1.Low-density lipoprotein protects Vibrio vulnificus-induced lethality through blocking lipopolysaccharide action.
Kwang Hyun PARK ; Jong Suk KIM ; Young Rae LEE ; Young Jae MOON ; Hyun HUR ; Yun Hee CHOI ; Cheon Hyeon KIM ; Uh Hyun KIM ; Eun Kyung SONG ; Wan Hee YOO ; Chang Seop LEE ; Byeong Soo KIM ; Sung Ho LEE ; Phil Youl RYU ; Myung Kwan HAN
Experimental & Molecular Medicine 2007;39(5):673-678
Lipoprotein plays a role in the host defense against bacterial infection, and its serum level has been demonstrated to be an important prognosis factor of survival. We have previously demonstrated that LDL directly inactivates the hemolytic activity of Vibrio vulnificus cytolysin (VVC) in vitro. The object of this study was therefore to examine whether the LDL-mediated inactivation of VVC leads to protection against lethal infection of V. vulnificus in vivo, using wild and VVC-deficient V. vulnificus strains. Unexpectedly, we found that LDL protects mouse lethality induced by VVC-deficient as well as wild V. vulnificus strain. We also demonstrated that LDL blocks V. vulnificus LPS-induced lethality in mice. These results suggest that LDL preferentially act on endotoxin rather than exotoxin in the protection against V. vulnificus-induced mice lethality.
Animals
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Disease Models, Animal
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Female
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Humans
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Lipopolysaccharides/*antagonists & inhibitors
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Lipoproteins, LDL/*pharmacology
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Mice
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Mice, Inbred ICR
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Perforin/antagonists & inhibitors/genetics
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Vibrio Infections/prevention & control
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Vibrio vulnificus/*drug effects/genetics/*pathogenicity
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Virulence/drug effects/genetics/physiology
Result Analysis
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