Objective To investigate the clinical features of nonresponsive Kawasaki disease by intravenous immunoglobulin. Methods The clinical data of 163 hospitalized patients of Kawasaki disease from June 2008 to February 2013 were analyzed retrospectively based on the response to high intravenous immunoglobulin (IVIG) therapy. All patients were divided into responsive group and nonresponsive group. The clinical characteristics and biochemical index were compared in two groups. Results The nonresponsive group had more frequently experienced in coronary artery lesion, cervical lymph node enlargement, sterile pyuria and higher levels of neutrophils, CRP, AST, ALT, TBIL and lower levels of albumin, high density lipoprotein, serum sodium concentration than the responsive group (P < 0.05). No differences was found in the age, sex, duration of fever at initial treatment, clinical manifestations complete or incomplete and WBC, CK-MB, PLT, ESR between the two groups (P > 0.05). Conclusion It might be useful in predicting nonresponse to IVIG therapy in children with Kawasaki disease in more frequently experienced coronary artery lesion , cervical lymph node enlargement, sterile pyuria and higher levels of neutrophils, CRP, AST, ALT, LDH, TBIL and lower levels of albumin, high density lipoprotein, serum sodium concentration.

Objective To investigate the effects of hypoxia inducible factor-2?(HIF-2?) on the proliferation and invasion of hepatocellular carcinoma cell line SMMC-7721 under the anoxia condition.Methods Lipofectamine-mediated HIF-2? oligodeoxynucleotide(ODN) was transfected into SMMC-7721 cells and the expressions of HIF-2? mRNA and protein were detected to evaluate the transfection.The proliferative and adhesive activity was determined by methyl thiazolyl tetrazolium(MTT) method and the invasive activity was determined by a transwell cell culture chamber method.Results The expressions of HIF-2? mRNA and protein were inhibited significantly by HIF-2? antisense oligodeoxyribonucleotide(ASODN)(P0.05).Conclusion The blockage of HIF-2? expression inhibits the adhesive and invasive activity of SMMC-7721cells,and has no evident impact on their proliferation.

Objective To investigate the of dural reconstruction plates for treatment of humeral intercandylar fracture.Methods The 52 cases with humeral intercandylar fracture were treated with dural reconstruction plates internal fixation.Watched the clinical efficacy Postoperative in outpatient,and guide early exercise of elbow.Result Using Elbow Scoring Standard System to assess:16 cases of C1,excellent and good in 14 cases,fair in 2 cases,and 87.5％ excellent and good results were obtained.Twenty-four cases of C2,excellent and good in 20 cases,fair in 4 cases,and 83.3％ excellent and good results were obtained.Twelve cases of C3,excellent and good in 6 cases,fair in 2 cases,and poor in 4 case,and 50.0％ excellent and good results were obtained.Fifty-two cases were followed up,excellent and good in 40 cases,fair in 8 cases,and poor in 4 case,and 76.9％ excellent and good results were obtained.Conclusion Dural reconstruction plates internal fixation was the relatively effective method for treatment of humural intercandylar fracture.

Objective To explore the levels of serum hepatitis B surface antigen (HBsAg) in different clinical stages of hepatitis B virus(HBV) infection and their correlation with serum HBV DNA. Methods Five hundred and seventy-five patients with HBsAg-positive and without antiviral therapy were enrolled in this study. The patients were classified into six group:IT group (immune tolerant phase, 120 patients), EPH group (hepatitis B e antigen positive hepatitis, 110 patients), LR group (low replicative phase, 90 patients), ENH group (hepatitis B e antigen negative hepatitis, 110 patients), LC group (liver cirrhosis stage, 85 patients) and HCC group (hepatocellular carcinoma, 60 patients). Serum HBsAg and HBV DNA levels were quantified and analyzed through statistics. Results The levels of serum HBsAg in IT group, EPH group, LR group, ENH group, LC group and HCC group were (4.58 ± 0.40), (4.12 ± 0.50), (2.60 ± 0.68), (3.31 ± 0.27), (2.82 ± 0.57) and (3.03 ± 0.39) lg U/ml respectively, and there was significantly different among the different phase (P<0.01). The levels of serum HBsAg in IT group, EPH group, LR group, ENH group, LC group and HCC group had positive correlation with serum HBV DNA level, and the correlation coefficients were 0.627, 0.579, 0.134, 0.317, 0.159 and 0.224 respectively. Conclusions The levels of serum HBsAg is significantly different in the different clinical phase of HBV infection, and the tendency of the correlation between serum HBsAg and serum HBV DNA gradually weakens in general.

Ojective To study the endothelial mechanism of simulated weightlessness on the reactivity of pulmonary artery. Methods Experiments were conducted using 32 Wistar male rats, which were randomly divided into 2 groups: tail-suspended group and control group, 16 rats for each group. Tail-suspension for 14 days was used to simulate the effects of microgravity. The changes in responses of pulmonary artery to several vascular active agents were determined by using in vitro vessel rings perfusion technique. And the changes were determined again after the endothelium of pulmonary artery was removed. Results Compared with the control group, the contractile responses of pulmonary artery to potassium chloride (68mmol/L) and phenylephrine (PE, 10 -7 ~10 -5 mmol/L) were markedly diminished after 14 days tail-suspension, while the dilatory responses to acetylcholine (Ach, 10 -8 ~10 -6 mmol/L) were markedly enhanced. The vascular reactivity of endothelium-removed pulmonary artery rings to PE and sodium nitroprusside (SNP) did not change after 14 days tail-suspension. Conclusion The contractile responsiveness is attenuated and the dilatory responsiveness is enhanced in the rats' pulmonary arteries after simulated weightlessness. That the pulmonary artery endothelium changes in structure or function after simulated weightlessness might be an important cause of changing in the reactivity of pulmonary artery.

Objective To evaluate the interventional chemoembolization combined with proton radiotherapy in the treatment of hepatocellular carcinoma (HCC) accompanied with cancerous thrombus in the main portal vein. Methods Interventional chemoembolization combined with proton radiotherapy was performed in 46 patients of HCC accompanied with cancerous thrombus in the main portal vein. The proton radiotherapy was broke up into several fractions. The patients were treated with interventional chemoembolization and, alternatively, with proton radiotherapy. The short-term effectiveness and radiation reaction were evaluated. The survival rate was followed up. Results The effective rate was 91.3% . Disappearance of cancerous thrombus in the main portal vein was seen in 45.6% of patients. The liver functions were well restored, with a remarkable reduction in AFP. No acute or delayed radiation-induced hepatic damage or radiation hepatopathy occurred during the course of and after radiotherapy. The survival rate at 1, 6, 12 and 24 months was 100%, 89.1%, 52.2% and 21.4% respectively, with a median survival period of 17.6 months. Conclusion For the patients of HCC accompanied with cancerous thrombus in the main portal vein, interventional chemoembolization combined with proton radiotherapy is an effective, safe and newly-developed therapy.

Objective To study the effects of simulated weightlessness on eNOS expression in the pulmonary artery in rat, and to investigate the mechanism of changed reactivity of pulmonary artery during simulated weightlessness. Methods The experiments were conducted in 16 male Wistar rats, and they were divided into 2 groups: suspension group and control group, 8 rats for each. Tail-suspension (-30?) was carried out for 14d to simulate weightlessness. eNOS protein expression and NO content in pulmonary artery were determined using Western blot analysis and nitrate enzyme-reduction method, respectively. Results Compared with control group, eNOS expression in the rat pulmonary artery increased significantly during simulated weigtlessness. The relative optical density as a result of scanning was 247?2.60, and that of the control was 141?1.72. There were significant differences between the two groups (P

Objective To investigate the effects of dexamethasone on prevention of apoptosis in pulmonary tissues of rats after simulated weightlessness. Methods Tail suspension was used to simulate weightlessness. Thirty Sprague-Dawley male rats were divided randomly into 3 groups: control group, tail suspension for 7 days group, and dexamethasone treatment after suspension group. TUNEL technique was used to detect cell apoptosis in pulmonary tissues. Results The rate of apoptotic cells in the lung of rats suspended for 7 day was higher than that of control group(P

Objective To investigate the effects of dexamethasone on inducible nitric oxide synthase (iNOS) in pulmonary tissues of tail-suspended rats simulating weightlessness. Methods Twenty Sprague-Dawley male rats were randomly divided into 2 groups: tail-suspended for 7 days (n=10) without treatment and dexamethasone treatment group (n=10). Tail-suspension was used to simulate weightlessness. The expression of iNOS in the pulmonary tissue was examined by Western blot and immunohistochemistry. Results The results showed that the level of iNOS in the pulmonary tissue of treatment group was decreased significantly(P

Golgi protein 73(GP73) was closely correlated with primary hepatic carcinoma(PHC). GP73 had a higher sensitivity and specificity than alpha fetal protein(AFP), so GP73 had a great diagnostic value for early PHC. Although there were many related research reports in the domestic and foreign, their conclusions were inconsistent. This article reviewed these studies results.