OBJECTIVE:To discuss the disputes in pharmaceutical care and the relevant preventive measures.METHODS:The disputes in pharmaceutical care and their reasons were analyzed,and the countermeasures were put forward.RESULTS&CONCLUSION:The countermeasures against disputes in pharmaceutical care are as follows.Firstly,the conception of“patient first and foremost”in pharmaceutical care should be established.The pharmaceutical service should be improved,and the mis?takes in dispensing and pricing of drugs should be prevented by establishing the particular system of two persons checking the prescription.Secondly,the drugs should be arranged in a scientific way,and the shortage of drugs should be prevented,while the control of drugs’expiration dates should be reinforced.Thirdly,the proper arrangement of human resources should be made.Last but not the least,the knowledge about rational drug use should be publicized,and the monitoring on ADR of drug should be developed.

CpG motifs are unmethylated oligodeoxynucleotides,and its core sequences are composed of unmethylated cytosine and unmethylated guanine.CpG motifs can induce the immune response by activating a variety of immune cells against diseases.The changes of CpG structure will increase the biological activities of CpG,and then enhance the therapeutic effect of CpG as the immuno adjuvant and the sensitizer of radiotherapy and chemotherapy in cancer treatment

OBJECTIVE:To prepare Qingyang tea bag and establish the standard of quality control.METHODS:The Flos Lonicera and Radix Glycyrrhize were identified by TLC.The content of chlorogenic acid,the main active component in the tea derived from Flos Lonicera,was determined by TLCs.RESULTS:Linearity was found in the range from1.2?g～4.8?g of chlorogenic acid,r=0.9993;the average recovery was99.50%and RSD=2.39%.CONCLUSION:The method was found to be simple,precise and may be used for the quality control of the preparation.

The peripheral blood T lymphocyte subsets and ConA-induced suppressor cell activity (ConA-SCA) were determined in 37 patients with primary nephritic syndrome(PNS). The results showed that the patients in initial onset had the abnormal number and function of T lymphocytes, which returned to a normal level with treatment with steroid and improvement of patients'condition. The indings suggest that the immunoregulatory imbalance of this disease may be a primary change and play an important role in pathogenesis of PNS.

To evaluate CT in the diagnosis of tuber culosis of the brain． CT findings in 95 cases with tuberculosis of t he bran confirmed clinically were analyzed retrospectively． Results: The CT detectability fate was 80%． The direct CT si ngs included exudation in basal system, meningitis, enhancement and intracranial tuberculosi s． The indirect signs had hydrocephalus infarction, atrophy calcification, periventricular hypo densith and subdural fluid accumulation etc． The most usual appearance was hydrocephalus． Conclusions: (1) It was helpful to diagnose when very two kinds of direct sings or a kind of dir ect sings and more than two kinds of indirect sings appeared． (2)The plain and contrast canning s hould be routinely used in tuberculosis of the brain．

To explore the opportunities and the challenges on continuing medical education of health technique cadres of armed police force,the article discussed the countermeasures of con-tinuing medical education of armed police force,in order to develop the work of continuing medical education of armed police force,which offered the methods and references for bringing up the ex-cellent health technique cadre of armed police force.

The principle and function of FluoViewTM FV500 laser scanning confocal microscope are expatiated.Suggestions are put forward in the selection of fluorescence probe,sample preparation,selection of parameters and environment requirment in using laser scanning confocal microscope.

Objective To investigate the effect of the Senlm of patients with congestive heart failure(CHF) on the apoptosis of endothelial progentior eelts(EPCs)and protective role of carvedil01.Method Totally 20 patientswithCHF、^mre enrolledfromDepartment ofCardiology ofthe FirstHospital Affdiated ofNanchangUniversity between June and August 2007.Serum WflflL isolatedfrom blood ofthe patients.1lononuelear cells were isolated from blood of20 healthy volunteers by density gradient centrifugation.Attaching cells wel-e harvested after culture for 7 relays and the EPCs were anyzea by flow cytometry and immunofluorescenee method.EPCs were divided into 4 groups:group A,cultured in sE-of volunteers;group B,cultured in serum of CHF;group C,cultured in leNlm of CFIF and Carvedilol;group D,cultured in,erum of CI-IF and metoproM,After euhure for 24 hours,annex.inV/PI wss used to investigate the apoptosis rate ofEPCs and the activity ofcllspafle 3 were exanlilled.ELISA Wills used to detect the level of flerum TNF-a of patients with CHF.Multiple group compalrion Wills conducted with variance analvsis and q test.Results The apoptosis rate and easpase 3 activity of EPCs in group B[(7.5 4-0.9)%v8.(3.0 4-0.25)]were significantly ineased compared with group A[(3.4±0.7)%VS.(1.37±0.2),P

Objective To investigate the retention level and its influencing factors for male nurses,and explore effective strategies to increase retention level of male nurses.Methods A sample of 610 male nurses from many hospitals in Tianjin were investigated with the self-designed demographic questionnaire,Retention Scale and Perceived Organization Support.The results underwent analysis.Results Male nurses' retention score was (20.71±4.00) points.Single factor analysis found that marriage,age,number of children,time engaged in nursing work,the working years in present position,the purpose of the work had statistically significant influence on retention scores of the male nurses.Multiple linear regression analysis showed that marriage,professional and technical titles,the purpose of work,work support and value identification were the predictive effect on the degree of the retention.Conclusions The retention of male nurses in Tianjin was in the medium level.Hospitals and all relevant departments should take effective measures,improve the professional sense of worth of the male nurses and job satisfaction,in order to enhance the man nurses' retention rate,promote the sustainable development of the nursing team construction.

OBJECTIVE:To optimize the decoction process of agkistrodon. METHODS:RP-HPLC with pre-column derivatiza-tion was adopted. With the contents of 4 main amino acids in agkistrodon as index,the decoction process(decocting times,heating time,water quantity and medicinal material crushing granularity) was optimized by orthogonal tests and verified. RESULTS:The optimal technology of the decoction process of agkistrodon was as 3 times of decoction,60 min of heating time,50 ml of water consumption for 0.90 g medicinal material and No.6 sieve. The results of verification test showed the total extraction of 4 kinds of amino acids was 72.68 mg/g(RSD=3.77%,n=3). CONCLUSIONS:The decoction technology can be used for the decoction pro-cess of agkistrodon,and it is stable and feasible.