Background: Obstetric complications always are the risk factors which impact on pregnant women, especially no having care and treatment on time. Objectives: (1) To determine the rate of obstetric complications in some communes of Hoai Duc district, Ha Tay province. (2) To identify some factors which may have impact obstetric complications in the community. Subjects and method: Conducting a cross \ufffd?sectional survey with 363 women randomly chosen for sampling from the list of 1.530 women of reproductive age having terminated their pregnancy dressed for a six \ufffd?month period (from 1 October 2004 to 31 March 2005) in 10 communes of Hoai Duc district, Ha Tay province. Results: The obstetric complications rate was 9.9%. Obstetric complications recorded under the research often had the following forms: hemorrhage (94.4%), infection and pre \ufffd?eclampsia/eclampsia, each of them (2.8%). obstetric complications regularly happened with women of over 40 years old (20%). Those complications were closely linked to vaginal bleeding symptom during pregnant period with p<0.05. Conclusion: Obstetric complication rate was 9.9%, mostly hemorrhage. There was close relation between obstetric complication with vaginal bleeding symptom during pregnant period.
Obstetric Labor Complications ; Residence Characteristics ;

Aims: A suitable medium and cultivation parameters have an important role in the improvement of the production of pyocyanin pigment by Pseudomonas aeruginosa microorganism. The study aimed to optimize culture conditions and medium components for maximal pyocyanin production in a recombinant strain P. aeruginosa PS39-phzMS created in previous research. In addition, the process of extraction of pyocyanin was also investigated to select a proper applied solvent for recovering a high amount of pyocyanin as well as its quality. Methodology and results: The pyocyanin purification has based on solvent. Among six tested solvents for extracting pyocyanin out of bacterial broth, two out of six recovered a significant amount of pyocyanin, namely dichloromethane and chloroform, in which chloroform showed a higher pyocyanin yield (25.27 ± 1.02 µg/mL) than dichloromethane (20.26 ± 0.88 µg/mL). The thin-layer chromatography (TLC) of the extracted pyocyanin illustrated a similar to pure pyocyanin with Rf of 0.72 and no mark of other impurity metabolites. The UV-Vis spectra showed a similar peak at 520 nm with pure pyocyanin and the highest peak at 274 nm. Each single culture parameter was studied for the maximal production of pyocyanin. Next, a pyocyanin-producing GM medium was modified on the base of the KingA to find the relative capacity to biosynthesize high pyocyanin yield in P. aeruginosa PS39-phzMS. The results showed that pyocyanin production was the highest in optimal culture conditions, at 30 °C, pH 8, 120 h and agitation of 200 rpm. In the combination of culture condition with the GM, pyocyanin was created at the highest amount of 49.57 µg/mL. Conclusion, significance and impact of study Based on the obtained results of the study, a pyocyanin-producing procedure was optimized, which suggests a promising application to scale-up pyocyanin production by the P. aeruginosa PS39-phzMS.