Objective This study aims to explore antihypertension and heart protective effect of acupuncture on SHR rats through the observation of blood pressure, cardiac ultrasound and pathology examination of SHR rats after needling the Zusanli (ST 36) and Taichong (LR 3). Methods A total of 14 SHR rats (10 weeks) were randomly divided into two groups:6 for model group and 8 for acupuncture group, another 6 SD rats (10 weeks) were used as the control group. SHR rats in the acupuncture group were fixed in the holder, and then they exposed both lower limbs for needling both sides Zusanli (ST 36) and Taichong (LR 3), and then they retained needles for 20 minutes per time with four weeks. The other two groups were fixed in the holder without needling. Blood pressure was examined each week. LVSs, LVDd, LVPWs, LVPWd were measured and recorded by cardiac ultrasound in the day after the whole course of acupuncture. LVM, LVMI, RWT were calculated. The hearts of rats were dissected and fixed in formalin for heart pathology detection after doing the cardiac ultrasound. Results After acupuncture treatment, compared with model group, the systolic blood pressure (SBP) of the third week (178.38 ± 9.47 mmHg vs. 190.00 ± 13.90 mmHg) and the fourth week (167.96 ± 23.47 mmHg vs. 195.47 ± 11.36 mmHg) of acupuncture group significantly decreased (P<0.01). The diastolic pressure (DBP) of the third week (139.33 ± 13.20 mmHg vs. 159.56 ± 12.89 mmHg) and the fourth week (132.92 ± 18.02 mmHg vs. 165.61 ± 13.36 mmHg) of acupuncture group significantly decreased (P<0.01). The LVSs (0.96 ± 0.07 vs. 1.28 ± 0.24), LVPWs (1.15 ± 0.08 vs. 1.68 ± 0.19) of the acupuncture group were significantly lower than those of the model group (P<0.01). The LVM (0.51 ± 0.12 vs. 0.84 ± 0.17) and LVMI (14.96 ± 1.53 vs. 23.65 ± 5.04) of acupuncture group were significantly lower than those of the model group (P<0.01). Histopathologic examination of the heart of the model group showed chronic inflammatory granulation tissue hyperplasia and fibrosis in myocardial outer membrane, but the acupuncture group showed no obvious changes in the heart tissue. Conclusions Acupuncture treatment on Zusanli (ST 36) and Taichong (LR 3) could effectively control the left ventricular hypertrophy by decreasing the SBP and DBP , prevent cardiac remodeling, and protect myocardial cells.

In light of the objective circumstances and its subjective conditions, our hospital, a grade A, second tier urban general hospital, made the right choice of reorienting itself towards community health service after making a careful assessment of the current situation. In the course of the reorientation, our hospital mainly focused on such tasks as change of concepts, readjustment of its structure and functions, establishment of the service mode of the family physician, delivery of the "six in one" service in the form of a team, and implementation of standard management. Such efforts proved to be fruitful and won the approval of the community. On the basis of this, our hospital tried hard to exploit to the full its favorable conditions as a second tier hospital in providing community health service and called for attention and solution to such issues as the pricing system, the fundraising and compensation mechanism, cost accounting, and reform of the personnel system in the delivery of community health service.

In recent years, neuro-electrophysiological techniques, as objective evaluation methods, have been gradually used in func-tional evaluation, guiding clinical rehabilitation programs and judging prognosis after stroke. They were proved to judge the integrity of cen-tral nervous system and conduction function and evaluate neuromuscular function. Nerve conduction, needle electromyography, H reflex, F wave, sympathetic skin response, motor-evoked potentials, somatosensory-evoked potentials, brain stem auditory-evoked potentials and sur-face electromyography were reviewed in this article.

Objective To establish an high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method for determination of glucosamine hydrochloride and its related substances in glucosamine and indometacin enteric-coated capsules.Methods Separation was carried out on a Inertsil C8 Column (250 mm ×4.6 mm,5μm) with a mobile phase composed of acetonitrile-water(70∶30) at a flow rate of 1.0 mL/min and the column temperature was 25℃.Results The linear ranges of calibration curve was 25 -312.5 μg/mL(r=0.9991, n=6).The average recovery was 98.0%, and the relative standard deviation was 1.3%( n =5 ) .The glucosamine hydrochloride was stable with acid destruction, but unstable with alkali, heat and strong light destruction.Conclusion The method is simple, accurate and reproducible for determination of glucosamine hydrochloride and related substances.

Objective To investigate the effect of early acupuncture at Huatuo jiaji points on striatalβ-endorphin and dynorphin levels in rats with post-stroke limb spasm.Methods Seventy SD rats were randomized into group A (normal) of 9 rats and group B (sham operation) of 10 rats. After a model of post-stroke limb spasm was made in the remaining rats, they were randomized into groups C (model), D (acupuncture at Huatuo jiaji points) and E (baclofen). Group D received acupuncture at Huatuo jiaji points and group E, an oral gavage of baclofen tablets. After seven days of treatment, striatalβ-endorphin and dynorphin levels were neasured by radioimmunoassay.Resultsβ-endorphin levels increased significantly in groups C, D and E compared with groups A and B (P<0.01) and also in groups D and E compared with group C (P<0.01,P<0.05). There was no statistically significant difference inβ-endorphin levels between groups D AND E (P>0.05). Dynorphin levels increased significantly in groups C, D and E compared with groups A and B (P<0.05,P<0.01). There was no statistically significant difference in dynorphin levels between groups D and E (P>0.05).Conclusions Acupuncture at Huatuo jiaji points can increase striatalβ-endorphin levels but not change striatal dynorphin levels, which conforms to the relationship between enkephalin and spasm, and improve the animal’s spasticity.

Objective To analysis the frequency of 9 bp deletion polymorphism of mitochondrial DNA(mt DNA)among Miao population in three geographical regions of Guizhou.Methods Polymerase chain reaction and polyacrylamide gel electrophoresis (PCR-PAGE)were used in the study of mtDNA 9 bp deletion polymorphism,DNA sequence was used to verify the results.Results Each group who come from Basa,Shajin,Longjie in Guizhou,the frequency of 9 bp deletion of mtDNA value as 19.15%(9/47), 42.5%(17/40)and 29.55%(13/43).Short polymorphic frequency difference was statistically significant between Basa and Shajin groups(P<0.05).Conclusion The frequencies of 9 bp deletion of mtDNA are relatively high in Miao ethnic group in Guizhou. There are certain differences of the delegation frequencies among these groups.

[Objective] To discuss the clinical effect of Jianpi Qingwei Decoction on chronic erosive gastritis(CEG). [Method] Choose the said patients 68 cas-es, randomly divide them into treatment group(n=38) and control one(n=30).The treatment group take the self-made Jianpi Qingwei Decoction for treat-ment;the control one, lansoprazole and col oid pectinBi. Both take 4w as a course;after 1 course, compare their cure effect and side effects;fol ow up the re-currence. [Result] In the treatment group, 22 cases were cured, 13 better, 3 uncured, the total effective rate 92.10%;for the control one, they were respective-ly 13,11,6 and 80.00%;by comparison, their difference had statistical meaning;there ’s no recurrence fol owing up for half year. [Conclusion] Jianpi Qingwei Decoction has marked cure effect and little side effects on CEG, without easy recurrence, worth promotion.

Under certain conditions,stress can bring physiological,behavioral or psychological response,thus affect human health and cause diseases.Available intervention methods must be adopted to eliminate or relieve internal environment disorder from severe stress.Studies of stress intervention are of great importance to maintain and promote physical and psychological health.According to the different characteristics,stress intervention methods can be divided into psychological and behavioral.This review presented the advance of stress intervention methods.

Magnetic resonance spectroscopy(MRS)is a non-invasive technique permitting in vivo determination of biochemical parameters within brain tissue. This paper reviewed MR spectroscopic studies performed on patients with central nervous system tumors and discussed the impact of such studies on the tumor diagnosis and management.

Since the first description of tension-free herniorrhaphy and the developement of prosthetic ma-terial for inguinal hernia, many techniques have come and gone. We should deploy the idea of evidence-based medicine and clinical practice guideline, according to the pathogenetie condition and medical condi-tion, in order to make the best treatment perseription for patient under the condition of nomudization.