OBJECTIVETo isolate and identify the chemical constituents from Verbena officinalis.

METHODThe compounds were isolated by means of chromatography and the structures were determined on the basis of physical and spectral analysis.

RESULTFour compounds were isolated and identified as apigenin (I), 4'-hydroxywogonin (II), verbenalin (III) and hastatoside (IV).

CONCLUSIONCompounds I and II were obtained from the genus for the first time.

Apigenin ; chemistry ; isolation & purification ; Flavanones ; chemistry ; isolation & purification ; Iridoid Glycosides ; Iridoids ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Verbena ; chemistry

OBJECTIVETo establish a HPLC method for determination of ursolic acid in dried aerial part of Verbena officinalis.

METHODThe column used was a Kromasil C18 (4.6 mm x 250 mm) packed with a 5 microns stationary phase. The mobile phase consisted of methanol-sodium phosphate buffer [monobasic sodium phosphate (MW = 119.98) 1.7997 g and phosphoric acid (85%) 1.02 mL, combined and brought the total volume of 1,000 mL with water] (89:11); the mobile phase was maintained at a flow-rate of 0.8 mL per minute; the column was maintained at 40 degrees C; the DAD detector was set at 210 nm.

RESULTSThe liner range was 0.251-10.04 micrograms (r = 1.0000). An average recovery of 98.1% (n = 6) was obtained with a RSD of 1.0%.

CONCLUSIONThe method is simple, accurate and suitable for the qualify control.

Chromatography, High Pressure Liquid ; methods ; Plant Components, Aerial ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control ; Triterpenes ; analysis ; Verbena ; chemistry