OBJECTIVETo investigate necessity of the further processing for adsorptive resin material.

METHODSAccording to GB/ T 16886.4, coagulation tests, haematology tests, in vitro spontaneous platelet-adhesion test and complement activation tests were conducted respectively to assess the in vitro blood compatibility of adsorptive resin material.

RESULTSIn comparison with polypropylene, he adsorptive resin material appears to be a very slight activator to the endogenous blood coagulation system, it can inhibited the exogenous blood coagulation system very significantly (P < 0.01), it can adhere to fibrinogen, red blood cells, reticulocytes very significantly (P < 0.01), and platelet significantly (P < 0.05). The adsorptive resin material is also revealed to be a very significant activator (P < 0.01) to the complement system.

CONCLUSIONSThe in vitro blood compatibility assessment witnesses that it is very necessary for the adsorptive resin material to go through the certain special processing(such as the surface modification and the microencapsulation technology, etc) before its application to human being.

Rapid and accurate diagnosis of pathogens is the responsibility of the clinical microbiology lab.Effective implementation of this function requires standard procedure of specimen collection and processing.In order to improve the ability of infectious disease diagnosis,clinical microbiology laboratory should strengthen the communication with the clinicians,to improve the quality and quantity of specimens.Meanwhile,microbiology laboratory should learn some clinical knowledge and understand the demand and development of clinical practice.Clinical microbiology lab should emphasize patient-oriented and multidisciplinary close cooperation,for improvement of pathogen diagnosis level.

Objective To observe the repairing effect of hyaluronic acid hydrogel on rat articular cartilage defects .Methods One hundred and twenty SD rats were randomly divided into group A (natural repair group) ,group B (hyaluronic acid group) and group C (composite material group) ,36 rats was in each group and 12 rats were used to obtain cartilage cells .Full‐thickness articular os‐teochondral defects(2 .5 mm in diameter and 3 .0 mm in depth)were created mechanically in femoral trochlea of rats .Histological and biochemical method were used to evaluate the repairing effect of different material at different points(10 ,20 ,40 d) .Results Repairing effect of group C was best by histological and biochemical method at different points ,followed by group B ,and they were better than that of group A .Conclusion Hyaluronic acid hydrogel could provide cartilage cell proliferation for porous space truss structure ,and could carry cartilage cells at the same time .the cellular space truss structure have high surrounding tissue compatibil‐ity and biodegradation ,it is helpful for cartilage cell survival ,reproduction and division .

BACKGROUND:Survivin gene is the most potent anti-apoptosis factor, which can inhibit tumor cel proliferation and migration. It has become a hot topic related to cancer research in recent years.
OBJECTIVE:To explore the effect of Survivin gene silencing on the biological characteristics of G666-1 nasopharyngeal carcinoma cel s.
METHODS:siRNA sequence of Survivin was designed and synthesized and then transfected into G666-1 cel s using LipofectamineTM 2000. After Survivin gene silencing, Survivin mRNA and protein expressions were detected by RT-PCR and western blot assay, respectively. Expression of CD44+CD133+cel s was detected by flow cytometry. In addition, the effects of Survivin gene silencing on the proliferation, migration and invasion of G666-1 cel s were evaluated through cel counting kit-8 test, scratch healing assay and cel invasion assay.
RESULTS AND CONCLUSION:After Survivin gene silencing, the expressions of Survivin mRNA and protein were significantly reduced (P<0.05);the growth of G666-1 cel s was slowed dramatical y, indicating the proliferation ability was inhibited, and the migration and invasion abilities were both decreased significantly (P<0.05);the expression ratio of CD44+CD133+cel s was significantly lowered (P<0.05). These findings indicate that the proliferation, migration and invasion of G666-1 nasopharyngeal cancer stem cel s can be suppressed after Survivin gene silencing, and the proportion of CD44+CD133+cel s is reduced, thus indicating Survivin gene silencing may be a new effective target for treatment of nasopharyngeal carcinoma.

Magnetic mediated particles can be located within tumor volume through special ways are can be heated while exposed to an externally alternating magnetic field,thus kill the tumor cells.Compared with othen hyperthermia a major advantage of magnetic mediated hyperthermia is that of targeting tumor specially and selectively.The evidences of clinical research demonstrate that magnetic mediated hyperthermia will have a promising future and play an important role in tumor therapy.

The soluble antigen was isolated from pig retinal extract by precipitation with ammonium sulfate followed by molecular sieve. 50 ?g S antigen with equal volume complete Freund's adjuvant was injected into double hind foodpads of guinea pigs. Two weeks later, experimental autoimmune uveitis developed. Its pathologic feature is that a great number of lymphocytes infiltrated into chroid which was thickened gradually from anterior to posterior. Inflammatory variation was relative to disease phase. Ultrastructure pathology was characterized by swelling and necrosis in the inner and outer segments of photorecepter cells. This result suggests that S antigen has significant effect on inducing experimental autoimmune uveitis and the outer layer of retina is the sensitive target organ of S antigen.

AIM Protective action of TMP on hearts and brains under the condition of the acute hypoxia was studied. METHODS On the device of mice acute hypoxia ,ability of tolerancing hypoxia of mice was investigated,and superoxide dismutase(SOD) and malondialdehyde(MDA) and nitric oxide(NO) contents in hearts and brains were measured.RESULTS TMP could make the resisting hypoxia action in mice strengthen significantly,and the action had a does dependent increase. TMP made SOD contents in hearts and brains in does dependent increase;MDA contents in hearts and brains were not notable in low does ,but TMP could decreased MDA contents in hearts and brains in middle and high does ( P

Objective To measure the sizes of the left and right internal jugular veins (IJV) and transverse sinuses (TS) with three dimensional contrast‐enhanced MRA(3D CE‐MRA) ,and discuss the correlation of cerebral venous sinus and IJV .Methods One hundred and ten patients without vasculopathy and blood flow abnormalities were selected for 3D CE‐MRA ,55 cases for each gender .The images were taken three‐dimensional reconstruction on work‐station by different software .The normal IJV and TS was measured and the statistical comparison between the bilateral IJV and TS was analyzed .Results (1)The inside diameter of IJV re‐duced in turn at the measurement point of J1 ,J2 and J3 .The internal diameter of the right side of IJV was broader than left one ,and there was significant difference between the size of left and right side (P<0 .05) in these measurements .The internal diameter of the right side of TS was broader than left one ,there was difference between the sizes of the left and right of TS (P<0 .05) .(2)The proportion of R>L ,R

In this paper, some study methods of pharmacokinetic, the explanation ways of data and experimental methods of transdermal therapeutic system(TTS) were described. The advancement of modern science accelerates the development of pharmacokinetic of TTS and makes more progress in this field.

[Objective] To evaluate the efficacy and safety of quadruple therapy based on moxifloxacin and bismuth in the treatment of Helicobacter pylori eradication failure.[Methods] A prospective,randomized,comparative study was conducted in which 136 eligible patients were randomly divided into the extended quadruple group (66 patients,and the quadruplex therapies are Pectin bismuth 100 mg tid + lansoprazole 30 mg bid + amoxicillin 1000 mg bid + moxifloxacin 400 mg qd) and the classical quadruple group (70 patients,and the quadruple therapies are colloidal pectin bismuth 100 mg tid+ lansoprazole 30 mg bid +tetracycline tablet 750 mg Bid + metronidazole tablets 400 mg bid combination) and were under treatment for 14 days.One month after the treatment,endoscopy,rapid urease test,and 13-carbon/ 14-carbon urease breath test were used to evaluate the adverse effects and adherence of the drugs in the two groups respectively.[Results] In the extended quadruple group,among 60 patients who had completed the final treatment and follow-up survey,55 were successfully treated,with eradication rates of (83.3％,55/66)and 91.7％ (55/60) according to intention-to-treat (ITT) and Per-protocol (PP) respectively.In the classic quadruple group,64 patients completed the final treatment and follow-up survey.Among them,37 cases were successful,and the eradication rates according to ITT and PP remedy are 52.9％ (37/70) and 57.8％ (37/64) respectively.The difference was statistically significant (P ＜ 0.001).Adverse drug reactions and compliance monitoring showed no significant difference between the two groups (P ＞ 0.05).[Conclusion] The quadruple therapy based on moxifloxacin and bismuth displays a higher eradication rate than that of the classical bismuth quadruple therapy on the first-time elimination failure of Helicobacter pylori while shows no difference in safety consideration,thus can serve as remedial therapy for the primary Hp eradication failure.