A prevalence study was conducted on Trichomonas vaginalis infection among female sex workers attending the Reproductive Health and Wellness Center of Angeles City, Pampanga in the Central Luzon region of the Philippines. Polymerase chain reaction (PCR) using T. vaginalis-specific primers TV3⁄7 was utilized to detect T. vaginalis in vaginal swabs from the study population. The lower limit sensitivity of T. vaginalis detection by the PCR assay was found to be one trichomonad. The overall prevalence in 377 women was 9.55%. More than half of the study subjects are 23-27 years old. However, the largest proportion of positive cases was found among subjects 18-22 years old, making it the age group with the highest T. vaginalis prevalence (12.84%).