In-stent restenosis after interventional treatment remains an unsolved and important clinical problem. The proliferation and migration of arterial smooth muscle cells are key events frequently followed by vascular restenosis after angioplasty and continuous inflammation may also be the other important factor for the restenosis after stenting. Study of the pathogenesis of restenosis may find some potentially novel therapeutic pathways for attenuating in-stent restenosis.

Objective:To solve the management problems of using mobile cart, PDA, flat-panel computer and other mobile devices in hospital, promote the evolution of mobile device management to intelligent management. Methods: Select the mobile device management system for hospital use based on their own characteristics.Results: Manage all the mainstream operating system and terminal equipment; manage the hardware and software of mobile terminal and assets in hospital.Conclusion: Improve the management, reduce the maintenance workload, save the cost of operation and maintenance.

The international meeting of the 10th anniversary of the AADO, convoked from Oct.8 to Oct. 10 in 2002 in Shanghai, was sponsored by AADO and organized by the two orthopedics departments of Changhai Hospital and Ruijin Hospital, Shanghai. The supporters of this meeting were Stryker Pacific, Stryker Trauma and Stryker China. There were 180 conventioneers who came from all over the mainland China, America, Germany, India, Japan, Hong Kong and Taiwan. They were clinical specialists in traumatology, managers from traffic departments and experts in vehicle design. 17 theses were selected for the meeting. The theme of the meeting was musculoskeletal trauma in developing countries with focus on road traffic injuries &geriatric fall injuries. Many experts in orthopedic trauma were invited to give presentations at the meeting on the theme.

Objective To evaluate the diagnosing and typing value of ultrasonography in adult congenital ureter cysts.Methods Ultrasonography findings were depicted of 29 cases with adult congenital ureter cysts confirmed by surgical and pathological examinations. Results In 29 cases of congenital ureter cyst, 14 occurred in right lateral, 12 in left lateral, and 3 in bilateral, totally being 32 cysts. The largest size of the cyst in diameter was (5.2) cm, and the smallest was (0.4) cm. Twenty-eight cases was diagnosed correctly by ultrasonography,in which the accuracy was (96.6)%(28/29). According to the sonographic patterns of size, shape, periodical change,1 case hydronephrosis, surgical and pathological results, 32 adult ureter cysts were divided into four types: type Ⅰ, papillary(12); type Ⅱ, round or ellipse(9);type Ⅲ, semilunar(4);and type Ⅳ, oblate(7). Conclusions Ultrasonographic appearance of adult ureter cyst is specific, and ultrasonography is of great value.

BACKGROUND:Non-hematopoietic cell of mesenchymal stem cells (MSCs) or mesenchymal progenitor cells can differentiate into bone,cartilage,muscle,tendon,fat and bone marrow matrix in vivo and in vitro,thus becoming very valuable seed cells source in the field of tissue engineering.OBJECTIVE:To construct the tissue engineered tendon withⅠcollagen-polyglycolic acid as a scaffold and bone marrow MSCs as seed cells,and to observe the effect of tissue engineered tendon on repairing achilles tendon defects. DESIGN,TIME AND SETTING:Randomized controlled animal experiment was performed in the Xiangya Medical College of Central South University in 2003.MATERIALS:Healthy adult rabbits,irrespective of genders,weighing 1.5-2.5 kg,were offered by Animal Center of Xiangya Medical College,Central South University. Polyglycolic acid were purchased from Shandong Weigao Group Kanglida Medical Products Co.,Ltd (KLD Medical).SD rats were sourced from Department of Experimental Animals,Central South University.METHODS:Rabbit bone marrow was extracted to harvest MSCs with centrrfugation and adherence method,then bone marrow MSCs were isolated and amplified. Tail tendon was extracted from SD rats and prepared into type Ⅰ collagen solution,which was mixed and suture cultured with polyglycolic acid to construct collagen-polyglycolic acid scaffold. Other bone marrow MSCs,which were not induced in vitro,were incubated on collagen-polyglycolic acid scaffold to construct tissue engineered rabbit tendon models,with those without cells serving as controls. Thirty rabbit angle skins were cut open to separate tendon and produce a 3-cm defect. Fifteen rats in the experiment group was repaired with tissue engineered tendon,which was previously prepared with autologous bone marrow MSCs,while fifteen rats in the control group was given Ⅰ collagen-polyglycolic acid scaffold.MAIN OUTCOME MEASURES:Effect of tissue engineered tendon on repairing achilles tendon defects in rabbit.RESULTS:No matter whether contains autologous bone marrow MSCs,type I collagen-polyglycolic acid scaffold transplanted into animals exhibited column gel shape by general observation. At 4 weeks following transplantation,cordlike tissues were seen in the transplantation site,polyglycolic acid suture was degraded. At 8 weeks,the tissue engineered tendon tissues were cordlike shaped,white,lustrous and dense. They were well connected with receptor tendon tissues in the experiment group,without adhesion to peripheral tissues. In the control group,the tissues were slender and adhered to peripheral tissues. CONCLUSION:Using typeⅠ collagen-polyglycolic acid as a scaffold and bone marrow MSCs as seed cells,tissue engineered tendon can dramatically promote the repair of achilles tendon defects.

Objective To observe the effects of saponins of Panax notoginseng (PNS) on the brain energy metabolism and mitochondrial respiratory function of oxidative damaged rat model mimic to Alzheimer disease (AD). Methods Oxidative damaged rat model mimic AD (AD group) was established by injection of dihydroxy fumaric acid (DHF) and FeCl3 ADP into the left lateral ventricle of rats. The contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), and phosphate creatine (Pcr) in brain were determined by high performance liquid chromatography (HPLC). The mitochondrial functions (R3, R4, P/O, and OPR) were observed by Clark oxygen electrode method. The difference in the above parameters in rats in the control group (injection of saline), model group, and administration group (injection of PNS) was observed. Results The levels of ATP, Pcr, and R3, R4, P/O, and OPR of rats in AD group were significantly lower than those in the control group (0.9% NaCl instead of DHF FeCl3 ADP), but those in PNS group were higher than those in the AD group. Conclusion PNS can improve brain energy metabolism and mitochondrial function of oxidative damaged rats, suggesting that PNS could be used for the treatment of AD.

AIM: To investigate the change of estrogen receptor (ER) in hippocampus, hypothalamus and pituitary of female rats exposed to psychological stress, and to illuminate the mechanism of dysfunction on ovarian reproductive endocrine function. METHODS: Sound, light and electricity were combined into a psychological stressful stimulus to induce female rat dysfunction on ovarian reproductive endocrine function. Immunohistochemical technique and image analysis were used to assess the expression levels of ER in hippocampus, hypothalamus and pituitary. RESULTS: When exposed to compound stressful stimulus of sound, light and electricity for 20 days in female rats, the expression levels of ER in hippocampus, hypothalamus and pituitary dropped. CONCLUSION: The decrease in estrogen receptor expression in hippocampus, hypothalamus and pituitary of female rats exposed to psychological stress may be one of the mechanisms of ovarian reproductive endocrine dysfunction.

Objective To evaluate the antibacterial activity of Galla chinensis etc.14 kinds of TCM against 112 strains of Staphylococcus aureus in vitro. Method Deterimination of antibacterial activity of Galla chinensis etc.against 112 strains of Staphylococcus aureus was performed by using new agar dilution method. Results The antibacterial efficiency of Galla chinensis, Forsythia suspensa and Acacia catechu (L.) Willd against 112 strains Staphylococcus aureus was good, and the MIC90 was 0.102, 0.244, 1.19 mg/mL respectively. Conclusion Galla chinensis etc. 14 kinds of TCM had strong antibacterial activity agsinst 112 srains of Staphylococcus aureus.

Objective To observe antibacterial efficiency of Prunus mume Sieb et Zucc against clinical isolates. Method Antibacterial activity of Prunus mume Sieb et Zucc against 308 strains of clinical isolates were determined by agar dilution method. Results MIC50 of Prunus mume Sieb et Zucc against staphylococcus aureus (112 strains), S.epidermidis (112 strains), and Enterococci (28 strains) were 0.72, 1.44, 0.72 mg/mL, and MIC90 were 1.44, 1.44, 0.72 mg/mL respectively. The MIC90 of Prunus mume Sieb et Zucc to Klebsiella pneumoniae and E. coli were 2.88, 1.44 mg/mL respectively. Conclusion Prunus mume Sieb et Zucc has good antibacterial activity against Gram positive cocci and some Gram negative bacilli.