2.Quantitative immunohistochemical study on the effect of dark condition on the vasoactive intestinal peptide immunoreactivity in the suprachiasmatic nucleus of the rat.
Song Sup LEE ; Myung Young LEE ; Byung Lan LEE ; Choong Ik CHA ; Sa Sun CHO ; Wang Jae LEE ; Kwang Ho LEE ; Sang Ho BAIK ; Ka Young CHANG
Korean Journal of Anatomy 1992;25(4):350-360
No abstract available.
Animals
;
Rats*
;
Suprachiasmatic Nucleus*
;
Vasoactive Intestinal Peptide*
3.Effect of androgen on corpus cavernosal strip in the rabbit.
Young Chan KIM ; Hyung Ki CHOI ; Young Soo AHN ; Kyung Hwan KIM
Korean Journal of Urology 1992;33(4):717-721
Testosterone is intimately related to normal sexual functioning. However most impotent patients have a normal level of circulating free testosterone. In this study. the effects of testosterone and dihyrotestosterone were observed using the isolated corpus cavernosal strip from the rabbit. Pre-treatment of testosterone and dihyrotestosterone had no effect on the contraction induced by a-adrenergic agonist. Furthermore both of them had no synergistic effect on the relaxation produced by acetylcholine or vasoactive intestinal polypeptide. Further studies about the central effect are needed for clarifying the action mechanism of androgen.
Acetylcholine
;
Erectile Dysfunction
;
Humans
;
Male
;
Relaxation
;
Testosterone
;
Vasoactive Intestinal Peptide
5.The Effect of Vasoactive Intestinal Peptide on Cord Blood CD34 (+) Cells.
Mi Kyong YEO ; Hwa Young BAE ; Min Kyu HUR ; Jae Sun RA ; In Seob HAN ; Min Keong KIM ; Soon Ki KIM ; Sang Kyu PARK
Korean Journal of Pediatric Hematology-Oncology 2003;10(2):262-268
PURPOSE: We investigated the expression of vasoactive intestinal peptide (VIP), VIP receptor 1 (VPAC1), VIP receptor 2 (VPAC2) genes in the human umbilical cord blood CD34 cells, and the ability of VIP to stimulate human primitive as well as monopotent hematopoietic progenitors. METHODS: We isolated RNA from umbilical cord blood CD34 cells, and then performed RT-PCR, and sequencing. The umbilical cord blood CD34 cells were cultured with the various concentrations of VIP for burst-forming unit of erythrocyte (BFU-E), colony-forming unit of granulocyte/monocyte (CFU-GM), colony-forming unit of graulocyte/erythrocyte/monocyte/megakaryocyte (CFU-GEMM), and colony-forming unit of megakaryocyte (CFU-Mk). RESULTS: The RNA coding for VPAC1 was detected in human umbilical cord blood CD34 cells. VIP significantly stimulated the growth of CFU-GEMM and CFU-Mk. CONCLUSION: The present results suggest that VIP is an important neuropeptide in the early proliferation of human primitive as well as megakaryocyte progenitors.
Clinical Coding
;
Erythrocytes
;
Fetal Blood*
;
Humans
;
Megakaryocyte Progenitor Cells
;
Megakaryocytes
;
Myeloid Progenitor Cells
;
Neuropeptides
;
Receptors, Vasoactive Intestinal Peptide
;
RNA
;
Stem Cells
;
Vasoactive Intestinal Peptide*
6.Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells.
Cha-Xiang GUAN ; Chang-Qing ZHANG ; Xiao-Qun QIN ; Zi-Qiang LUO ; Fu-Wen ZHOU ; Xiu-Hong SUN
Acta Physiologica Sinica 2002;54(2):103-106
To investigate the influence of vasoactive intestinal peptide (VIP) on chemotaxis of bronchial epithelial cells (BECs). Rabbit chemotactic migration of primary BEC was assessed in a blind-well Boyden chamber. Radioimmunoassay and radio-ligand affinity analysis were used for determining VIP secretion and vasoactive intestinal peptide receptor (VIPR) expression. The results showed: (1) the method for determining chemotaxis of BECs by using insulin as chemotactic factor was stable and reproducible (r=0.9703, P<0.01). (2) VIP (0.001-1 micromol/L) elicited chemotaxis of BECs which was substantial and concentration-dependent. The effects of VIP were inhibited by W-7 and H-7 (P<0.01). (3) Heat stress enhanced the secretion of VIP (P<0.01) and upregulated the expression of VIPR on BECs (P<0.05). These results indicate that VIP in the lungs may play an important role in the repair of damaged epithelium, accelerating restoration of the airway to its normal state. Calmodulin and protein kinase C may be involved in the signal transduction of VIP effects.
Animals
;
Bronchi
;
cytology
;
Cells, Cultured
;
Chemotaxis
;
drug effects
;
physiology
;
Epithelial Cells
;
drug effects
;
physiology
;
Female
;
Insulin
;
pharmacology
;
Male
;
Rabbits
;
Receptors, Vasoactive Intestinal Peptide
;
biosynthesis
;
Vasoactive Intestinal Peptide
;
pharmacology
7.Quantitative variation of vasoactive intestinal polypeptide and its receptor in rat liver during development.
Li WANG ; Cheng-Wei TANG ; Chun-Hui WANG ; Xian LI
Acta Physiologica Sinica 2005;57(3):379-383
Accumulated data have suggested that vasoactive intestinal polypeptide (VIP) and corresponding receptor (VIPR) are involved in the development of hematopoietic stem cells and liver growth. In the present study, radioimmunoassay, biomolecular interaction analysis and reverse transcriptation polymerase chain reaction were used to quantify VIP, VIPR and detect the subtype of VIPR in rat liver during development. VIP concentration of liver in fetal or neonatal rats was significantly lower than that of teens or adult rats (P<0.05). The binding capacities of VIPR in liver of immature rats were much greater than that of the adult rats (P<0.05). The tendency of change in VIP concentration was contrary to that of the binding capacity of VIPR in the liver of rats during development. VIPR-1 was expressed in rat liver in all phases of development. These results may be of benefit to the understanding of the mechanisms of liver growth and fetal liver hemopoiesis shift.
Animals
;
Animals, Newborn
;
Liver
;
growth & development
;
metabolism
;
RNA, Messenger
;
metabolism
;
Rats
;
Rats, Sprague-Dawley
;
Receptors, Vasoactive Intestinal Peptide
;
metabolism
;
Vasoactive Intestinal Peptide
;
metabolism
8.Effect of vasoactive intestinal peptide receptor antagonist VIPhybrid on the development of form deprivation myopia in chicks.
Ping-bao WANG ; Hua WANG ; Shuang-zhen LIU ; Jing-jing JIANG
Journal of Central South University(Medical Sciences) 2008;33(8):669-675
OBJECTIVE:
To investigate the effect of regulation of VIPhybrid, an unselective antagonist of vasoactive intestinal peptide receptors (VIPR), on the formation and development of form deprivation myopia (FDM) in chick and the expression of protein and mRNA of VIP on the retina and choroids of in chicks.
METHODS:
Seventy-two 1-day-old yellow healthy leghorn chicks were assigned into 6 groups (12 in each group). Eyes in Group I were covered on the right as a blank control group. Eyes in GroupII were those eyes having been injected with 20 microL saline into vitreous cavity and then covered as a negative control group. Eyes in GroupIII,IV and V were injected with 20 microL VIPhybrid with low (3*10(-12) mol/L), middle (3*10(-10) mol/L) and high (3*10(-8) mol/L) dosage into vitreous cavity and then covered as experimental groups. The above groups had been continuously covered for 1 week. Eyes in Group VI were uncovered and uninjected as a normal control group. Diopter was detected using retinoscopic refraction. The eyeball axis was determined using ophthalmological ultra-A. The expression of protein and mRNA of VIP on retina-choroids-sclera were investigated by SP immunohistochemistry staining and RT-PCR.
RESULTS:
Form deprivation for 1 week induced high myopia eyes and elongated eyeball axis in GroupI and GroupII, and there was no difference between the 2 groups (P>0.05). The diopter and eyeball axis were significantly reduced in Group III, IV, and V as compared with Group I and II (P<0.01), but the diopter was higher and the eyeball axis was longer than those of Group VI. The diopter and eyeball axis had negative correlation with the concentration gradient of VIPhybrid. The expressions of protein and mRNA of VIP in Group III, IV, and V were down-regulated as compared with those of Group I and I I(P<0.01)and also down-regulated with the increase of concentration of VIPhybrid.
CONCLUSION
VIPhybrid can decrease the development of FDM in chicks, which may provide a new pathway for drug therapy of myopia in human beings.
Animals
;
Animals, Newborn
;
Chickens
;
Myopia
;
etiology
;
metabolism
;
RNA, Messenger
;
biosynthesis
;
genetics
;
Receptors, Vasoactive Intestinal Peptide
;
antagonists & inhibitors
;
Recombinant Fusion Proteins
;
pharmacology
;
Retina
;
metabolism
;
Vasoactive Intestinal Peptide
;
biosynthesis
;
genetics
9.Mechanism of vasoactive intestinal polypeptide-induced catecholamine secretion from the rat adrenal medulla.
Dong Yoon LIM ; Jae Bong HEO ; Cheol Hee CHOI ; Geon Han LIM ; Yong Gyoon LEE ; Song Hoon OH ; Il Sik KIM ; Jong In KIM
The Korean Journal of Physiology and Pharmacology 1998;2(4):443-454
The present study was attempted to investigate the effect of vasoactive intestinal polypeptide (VIP) on secretion of catecholamines (CA) and to establish whether there is the existence of a noncholinergic mechanism in adrenomedullary CA secretion from the isolated perfused rat adrenal gland. The perfusion into an adrenal vein of VIP (3 X 10-6 M) for 5 min or the injection of acetylcholine (ACh, 5.32 X 10-3 M) resulted in great increases in CA secretion. Tachyphylaxis to releasing effect of CA evoked by VIP was not observed by the repeated perfusion. The net increase in adrenal CA secretion evoked by VIP still remained unaffected in the presence of atropine or chlorisondamine. However, the CA release in response to ACh was greatly inhibited by the pretreatment with atropine or chlorisondamine. The releasing effects of CA evoked by either VIP or ACh were depressed by pretreatment with nicardipine, TMB-8, and the perfusion of Ca2+-free medium. Moreover, VIP- as well as ACh-evoked CA secretory responses were markedly inhibited under the presence of (Lys1, Pro2.5, Arg3.4, Tyr6)-VIP or naloxone. CA secretory responses induced by ACh and high K+ (5.6 X 10-2 M) were potentiated by infusion of VIP (3 X 10-6 M for 5 min). Taken together, these experimental results indicate that VIP causes CA release in a fashion of calcium ion-dependence, suggesting strongly that there exists a noncholinergic mechanism that may be involved in the regulation of adrenomedullary CA secretion through VIP receptors in the rat adrenal gland, and that VIP may be the noncholinergic excitatory secretagogue present in the chromaffin cells.
Acetylcholine
;
Adrenal Glands
;
Adrenal Medulla*
;
Animals
;
Atropine
;
Calcium
;
Catecholamines
;
Chlorisondamine
;
Chromaffin Cells
;
Naloxone
;
Nicardipine
;
Perfusion
;
Rats*
;
Receptors, Vasoactive Intestinal Peptide
;
Tachyphylaxis
;
Vasoactive Intestinal Peptide
;
Veins
10.Immunohistochemical observations on the changes of autoimmune innervation of the human vas deferens after vasectomy.
Korean Journal of Urology 1991;32(5):774-780
Immunohistochemical study have been made to examine the autonomic innervation of the human vas dererens following vasectomy one to 7 years previously. Samples from sites on the proximal (testicular) and distal (urethral) sides of the original vasectomy have been compared with control specimens as to the arrangement and distribution of autonomic nerves containing vasoactive intestinal polypeptide (VIP), catecholamine, substance-P and enkephalin. In contrast with tissues from the urethral portion and from controls, the testicular specimens revealed a marked reduction in the catecholeminergic innervation of the muscular layer. In addition VIPergic nerves distributed at the subepithelial layer were nearly absent from the testicular side of the vas deferens. The degrees of denervation were independent of the obstructive interval between vasectomy and vasectomy reversal. Substance-P and enkephalin containing nerves were rarely found from both sides of the vas deferens. Therefore, these findings suggest that the consequences of denervation of vas deferens may play an important role in those patients in whom infertility persists despite evidence of satisfactory mechanical continuity achieved by vasectomy reversal.
Autonomic Pathways
;
Denervation
;
Enkephalins
;
Humans*
;
Infertility
;
Vas Deferens*
;
Vasectomy*
;
Vasoactive Intestinal Peptide
;
Vasovasostomy