front in one case, and the residual one case located directly behind the spinal canal. The longitudinal axis of the lesions paralleled to the longitudinal axis of the spine. The dura mater spinalis was compressed and inward shifted. All of the four cases showed homogeneous iso- or hypo-density on CT without calcification or necrosis in the lesions. The maximum diameter was 3 to 5 cm. The boundary was clear and smooth. Three lesions showed dumbbell-shaped and crossed foramen, but the adjacent bone were not absorbed or destructed. One lesion showed scallop-like. On MRI, four cases displayed slightly hypointense on T1WI and hyperintense on T2WI. On STIR images, the lesions showed hyperintense with patchy low signal intensity in it. The boundary of the lesions was clear. After administration of contrast media, two lesions presented remarkably homogeneous enhancement, one lesion showed dual tail sign, and one lesion displayed pen-tip-like at the both ends. Conclusion MRI plays an important role in locating the lesion and distinguishing internal tissue components of spinal angiolipoma, which is the gold standard for the diagnosis of the tumor. CT provides excellent supplement. The Combination of CT and MRI will improve the diagnostic accuracy of the spinal angiolipoma.

Objective To compare the doses of shrsine with the basal insulin of continuous subcutaneous insulin infusion(CSII) and the fluctuation of blood glucose (BG)in patients with diabetes mellitus from CSII to glargine-based multiple dally insulin injections therapy.Methods One hundred and two type 2 diabetic patients achieved ideal glycemia control with CSII,then transferred to slarsine-based multiple daily insulin injections therapy.The doses of glargine with the basal insulin of insulin pump and the flucmarion of BG were compared in type 2 diabetic patients from CSII to shrgine-based multiple daily insulin injecfions therapy.Results When the fasting BG achieved an ideal level,the basal insulin doses of CSII were(0.30±0.11)U/(kg·d),and the doses of ghrgine insulin were(0.28±0.09)U/(kg·d).However,the difference between the two methods was no statistically significant.The mean level of BG of CSII and multipie daily insulin injections was(7.94±1.32)mmol/L and(7.49±1.34)mmol/L respectively,the mean BG levels of two methods were more than their three times sample standard deviation.Conclusion Glargine insulin Callphya similax role in the basal insulin of CSII.

Objective To investigate the status quo of job-family and family-job conflicts among the nursing of the operating room nurses.Method A total of 190 nurses from an operating room participated in the survey by demographic questionnaire and work-family conflict scale.Results The average score on job-family was (3.15 ± 0.48) and there were significant differences between family-job conflict and job-family (2.75 ± 0.27 vs.3.55 ± 0.61) (t =-10.349,P＜0.05).There were significant differences in job-family conflict scores (P ＜0.05) in view of different age,working years,professional titles,number of night shifts per month,marital status and daily working hours.There were significant differences in family-job conflict subscale scores (P＜0.05).Conclusions The job-family conflicts of the nurses in the operating room is at the middle level.The perceived job-family conflict is higher than that of the family-job conflict.Such factors as age,length of work,professional tide,number of night shifts per month,marital status and daily working hours can lead to job-family conflict among the operating room nurses.The nursing administrative should displace nursing personnel and optimize the shifts so as to reduce the rate of job-family conflicts.

OBJECTIVE To discuss the available methods of preventing the mattress pollution in the wards of the(hospital), and to keep the mattresses clean and dry and eliminate the hidden danger of nosocomial infection.(METHODS) The mattress protective covering is used of a new type of textile materials,and then applied into clinic.Selected 100 pieces of mattresses in the wards,divided them equally into two groups at random,the(experimental) group and the control group.After cleaning and pasteurization(surface) sampling and bacterial culturing for every mattress were undertaken.For the experimental group,spread the protective(covering) before the sheet,and for the control group,used the sheet directly.The colony number of each group was compared in the 3rd,7th,and 14th days.RESULTS The mattresses of experimental group were clean,light polluted and with less colonies,and that of the control group were heavy polluted and with more colonies.The comparisons of the total colony number and the number of every sampling point in the 3rd,7th,and 14th days of the two groups showed that there was a(significant) difference(P

OBJECTIVE To retrospectively investigate the ciprofloxacin-resistance rate of Escherichia coli(ECO) and its main impact factors,and then guide empiric therapy for its infection. METHODS We looked up relative data in Hubei Network for Surveillance of Bacterial Antimicrobial Resistance.WHONET 5.2 Software was used to analyze resistance rates,and SAS software to perform univariate and multivariate Logistic regression assay. RESULTS Ciprofloxacin-resistance rate of ECO was and is on the rising,meanwhile,its resistance rates were different for laboratory,patient origin,age group,gender and so on,and the differences were all very significant(P