59 batches of purified tetanus antitoxin and 20 batches of anti-rabies serum (1997-2001) produced at IVAC Nha Trang were tested for: pH, total nitrogen, merthiolate content, sodium chloride content. In general, all the batches met the minimum requirements of the manufacture. However in order to improve the quality of the sera additional requirements should be set up
Tetanus Antitoxin ; Serum

Background Nha Trang Institute of Vaccines Medical Biologicals has produced successfully vaccine for human influenza A/H5N1 in embryonic chicken eggs. Effectiveness of the vaccine immune response is highly dependent on the type of adjuvant used.\r\n', u'Objective: To study adjuvants for preparing A/H5N1 influenza vaccine.\r\n', u'Subjects and methods: The study used a comparative approach on capacity to increase the immune response of various adjuvants AIPO4, Chitosan and Freund tested in mice. Experiment of erythrocyte agglutination reaction is used to measure levels of antibody response in mice blood. The process of weight gain in mice after injection of vaccine adjuvant mixture is monitored.\r\n', u'Results: HA antibody level of the vaccine adjuvant mixture is higher than of the vaccine without adjuvant. Capacity of stimulating immune response of Chitosan adjuvant is higher than AlPO4. Repeat injection on day 20th is suitable for vaccine with AlPO4or vaccine without adjuvant. Repeat injection is after day 20th for vaccine with Chitosan or Freund depending on the amount of residual antibody in the blood. \r\n', u'Capacity of stimulating immune response of Freund adjuvant is the best in the 3 adjuvants but the safety of this adjuvant is low\r\n', u' Freund adjuvant should be used only for animals. When using Freund adjuvant to induce immunity to animals it should determine the amount of antibodies causing immune before repeating.\r\n', u'Conclusion: Freund adjuvant should be used only for animals. \r\n', u'\r\n', u'
Influenza A Virus ; H5N1 Subtype

Introduction: There are thousands of diabetes sufferers worldwide. In addition, there is an increased trend in Vietnam due to economic development, increased population, lifespan, and changing lifestyle. Insulin is a hormone, which is a natural protein. It plays an important role in the transformation of glucose in human and animal blood. Note, while insulin has not been produced in Vietnam, the production of recombinant Proinsulin is a premise for insulin. This study is based on a successful design of pET - 28a (+) Vector with recombinant Proinsulin codified gene.\r\n', u'Objectives: To define the human proinsulin codified gene and study the optimum conditions for the expression of proinsulin. \r\n', u'Subjects and method: To discover the appropriate conditions for the expression of proinsulin, including initial cell density, cultural temperature, IPTG concentration, and expression time. The product of the expression was confirmed by Sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SOS - PAGE) and reconfirmed by Western blotting with His - Tag antibody. \r\n', u'Results: Proinsulin expression was successfully proved by SOS - PAGE and Western blotting. Four appropriate conditions for the expression were confirmed as highlighted in the Conclusion. \r\n', u'Conclusion: The appropriate conditions for expression of proinsulin: cell density was 00600 0.6 - 1.0; the cultural temperature was 300C; IPTG concentration was 0.4 mM; the length of the culture time was 20 hours. \r\n', u'
Expression ; Proinsulin ; E.Coli ; Appropriate conditions

Between 1996 and 1998, 31 cases of primary extranodal malignant non-Hodgkin's lymphoma were studied immunohistochemically. Immunohistochemical study showed that the majority of the cases were of B-cell type: 19 cases (73.07%) and only 3 cases (11.53%) were of the T-cell type. According to the Working Formulation system, the most common histologic type was diffuse, large cell type (61.29%). The histologic grade was moderate grade: 23 cases (74.19%), high grades: 5 cases (16.13%).
Immunohistochemistry ; Lymphoma, Non-Hodgkin

40 patients diagnosed as cholangiome of which 9 patients diagnosed by biliary cell, 13 patients diagnosed by CT scanner, and 18 patients diagnosed by operation. The biliary obstruction in the general hepatic tube occurred in 95% of patients in which the lesion involved in the 2 branches of liver was 75%. The image of cholangiome in the film of cholangiography found that biliary fair stenosid (62,5%).
Adenoma, Bile Duct ; diagnosis

50 patients with the odontopathy and odontic advantages of technique of the three dimensional obturation/warm vertical condensation of gutta- percha included easy implementation, close obturation of the accessory root canals and high rate of healing the lesion of endodontic origin and limitations included no application for the odontic pedicle that have not been closed.
Endodontics ; methods

The study was conducted on 593 children, 325 salt samples, 60 urinary samples and 30 fresh water samples, using the descriptive epidemiological method. Ca, Na, K, Cl, Cyanide, Fl, Cu, Pb and Iode contents were quantified. The data was processed by statistic method. The results showed that Ca and Fl contents were within acceptable limit. Iode was provided sufficiently. The content of cyanide, an anti-thyroid agent, was higher than acceptable limit
Goiter ; Chemistry ; water ; epidemiology

An investigation were carried out in 642 families and 51 water samples. The results: number of used mine-water families occupied high rate 77,24%. The CN content in drinking water source is 7,14+/- 1,9, 10 times higher than this the limited standard, the rate of goiter : 53,91%, the rate of goiter: among the used mine- water family is higher 2,28 times than this of the families does not use mine- water.
Water ; Goiter ; environment

Root canal treatment in three dimensions is the best technique in the world. In this study, 30 teeth with 81 rood canals are obturated with heat carries. The propotion of lateral canal is 30.86% and the propotion of success is 92.59%. It is the advantage of the technique. This technique is applicable in our working condition with heat carries and bunsen burner
Dental Pulp Cavity ; Dental Pulp Diseases ; therapeutics ; Root Canal Obturation

35 patients with internal hemorrhoid of II, III, IV grade were treated by simple technique in a short duration and thourough operation without complicate equiments, the traditional formule “kho tri tan E” in combining with Vietnamese traditional herbal medicines gave preliminary satisfied efficacy through the evaluation of 2 main criteria of treatment as bleeding severity and the fall of hemorrhoid knot. Before the treatment, 100% of patients had got bleeding of diverse levels. After the treatment, there was 14.3% of patients still had got mild bleeding. In 100% of treated cases, the whole of hemorrhoid knots falled down. No complication occurred.
Medicine, Traditional ; Therapeutics ; Hemorrhoids