Cytochrome P450 monoxygenase converts arachionic acid to four epoxyeicosatrienoic acid regiosomes: 5,6-EET(epoxyeicosatrienoic acid);8,9-EET; 11,12-EET and 14,15-EET.Recent studies show that EETs are involved in signal transduction. EETs open Ca 2+ -sensitive K + channel and inhibit Na + channel,Ca 2+ -sensitive Cl - channel and so on. What is more ,EETs have been demonstrated to activate PP60 c-src and initiate a tyrosine kinase cascade that mediates mitogenic effects.

Epoxyeicosatrienoic acids(EETs)are the metabolic products of arachidonic acid(AA). They have the function of stimulating cell division, inhibiting platelet aggregation and regulating the transfer of cell calcium. In recent years, more and more people paid their attention to the effects of EETs on heart. This article will give a review about EETs and the relationship between EETs and heart.

ObjectiveTo detect serum soluble B7-H4 (sB7-H4) levels in patients with non-small cell lung cancer ( NSCLC ) and explore its clinical significances.MethodsThe sB7-H4 levels the sera of in 102 NSCLC patients,60 benign lung disease patients and 90 healthy controls were determined by ELISA.The relation between serum sB7-H4 levels and NSCLC of pathological staging was analyzed by use of t test.ResultsThe serum sB7-H4 levels in NSCLC,benign lung disease and heathy control groups were (45.61 ±5.67 ),( 30.14 ± 3.51 ) and ( 28.52 ± 4.76 ) μg/L,respectively.The levels of NSCLC group were significantly higher than those of benign lung disease and healthy control groups ( t =2,59 and 3.61,P ＜ 0.05 ).The serum sB7-H4 levels of Ⅲ/IV stage NSCLC patients ( 63.93 t 4.76 ) μgL,with lymph node metastasis (61.73 ±3.97) μg/L and before operation patients (63.29 ±6.19) μg/L were relatively higher thanthose inthe Ⅰ/Ⅱ stage(35.12 ± 3.88 )μg/L, without lymphnode metastasis (40.26 ± 5.39 ) μg/L,after operation ( 40.51± 4.08 ) μg/L.There were significant difference ( t =6.78,5.73 and 3.67,P ＜ 0.05 ).Conclusions Serum sB7-H4 of NSCLC patients increases significantly and correlates with clinical stages and therapeutic conditions.Detecting on sB7-H4 is helpful for lung cancer diagnosis and therapeutic effect evaluation.

A Review The endothelium derived hyperpolarizing factor (EDHF) has been paied attention to since the late 1980s. But it is not clear which are EDHF. The article reviewed the EDHF works and epoxyeicosatrienoic acids (EETs),an important kind of EDHF.

ObjectiveTo explore the levels and clinical significance of osteopontin (OPN) in serum of patients with non-small cell lung cancer (NSCLC).MethodsOPN levels of 102 cases of NSCLC,42 cases of benign lung disease and 30 cases of healthy control were measured by enzyme-linked immunosorbent assay(ELISA).ResultsThe serum OPN levels of NSCLC were significantly higher than those of benign lung disease group and healthy control group (P＜0.01).The levels of OPN in serum were compared between different gender,age,histological differentiation and pathological type groups,but there were no significant difference (P＞0.05).The serum OPN levels of Ⅲ - Ⅳ stage NSCLC patients,with lymph node metastasis,before operation patients,recrudescent patients,survival below 3 years patients were relatively higher than that in the patients with Ⅰ - Ⅱ stage,without lymph node metastasis,after operation,no recrudescent,survival over 3 years patients,and the differences were significant (P＜0.01).ConclusionsOPN levels in serum reflected the severity of the disease,which can be used to determine the effects of surgery and monitor recurrence and evaluate prognosis.

Objective To evaluate the performance of virtual touch tissue quantification imaging (VTQ) for differential diagnosing in uterine hysteromyoma and adenomyoma. Methods Blood flow parameters and SWV (shear wave velocities) from 42 regular hysteromyomas,14 abnormal hysteromyomas and 25 uterine adenomyomas were compared and analyzed through color Doppler. Results The SWV of regular hysteromyomas was on average of (2.72 ± 0.31) m/s;that of abnormal hysteromyomas (1.88 ± 0.25) m/s;while the SWV of uterine adenomyomas was on average of 1.91 ± 0.27 m/s. The mean SWV in regular hysteromyomas group was significantly higher than that in abnormal hysteromyomas group and uterine adenomyomas group (P < 0.05). There was no significant difference of SWV between abnormal hysteromyomas group and uterine adenomyomas group (P > 0.05).The resistance index and SWV of uterine hysteromyomas and adenomyomas had positive correlation (r = 0.753,0.544,0.506,P < 0.05). Conclusion VTQ can quantify the stiffiness of uterine hysteromyoma and adenomyoma.

Objective To compare the optical properties and visual quality between Tecnis ZMB00 and AcrySof ReSTOR SN6AD1 multifocal intraocular lens (MIOL) in patients undergoing cataract surgery.Methods A total of 120 patients (130 eyes) who umderwent elective cataract surgery in our hospital from March 2012 to July 2015 were selected prospectively.All patients were treated with diffractive MIOL,and divided into group A (Tecnis ZMB00 group) and group B (AcrySof ReSTOR SN6AD1 group).The uncorrected distance visual acuity (UCDVA),uncorrected near visual acuity (UCNVA),uncorrected medium distance visual acuity (UCIVA),best corrected distance visual acuity (BCDVA) and best corrected near visual acuity (BCNVA) were measured before and after surgery.The defocusing curve was drawn for analysis of the contrast sensitivity,and the postoperative visual function and survival quality questionnaire (VF-14) was used to evaluate the postoperative visual quality.Results At 1 week,1 month and 3 months after surgery,recovery of UCNVA,UCIVA and BCNVA in group A was better than that in group B (all P ＜ 0.05).At 1 week and 3 months after surgery,the recovery of UCDVA in group A was better than that in group B (all P ＜ 0.05).At 3 months after surgery,the spherical equivalent was lower in group A than group B (P ＜ 0.05).The contrast sensitivities under scotopia,photopic glare and scotopic glare of different frequencies in group A were higher than those in group B (all P＜0.05).The VF-14 score in group A at 3 months after surgery was lower than that in group B (P＜0.05).In group A,0.0 D and-2.5 D formed 2 peaks,and from-2.0 D to-2.5 D formed the platform stage,while in group B,only 0.0 D and-3.0 D formed 2 peaks.Conclusion The implantation of Tecnis ZMB00 MIOL after cataract surgery can improve the uncorrected visual acuity of treated eye,contrast sensitivities,postoperative visual function and quality of life of patients.

OBJECTIVE To investigate spectrum and drug resistance of No-Pseudomonas aeruginosa,to provide the basis for proper use of antibiotics and prevent patients from hospital acquired infection.METHODS API staph analysis system and the K-B agar diffusion test according to NCCLS were used to identify 357 strains of No-Pseudomonas aeruginosa and to performe drug sensitivity test.RESULTS From 2004 to 2008,357 strains of No-Pseudomonas aeruginosa were isolated.There are 233 Stenotrophomonas maltophilia strains(65.27%,233/357),55 Burkholderia cepacia strains(15.41%,55/357).Sputum was the main sourse of No-P.aeruginosa(88.52%,316/357).The isolation rate of No-P.aeruginosa was the highest in intensive care units(38.10%,136/357),the next was department of respiratorymedicine(23.53%,84/357).The results of drug susceptibility test showed that the resistance rate of No-P.aeruginosa to most drugs were higher,only the resistance rates of S.maltophilia to minocycline,gatifloxacin and levofloxacin were lower,(3.26%,20.55% and 20.83% respectively),The resistance rates of B.cepacia to meropenem,piperacillin/tazobactam and Imipenem were lower,being 29.17%,33.33% and 39.62% respectively.CONCLUSIONS The most common strains is S.maltophilia and Burkholderia cepacia,the most common site of infection is lower respiratory tract,Most strains of S.maltophilia are isolated from ICU and department of respiratory diseases.The result of drug sensitive test showed that there were high rates of multiple drug resistance in No-P.aeruginosa and treatment of No-P.aeruginosa infection should be based on the results of the drug susceptibility test.

Objective To identify and quantitate the conjunctival aerobic bacterial flora from healthy people of 3 age groups in Chongqing city.Methods Samples taken with moist swabs from the lower fornix of both eyes of 33 children(9.85?0.57 years old),31 youths(21.23?0.88 years old)and 30 elders(72.97?4.94 years old)were given bacterial cultures.Positive ones were identified and colony forming units(CFUs)were counted.Bacterial quantity was expressed as the number of CFUs divided by the weight of each sample measured before and after collection.Results The elders showed a higher isolation frequency(93.33%)than children(45.45%)and youths(50.00%)which had statistically difference(P

AIM: To examine the effect of pretreatment with low-concentration of 11, 12-epoxyeicosatrienoic acid(EET) on myocardial ischemia/reperfusion injury in rats. METHODS: After tracheotomy, myocardial ischemia/reperfusion was produced by occlusion and release of the left anterior descending artery(LAD) of the rats. Ischemic preconditioning(IP) was made by two times of ischemia (5 min)/reperfusion (5 min). The experiment was conducted in three groups: control,IP and pretreatment with 11,12-EET(6.24?10 -8 mol/L), and each group was subdivided into two subgroups:A,the rats were subjected to ischemia (10 min)/reperfusion (10 min) and arrhythmias during the whole periods were monitored; The rats in B were subjected to ischemia (60 min)/reperfusion (30 min) and arrhythmias, cardiac funtion and myocardial infarction size were documented. RESULTS: Both IP and pretreatment with 11,12-EET could protect the heart against arrhythmias, cardiac disfunction and myocardial infarction. CONCLUSION: Pretreatment with 11,12-EET had protective effect on myocardium in case of ischemia/reperfusion, which was similar to ischemic preconditioning.