The quality of sleep has a great relationship with health and working efficiency. The result of sleep stage classification is an important indicator to measure the quality of sleep, and it is also an important way to diagnose and treat sleep disorders. In this paper, the method of detrended cross-correlation analysis (DCCA) was used to analyze sleep stage classification, sleep electroencephalograph signals, which were extracted from the MIT-BIH Polysomno graphic Database randomly. The results showed that the average DCCA exponent of the awake period is smaller than that of the first stage of non-rapid eye movement (NREM) sleeps. It is well concluded that the method of studying the sleep electroencephalograph with this method is of great significance to improve the quality of sleep, to diagnose and to treat sleep disorders.
Electroencephalography ; Humans ; Signal Processing, Computer-Assisted ; Sleep Stages

When people are walking, they will produce gait signals and different people will produce different gait signals. The research of the gait signal complexity is really of great significance for medicine. By calculating people's gait signal complexity, we can assess a person's health status and thus timely detect and diagnose diseases. In this study, the Jensen-Shannon divergence (JSD), the method of complexity analysis, was used to calculate the complexity of gait signal in the healthy elderly, healthy young people and patients with Parkinson's disease. Then we detected the experimental data by variance detection. The results showed that the difference among the complexity of the three gait signals was great. Through this research, we have got gait signal complexity range of patients with Parkinson's disease, the healthy elderly and healthy young people, respectively, which would provide an important basis for clinical diagnosis.
Aged ; Gait ; Healthy Volunteers ; Humans ; Parkinson Disease ; physiopathology ; Reference Values ; Signal Processing, Computer-Assisted ; Walking

Aged ; Angiofibroma ; metabolism ; pathology ; Desmin ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Inflammation ; Leiomyosarcoma ; pathology ; Neoplasms, Muscle Tissue ; metabolism ; pathology ; Vimentin ; metabolism ; Vulvar Neoplasms ; metabolism ; pathology

Adult ; Aged ; Ascitic Fluid ; chemistry ; Blood Gas Analysis ; methods ; Carbon Dioxide ; analysis ; blood ; Female ; Humans ; Hydrogen-Ion Concentration ; Liver Cirrhosis ; blood ; Male ; Middle Aged ; Oxygen ; analysis ; blood

OBJECTVEThis study evaluates the expression of interleukin-18 (IL-18) and hypoxia-inducible factor (HIF)-lα in rat periodontitis model exposed to normoxia and chronic intermittent hypoxia (CIH) environments. The possible correlation between periodontitis and obstructive sleep apnea-hypopnea syndrome (OSAHS) was also investigated. Methods: Thirty-two Sprague-Dawley (SD) rats were randomly assigned into four groups: normoxia control, normoxia periodontitis, hypoxia control, and hypoxia periodontitis groups. The periodontitis models were established by ligating the bilateral maxillary second molars and employing high-carbohydrate diets. Rats in hypoxia control and hypoxia periodontitis groups were exposed to CIH treatment mimicking a moderately severe OSAHS condition. All animals were sacrificed after eight weeks, and the clinical periodontal indexes were detected. The levels of IL-18 and HIF-1α in serum and gingival tissues were determined using enzyme-linked immunosorbent assay (ELISA). The correlation between attachment loss (AL) and the levels of IL-18 and HIF-lα in hypoxia periodontitis group was evaluated.

RESULTSThe levels of IL-18 and HIF-lα in hypoxia periodontitis group were significantly higher than that in normoxia periodontitis and hypoxia control groups (P<0.05). Furthermore, the levels of IL-18 and HIF-lα in serum (r-0.792, r=0.753, P<0.05) and gingival tissues (r-0.817, r=0.779, P<0.05) were positively correlated with AL.

CONCLUSIONCIH could aggravate the destruction of periodontal tissues, which is correlated with IL-18 and HIF-lα levels.

Animals ; Disease Models, Animal ; Enzyme-Linked Immunosorbent Assay ; Gingiva ; Hypoxia ; Hypoxia-Inducible Factor 1, alpha Subunit ; Interleukin-18 ; Periodontal Index ; Periodontitis ; Rats ; Rats, Sprague-Dawley ; Serum ; Sleep Apnea, Obstructive

Microbial genome reduction and modification are important strategies for constructing cellular chassis used for synthetic biology. This article summarized the essential genes and the methods to identify them in microorganisms, compared various strategies for microbial genome reduction, and analyzed the characteristics of some microorganisms with the minimized genome. This review shows the important role of genome reduction in constructing cellular chassis.
Genes, Essential ; genetics ; Genetic Engineering ; Genome, Microbial ; genetics ; Synthetic Biology ; methods

Along with the high prevalence of obesity in children and adolescents,obesity can lead to im-paired glucose tolerance,dyslipidemia,elevation of blood pressure,the risk of metabolic syndrome,diabetes,and cardiovascular and cerebrovascular diseases gradually increases.As end-event cardiovascular disease(CVD)is hardly present in childhood,there is a critical need for early markers to assess,predict,and treat the children who are vulnerable to developing CVD.For mitigating the incidence of future CVD through early detection and pre-vention programs,this paper reviews the relationship between endothelial dysfunction and immunologic dysfunc-tion,chronic inflammation,oxidative stress,insulin resistance.And it also provides a critical overview on early-noninvasive measurements about endothelial function in obese children.

The Sprouty protein can inhibit cel proliferation and induce apoptosis by specific interventions in the signaling pathways of tumor cells, such as receptor tyrosine kinase signaling pathway. The Sprouty protein, which is often associated with abnormal cell proliferation and transformation, is down-regulated in many tumors, stimulating the initiation and progression of tumors. The Sprouty protein exhibits potential for developing effective treatment against cancer. This paper reviews research progress on Sprouty protein in tumor.

With the thorough research on cognitive impairment in old age,vascular cognitive inpairment (VCI) has become a hot research field of neuroscience in recent years.Yet,the VCI-related pathogenic mechanism currently remains unknown.Therefore,the reliable and stable animal models are needed to reveal the pathogenesis and pathophysiologic changes of VCI in order to provide a theoretical basis for early warning,diagnosis and treatment of VCI.The commonly used animal models now have their own advantages and disadvantages,the results have greater differences.This article reviews the commonly used VCI animal models,related cognitive behavior changes,and change characteristics of histopathology in recent years,and the reasonable animal models of VCI according to different research objectives are investigated preliminarily.

Objective To investigate the changes of the pulmonary surfactant protein-D (SP-D) in serum and cerebrospi-nal lfuid in children with viral encephalitis (VE). Methods The levels of SP-D in serum and cerebrospinal lfuid were detected by a double-antibody sandwich enzyme-linked immunosorbent assay and compared in thirty children with VE in acute and con-valescent phases and in 12 children without VE. Results The levels of SP-D in serum and cerebrospinal lfuid between groups of VE acute phase and convalescent phase and no VE were statistically signiifcant (F=103.58,118.15, all P<0.01). The levels of SP-D in serum and cerebrospinal lfuid in children with VE in acute phase and in convalescent phase were signiifcantly lower than children without VE (P<0.01). The levels of SP-D in serum and cerebrospinal lfuid in children with VE in convalescent phase were all signiifcantly higher than those in acute phase (P<0.01). In children with VE, the level of SP-D in cerebrospinal lfuid was weakly correlated negatively with the count of nucleated cells. Conclusions SP-D might be involved in the pathogenesis in VE. The detection of SP-D in serum and cerebrospinal lfuid has a certain value for diagnosis of VE.