1.Poria cocos ethanol extract and its active constituent, pachymic acid, modulate sleep architectures via activation of GABA(A)-ergic transmission in rats.
Vikash Kumar SHAH ; Sam Shik NA ; Myong Soo CHONG ; Jae Hoon WOO ; Yeong Ok KWON ; Mi Kyeong LEE ; Ki Wan OH
Journal of Biomedical Research 2015;16(3):84-92
Poria cocos is a well-known traditional Chinese traditional medicine (TCM) that grows around roots of pine trees in China, Korea, Japan, and North America. Poria cocos has been used in Asian countries to treat insomnia as either a single herb or part of an herbal formula. In a previous experiment, pachymic acid (PA), an active constituent of Poria cocos ethanol extract (PCE), increased pentobarbital-induced sleeping behaviors. The aim of this experiment was to evaluate whether or not PCE and PA modulate sleep architectures in rats as well as whether or not their effects are mediated through GABA(A)-ergic transmission. PCE and PA were orally administered to individual rats 7 days after surgical implantation of a transmitter, and sleep architectures were recorded by Telemetric Cortical encephalogram (EEG) upon oral administration of test drugs. PCE and PA increased total sleep time and non-rapid eye movement (NREM) sleep as well as reduced numbers of sleep/wake cycles recorded by EEG. Furthermore, PCE increased intracellular chloride levels, GAD65/67 protein levels, and alpha-, beta-, and gamma-subunits of GABA(A) receptors in primary cultured hypothalamic neuronal cells. These data suggest that PCE modulates sleep architectures via activation of GABA(A)-ergic systems. Further, as PA is an active component of PCE, they may have the same pharmacological effects.
Administration, Oral
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Animals
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Asian Continental Ancestry Group
;
China
;
Cocos*
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Electroencephalography
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Ethanol*
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Eye Movements
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Glutamate Decarboxylase
;
Humans
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Japan
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Korea
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Medicine, Chinese Traditional
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Neurons
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North America
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Pinus
;
Poria*
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Rats*
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Receptors, GABA-A
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Sleep Initiation and Maintenance Disorders
2.Pachymic Acid Enhances Pentobarbital-Induced Sleeping Behaviors via GABA(A)-ergic Systems in Mice.
Vikash Kumar SHAH ; Jae Joon CHOI ; Jin Yi HAN ; Mi Kyeong LEE ; Jin Tae HONG ; Ki Wan OH
Biomolecules & Therapeutics 2014;22(4):314-320
This study was investigated to know whether pachymic acid (PA), one of the predominant triterpenoids in Poria cocos (Hoelen) has the sedative-hypnotic effects, and underlying mechanisms are mediated via gamma-aminobutyric acid (GABA)-ergic systems. Oral administration of PA markedly suppressed locomotion activity in mice. This compound also prolonged sleeping time, and reduced sleep latency showing synergic effects with muscimol (0.2 mg/kg) in shortening sleep onset and enhancing sleep time induced by pentobarbital, both at the hypnotic (40 mg/kg) and sub-hypnotic (28 mg/kg) doses. Additionally, PA elevated intracellular chloride levels in hypothalamic primary cultured neuronal cells of rats. Moreover, Western blotting quantitative results showed that PA increased the amount of protein level expression of GAD65/67 over a broader range of doses. PA increased alpha- and beta-subunits protein levels, but decreased gamma-subunit protein levels in GABA(A) receptors. The present experiment provides evidence for the hypnotic effects as PA enhanced pentobarbital-induced sleeping behaviors via GABA(A)-ergic mechanisms in rodents. Taken together, it is proposed that PA may be useful for the treatment of sleep disturbed subjects with insomnia.
Administration, Oral
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Animals
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Blotting, Western
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Cocos
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gamma-Aminobutyric Acid
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Hypnotics and Sedatives
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Locomotion
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Mice*
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Muscimol
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Neurons
;
Pentobarbital
;
Poria
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Rats
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Receptors, GABA-A
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Rodentia
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Sleep Initiation and Maintenance Disorders