In 2002-2003 year period at HCM City Blood Transfusion and Hematology 8 cases of umbilical cord blood transplantation were performed.including 2 cases of lymphoid acute leukemia, 3 cases of myeloid acute leukemia, 3 cases of beta thalassemia. Among them, there were a case of HLA- identical blood and 7 cases of HLA-dismatched blood. After 36 months, 61,90% of cases were recovered survivals and after 24 months 71,43%
Stem Cell Transplantation ; Epidemiology ; Vietnam ; Cord Blood Stem Cell Transplantation

Blood screening voluntary donor in HCMC. We investigated 47,621 samples of blood. Results: HbsAg (+): 7.15%, antiHCV: 3.11%, anti HIV: 0.29; VDRL (+) 1.08%, Malaria (+): 0.27%; abnormal antibodies: 0.20%.
Blood Donors ; Hepatitis C Antibodies ; Hepatitis B Surface Antigens ; Anti-HIV Agents

203 products of total blood and 203 products of the erythroprecipitin undergone the quality control during 1/200-27/9/2000. The results have shown that the blood volume of 4.92% products was lower than 250 ml and level of hemoglobin of 1% products was lower than 22g among 203 controlled products of the total blood; volume of 2.11% products was less than 125ml and of 12.67% products was more than 125 ml; the level of hemoglobin of 100% products was higher than 22 g and of 80% products was higher than 25g, the hematocrite of 4.22% products was higher than 0.75 l/l among 142 controlled products of the erythroprecipitin. The conclusion: products, in generally obtained the ISO 9002.
Quality Control ; blood ; Blood Platelets ; Erythrocytes

We performed 10 cases of autologous peripheral blood stem cell transplantation without cryoprevervation in 11/1996 and 12/1997 12. Patients were diagnosed of AML, ALL, CML and non Hodgkin’s lymphoma. The median age of the patients was 34 years (range 18-43). Peripheral blood stem cell mobilizing regiments consisted of Daunorubicine (50 mg/ m2/d, d1-d5) cytarabine and etoposide in 3 CML patients and high dose cyclophosphamide in the seven other patients, followed by G-CSF starting when an ANC< 0.5x 109/l until the day before the last leukapheresis. When a WBC count reached 4x109/l leukapheresis was started and continued for 3 consecutive days, as soon as the third collection was finished Melphalan was given as a conditioning regimen, 42 hours after giving Melphalan, the collected PSCs which were preserved at +40C (90 hours after giving Melphalan, the collected PSCs which were preserved at + 40C were reinfused through a central Hickman’s catheter. GM-CSF was given during the nadir period until the an ANC > 1x109/l for 3 consecutive days. The median number of mononucleated cells and CD34+ cell were 5x108/kg respectively. All platelet count > 20 x 109/l was 13 days and 20 days following transplantation, all patients are still in CR and alive with the median follow-up of 8 months.
Transplantation ; blood ; Hematologic Diseases

The study was carried out on 98 patients who needed to be transfused regularly. Phenotyped red blood cells of groups were used for this purpose. Only immunogenic antigens of the common blood groups were included in this study, such as Rh, MNSs, Duffy and Kidd systems. The highest quantity of units transfused depassed the number 44. After a period of thirteen months, there was no antibody detected. But in the other hand, we found 4 cases of immune antibodies in patients who previously received multiply transfusions of non phenotyped blood. These antibodies belong to Rh, Duffy and Kidd systems which are highly immunogenic. These results showed the need and importance of performing adequate compatibility testing for multiply transfused patients and the benefit of using phenotyped blood for those who need regular transfusions.
Phenotype ; Blood transfusion

The blood transfusion is necessary while the donor’s marrow has not been growed yet. The transfused blood products must assure completely the safety principles for disease transmission, the blood products is not infected the MCV and removed the leucocyte usually transfused after 4- 12 weeks of transplantation with the suitable dosage for individuals. Condensed platalet is transfused after 6-12 weeks of transplantation. It should maintain the amount of thrombocyte in the peripheral blood within 50-100x109/lit, the dose is different from one patient to another.
Blood transfusion ; Bone marrow ; Transplantation

The bone marrow transplantation is a most modern technique and applied in Vietnam to treat the malignant hematological diseases. This paper introduced the bone marrow heterotransplantation and the autologous peripheral blood stem cell transplantation. 3 patients (1 male; 2 female) received transplantation (bone marrow fluid was extracted from patient’s brothers) for the treatment of the chronic myeloid leukemia and severe thalassemia and 17 patients received the autologous peripheral blood stem cell transplantation for treatment of acute myeloid leukemia, chronic myeloid leukemia, acute lyphoid leukemia and lymphoma. The results have shown that the longest survival duration was 42 months after the autologous peripheral blood stem cell transplantation. The patients had a normal life such as learning, working and social activities. After 2 months of transplantation, the average disease free duration of 10 patients with AML and 6 patients with ALL were 27 months, and 30-40 months, respectively. This was an great encouragement.
Bone Marrow Transplantation ; Bone marrow ; Bone and bones

A 33 years old female with acute myeloid leukemia was treated with induction chemotherapy of 7-3-5 protocol (Cytarabine, Daunorubicine, Etoposide). On day 15 of chemotherapy; 15 days from the first blood transfusion and 1 day from the last, she developed high-grade fever, general erythroderma, diarrhea, hyperbilirubinemia and evaluation of liver enzymes. A diagnosis of TA- GVHD was suspected. The patients had no response to any therapy and expired 5 days later. TA-GVHD is uncommon complication; there is no effective therapy and high mortality. Since TA- GVHD can be prevented by irradiating blood products with 2,500- 3,500 rads, patients at high risk should receive irradiated blood products only.
Arthritis, Gouty ; diagnosis

We study characteristic of 306 cord blood samples collected at Hung Vuong hospital from 1997 year to 2000 year. The results were the following: as compared with foreign authors’ results we revealed that the median volume of Vietnamese newborn’s cord blood was smaller, there were not significantly different on the median number of nucleated cells, the median number of CD34+ stem cells, the percentage of CD34+ cells as compared with mononuclear cells and the value of the use of cord blood for transplantation; the average percentage of immunologic makers on cord blood cells and peripheral blood cells and in our study was comparable with other author’s results but the percentage of CD2+ and CD19+; hematologic indexes of cord blood in our study were not significantly different as compared with other but the number of granulocytes, red blood cells and hematocrite were smaller; the incidence of infectious diseases was the following: bacterial infection 5.9%; viral infection: HbsAg(+) : 1.79%; anti HCV(+): 1.34%; anti HIV(+): 0.44%; VDRL(+): 0%; anti HTLV-1.2: 0; anti CMV IgM: 0.90%. Anti HBc IgM: 5.3%. Conclusion as compared with foreign authour results we revealed that the median volume of Vietnamses newborn’s cord blood was smaller and there were not significantly different on the median number of nucleated cells, the median number of CD34+ stem cells, the percentage of CD34+ cells as compared with mononuclear cells and the average percentage of immunologic makers. So it is useful in transplantation to treat malignant and congenital diseases in Vietnam.
Fetal Blood ; cells

On January and June, 2002 four Cord Blood Transplantations were implemented at HCMC Blood Transfusion Hematology Center with ALL, AML, CML and (Thalassemia, where a relation and three unrelation, Results: ANC>0.5x109 L/L: 21 days (relation) and 45 days (unrelation). Platelet count of >50x109 L/L: 27 days (relation) and 60 days (unrelation).
Cord Blood Stem Cell Transplantation ; Fetal Blood