1.Expression and function of voltage-gated Na+ channel isoforms in rat sinoatrial node.
Xin HUANG ; Ai-Qun MA ; Pei YANG ; Yuan DU ; Yu-Tao XI ; Tao GENG
Journal of Southern Medical University 2007;27(1):52-55
OBJECTIVETo detect the expression of voltage-gated Na(+) channel (NaCh) isoforms in rat sinoatrial node and explore their functions.
METHODSExpressions of NaCh isoforms Nav1.1, Nav1.2, Nav1.3, Nav1.5, Nav1.6 and Nav1.7 in the rat sinoatrial node were detected by immunohistochemistry. The functional roles of the NaChs were tested by observing the effect of tetrodotoxin, a specific blocker of NaChs, on the intrinsic heart rate of isolated rat working heart.
RESULTSThe tetrodotoxin- sensitive neuronal isoforms Nav1.1, Nav1.6 and Nav1.7 as well as the tetrodotoxin-resistant cardiac isoform Nav1.5 were present in the rat sinoatrial node, and the neuronal isoforms were more abundant than Nav1.5 (P<0.05). The selective blockade of tetrodotoxin-sensitive isoforms (presumably Nav1.1, Nav1.6 and Nav1.7) by 100 nmol/L tetrodotoxin scarcely affected the intrinsic heart rate (0.5-/+2.9%, P>0.05) while blockade of tetrodotoxin-resistant isoform (presumably Nav1.5) by 2 micromol/L tetrodotoxin resulted in an obvious decline in the intrinsic heart rate (22.1-/+2.1%, P<0.001).
CONCLUSIONSNav1.1, Nav1.5, Nav1.6 and Nav1.7 are all present in rat sinoatrial node. Although neuronal isoforms are more abundant, Nav1.5 seems to contribute more to activity of the sinoatrial node.
Animals ; Heart Rate ; drug effects ; physiology ; Immunohistochemistry ; Ion Channel Gating ; drug effects ; physiology ; Male ; NAV1.1 Voltage-Gated Sodium Channel ; NAV1.5 Voltage-Gated Sodium Channel ; NAV1.6 Voltage-Gated Sodium Channel ; Nerve Tissue Proteins ; biosynthesis ; Protein Isoforms ; biosynthesis ; Rats ; Sinoatrial Node ; drug effects ; metabolism ; physiology ; Sodium Channels ; biosynthesis ; Tetrodotoxin ; pharmacology
2.Structure-based assessment of disease-related mutations in human voltage-gated sodium channels.
Weiyun HUANG ; Minhao LIU ; S Frank YAN ; Nieng YAN
Protein & Cell 2017;8(6):401-438
Voltage-gated sodium (Na) channels are essential for the rapid upstroke of action potentials and the propagation of electrical signals in nerves and muscles. Defects of Na channels are associated with a variety of channelopathies. More than 1000 disease-related mutations have been identified in Na channels, with Na1.1 and Na1.5 each harboring more than 400 mutations. Na channels represent major targets for a wide array of neurotoxins and drugs. Atomic structures of Na channels are required to understand their function and disease mechanisms. The recently determined atomic structure of the rabbit voltage-gated calcium (Ca) channel Ca1.1 provides a template for homology-based structural modeling of the evolutionarily related Na channels. In this Resource article, we summarized all the reported disease-related mutations in human Na channels, generated a homologous model of human Na1.7, and structurally mapped disease-associated mutations. Before the determination of structures of human Na channels, the analysis presented here serves as the base framework for mechanistic investigation of Na channelopathies and for potential structure-based drug discovery.
Animals
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Calcium Channels, L-Type
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chemistry
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genetics
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metabolism
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Channelopathies
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genetics
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metabolism
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Humans
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Mutation
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NAV1.1 Voltage-Gated Sodium Channel
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chemistry
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genetics
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metabolism
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NAV1.5 Voltage-Gated Sodium Channel
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chemistry
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genetics
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metabolism
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NAV1.7 Voltage-Gated Sodium Channel
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chemistry
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genetics
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metabolism
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Protein Domains
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Rabbits
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Structure-Activity Relationship
3.Neuronal signaling in central nervous system.
Acta Physiologica Sinica 2011;63(1):1-8
A new method of axon recording through axon bleb has boosted the studies on the functional role of central nervous system (CNS) axons. Using this method, we have revealed the mechanisms underlying the initiation and propagation of the digital-mode signal, all-or-none action potentials (APs), in neocortical pyramidal neurons. Accumulation of the low-threshold Na(+) channel subtype Na(v)1.6 at the distal end of the axon initial segment (AIS) determines the lowest threshold for AP initiation, whereas accumulation of the high-threshold subtype Na(v)1.2 at the proximal region of the AIS promotes AP backpropagation to the soma and dendrites. Through dual recording from the soma and the axon, we have showed that subthreshold membrane potential (V(m)) fluctuations in the soma propagate along the axon to a long distance and probably reach the axon terminals. Paired recording from cortical neurons has revealed that these V(m) changes in the soma modulate AP-triggered synaptic transmission. This new V(m)-dependent mode of synaptic transmission is called analog communication. Unique properties of axonal K(+) channels (K(v)1 channels) may contribute to shaping the AP waveform, particularly its duration, and thus controlling synaptic strength at different levels of presynaptic V(m). The level of background Ca(2+) may also participate in mediating the analog signaling. Together, these findings enrich our knowledge on the principles of neuronal signaling in the CNS and help understand how the brain works.
Action Potentials
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physiology
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Animals
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Axons
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physiology
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Central Nervous System
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cytology
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physiology
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Humans
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Membrane Potentials
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physiology
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NAV1.2 Voltage-Gated Sodium Channel
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physiology
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NAV1.6 Voltage-Gated Sodium Channel
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physiology
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Neocortex
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cytology
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physiology
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Patch-Clamp Techniques
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Pyramidal Cells
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physiology
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Sodium Channels
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physiology
4.Advances in the studies on the molecular and genetic aspects of epilepsy.
Xu WANG ; Tao WANG ; Ming-xiong YUAN ; Mu-gen LIU ; Qing WANG
Acta Academiae Medicinae Sinicae 2005;27(3):388-393
Epilepsy is one of the most common and debilitating neurological diseases that affects more than 40 million people worldwide. Genetic factors contribute to the pathogenesis of epilepsy. Molecular genetic studies have identified 15 disease-causing genes for epilepsy. The majority of the genes encode ion channels, including voltage-gated potassium channels KCNQ2 and KCNQ3, sodium channels SCN1A, SCN2A, and SCN1B, chloride channels CLCN2, and ligand-gated ion channels CHRNA4, CHRNB2, GABRG2, and GABRA1. Interestingly, non-ion channel genes have also been identified as epilepsy genes, and these genes include G-protein-coupled receptor MASS1/VLGR1, GM3 synthase, and proteins with unknown functions such as LGI1, NHLRC1, and EFHC1. These studies make genetic testing possible in some patients, and further characterization of the identified epilepsy genes may lead to the development of new drugs and new treatments for patients with epilepsy.
Chloride Channels
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genetics
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Epilepsies, Myoclonic
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genetics
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Epilepsy
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genetics
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Epilepsy, Absence
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genetics
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Humans
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KCNQ2 Potassium Channel
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genetics
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KCNQ3 Potassium Channel
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genetics
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NAV1.1 Voltage-Gated Sodium Channel
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NAV1.2 Voltage-Gated Sodium Channel
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Nerve Tissue Proteins
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genetics
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Sodium Channels
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genetics
5.Progress in molecular genetics of epilepsy.
Chinese Journal of Medical Genetics 2002;19(6):505-507
Epilepsy is a group of disorders characterized by recurrent seizures. The etiologies of idiopathic epilepsy commonly have a genetic basis. Gene mutations causing several of the inherited epilepsies have been mapped. In this review, the authors summarize the available information on the genetic basis of human epilepsies and epilepsy syndromes, emphasizing how genetic defects may correlate with the pathophysiological mechanisms of brain hyperexcitability and gene defects can lead to epilepsy by altering multiple and diverse aspects of neuronal function.
Epilepsy
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genetics
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Humans
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KCNQ2 Potassium Channel
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Mutation
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NAV1.1 Voltage-Gated Sodium Channel
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Nerve Tissue Proteins
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genetics
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Potassium Channels
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genetics
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Potassium Channels, Voltage-Gated
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Receptors, Nicotinic
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genetics
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Research
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trends
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Research Design
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Sodium Channels
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genetics
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Voltage-Gated Sodium Channel beta-1 Subunit
6.Nav1.8 and Nav1.9 mRNA expression in rat trigeminal ganglion at different interval after molar extraction.
Lei ZHANG ; Hong-Chen LIU ; Dong-Sheng WANG
Chinese Journal of Stomatology 2009;44(5):301-303
OBJECTIVETo observe the expression and function of extraction.
METHODSReal-time reverse transcription PCR paralleled with vitro-established cRNA standard curves was applied to measure the expression of Nav1.8, Nav1.9 at 30 min, 2 h, 1 d, 3 d and 6 d respectively after extraction of rat right mandibular molars. The right mandibular molars were used as control.
RESULTSBoth Nav1.8 and Nav1.9 mRNA in right trigeminal ganglion showed little change after 30 min, and increased slowly after 2 h. Nav1.8, Nav1.9 mRNA expressions increased by 27% and 24.5% respectively compared to the left trigeminal ganglion after 3 d, reaching the highest level (P < 0.05), and then the expressions began decreasing from 6 d.
CONCLUSIONSThe pain caused by molar extraction is related to the up-regulation of expressions of sodium channels protein Nav1.8 and Nav1.9 mRNA, indicating the participation of sodium channels in regulations of peripheral tissue pain after molar extraction.
Animals ; Male ; NAV1.8 Voltage-Gated Sodium Channel ; NAV1.9 Voltage-Gated Sodium Channel ; Neuropeptides ; metabolism ; Pain, Postoperative ; metabolism ; RNA, Messenger ; genetics ; Rats ; Rats, Wistar ; Sodium Channels ; metabolism ; Tooth Extraction ; Trigeminal Ganglion ; metabolism
8.Expression of Kir2.1, SCN5a and SCN1b channel genes in mouse cardiomyocytes with various electric properties: patch clamp combined with single cell RT-PCR study.
Hong-Yan LUO ; Hua-Min LIANG ; Xin-Wu HU ; Ming TANG
Acta Physiologica Sinica 2012;64(1):82-86
This study is to explore a new method of investigating molecular basis for electrophysiological properties of early fetal cardiomyocytes. Single embryonic cardiomyocytes of mouse early developmental heart (E10.5) were obtained by a collagenase B digestion approach. After recording spontaneous action potential using whole cell patch clamp technique, the single cell was picked by a glass micropipette, followed by a standard RT-PCR to explore the expression levels of several ion channel genes. Three phenotypes of cardiomyocytes were demonstrated with distinct properties: ventricular-like, atrial-like, and pacemaker-like action potentials. Ventricular-like and atrial-like cells were characterized with much negative maximum diastolic potential (MDP) and a higher V(max) (maximum velocity of depolarization) compared to pacemaker-like cells. MDP of ventricular-like cells was the most negative. In parallel, stronger expression of SCN5a, SCN1b and Kir2.1 were observed in ventricular-like and atrial-like cells compared to that of pacemaker-like cells, where Kir2.1 in ventricular-like cells was the most abundant. Cardiomyocytes with distinct electrophysiological properties had distinct gene expression pattern. Single cell RT-PCR combined with patch clamp technique could serve as a precise detector to analyze the molecular basis of the special electrophysiological characteristics of cardiomyocytes.
Animals
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Electrophysiological Phenomena
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Female
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Fetus
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Male
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Mice
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Myocytes, Cardiac
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metabolism
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physiology
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NAV1.5 Voltage-Gated Sodium Channel
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genetics
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metabolism
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Patch-Clamp Techniques
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Potassium Channels, Inwardly Rectifying
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genetics
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metabolism
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Real-Time Polymerase Chain Reaction
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Voltage-Gated Sodium Channel beta-1 Subunit
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genetics
;
metabolism
9.Long QT syndrome gene diagnosis by haplotype analysis.
Jiang-fang LIAN ; Chang-cong CUI ; Xiao-lin XUE ; Chen HUANG ; Han-bing CUI ; Hai-zhu ZHANG
Chinese Journal of Medical Genetics 2004;21(3):272-273
OBJECTIVEThree long QT syndrome(LQTS) pedigrees were brought together for genetic diagnosis by using short tandem repeat(STR) markers.
METHODSGenomic DNA was extracted from blood samples. STR markers (D7S1824, D7S2439, D7S483, D3S1298, D3S1767, D3S3521) in or spanning the HERG and SCN5A gene were amplified; the haplotype analysis for LQTS was performed.
RESULTSClinical diagnosis showed that 15 are LQTS patients (3 died) and 11 are probable patients. Linkage analysis showed that LQTS patients are linked with the SCN5A gene in family 1, HERG is linked with the disease in family 2 and 3. Fourteen gene carriers were identified, 2 patients and 7 probable patients were excluded.
CONCLUSIONLinkage analysis using STR markers can serve as useful tool for presymptomatic diagnosis.
ERG1 Potassium Channel ; Ether-A-Go-Go Potassium Channels ; Female ; Genetic Linkage ; Haplotypes ; Humans ; Long QT Syndrome ; genetics ; Male ; NAV1.5 Voltage-Gated Sodium Channel ; Pedigree ; Potassium Channels ; genetics ; Potassium Channels, Voltage-Gated ; Sodium Channels ; genetics ; Tandem Repeat Sequences