The amount of lymphocyte B and the responding capacity to create antibody of activated lymphocyte B are two important parameters to assess the humoral-immunity responding capacity of healthy and unhealthy bodies. The amount of lymphocyte B were determined in 22 healthy Katu minority living in Nam Dong district, Hue city with technique direct immuno-fluorescence, and 64 Katu people have HbsAg(-) and anti-HbsAg(-), who were studied the responsive capacity to create antibody against hepatitis B vaccine with technique ELISA. The control group are Kinh people of the same criterion. Results: The amount of lymphocyte B in 1mm3 blood: 354.9  101.1; lower than that of the Kinh people of the same age, same occupation, with p < 0.05. The responding capacity to create antibody against hepatitis B vaccine at good standard, 84.4%; that is higher than that of the Kinh people, however with p > 0.05
Lymphocyte Count ; hepatitis B ; Antigens, Bacterial ; blood ; Antibody Formation

In order to evaluate the resistant capacity of humoral immunity of severe protein calorie mainourished children, the author studied: Levels of IgG, IgA, IgM in serum of 111 severe protein calorie malnourished patients. The IgG, IgA levels of protein calorie malnourished patients are higher than that of the control group, but the IgG level is lower than that of the control group at the same age with p<0.01. Before and after vaccination, the antibody titre of the protein calorie malnourished patients is lower than the control group. After vaccination, antibody titre against cholera antigen and typhoid antigen of the protein calorie malnourished children increases that proves the responding capacity to create antibody against these antigens is still good
Antibody Formation ; Child ; Malnutrition

Using high-immunological techniques (immunoturbidimetry and ELISA), we quantify: IgG, IgA, IgM in 60 persons, of whom 36 are male and 24 are female from 18 to 45 years old with good health. Results: IgG (mg%): 1707247; IgA (mg%): 23665; IgM (mg%): 27788.5. IgE in serum of 62 persons, of whom 38 are male and 24 are female from 18 to 45 years old with good health and no allergic prehistory: IgE (ng/ml): 435171. Comparing to the results found by some authors, who studied by using RID technique, it is shown that: their IgG and IgM quantity are lower than ours, but the IgA quantity is higher than ours
Adult ; Immunoglobulin G ; Immunoglobulin A ; Immunoglobulin M ; Immunoglobulin E ; Serum

Introduction: Hepatitis C is a dangerous disease and if left untreated can have serious consequences to the patients. While hepatitis C is difficult to treat, its vaccine is still unavailable. Previous studies have showed that there is a low rate of drug users having HCV tests. This means HCV infections does not have any great importance attached to it amongst the drug user group.\r\n', u'Objectives: To discover the status of HCV infection among drug users in Hanoi and to analyse the associated factors. \r\n', u'Subjects and methods: This crossover descriptive study was conducted in Hanoi. Research objects consisted of only men within the 16 - 25 years old age group, who used heroin from 09/2005 to 12/2006. These participants were interviewed and tested for HIV, HCV and HBV.\r\n', u'Results: HCV prevalence is 35.1 %. Especially, the research has indicated that 100% of HIV infected cases have also contracted HCV. This co - infectious status can be a challenge to subjects when they experience ARV treatment. The mean age of surveyed participants is 19. 83.6% of them have attended secondary school. 30.1 % have taken part in illegal earning activities. \r\n', u'Conclusion: HCV infection was discovered in the early drug injection stage. The proportion of HIV infection among injecting drug users is 28.24 times compared to the smoking drug users.\r\n', u'
HeV ; HIV ; Injecting drug user

Background: Hue city is a cultural and tourism center of The middle and the whole country. Beside developing tourism services, food processing establishments, traditional specialities, eating and drinking services especially street food services more and more develop plentifully and multiform, satisfy daily demand of consumers. Objectives: Assessment on improvement level on some targets of street food safety and hygiene after 2 years intervention. Subjects and method: All street food processing establishments in 3 wards: V\u0129nh Ninh, Ph\xfa H\u1ed9i, V\ufffd?D\ufffd?of Hue city. Method: Cross-sectional study with comparison before and after intervention. Results: Kitchen utensils samples have met hygiene requirements and the proportion of food samples without borax both increase. The infection rate of bacteria in cook\ufffd?hands, kitchen utensils, cooked food were improved after 2 years carried out targeted model. Clean water for processing street food have not enough although 100% households use running water. The proportion of people were trained about food safety and hygiene knowledges and health examination increase. The situation of using food colourings and poisonous additives decrease remarkably. Salesclerks have more consciousness of preserving and covering food. 86,4% of food processing establishments have recycle bins obtain requirement to reduce polluted food. Conclusion: Street food is an important stage of food supplying network in 3 wards above. Somewhere having interest of Government and local authorities, Steering committee have efficient activities street food model develop conveniently.
Food Safety/ methods

Background: The combination medical between army and pepople in protection, healthcare for people and army in the whole country was a great policy of The Party and State. Objectives: Evaluate result of combination between armed forces in epidemic control and preventive medicine programs at mountainous and coastal areas of Thua Thien Hue in 2006. Studying knowledge, attitude, practice of army medical officers and men canvassed masses about epidemic control and preventive medicine programs. Subjects and method: Officers, men, army medical officers in 41 communes (28 coastal communes and 13 mountainous communes) of 5 district have combination medical between army and pepople. Using epidemiology method to description, analysis, cross-sectional study. Results: In 2006, home-flies spraying was carried out in 18 out of 20 markets; 1,030 all size restaurants and 18 residential areas of 5 districts. The number of chemically treated latrines in high epidemic areas was accounted fof 15,1%. A population-based latrine construction project was initiated to build 838 new latrines, contributing to minimize the invironmental pollution. 9,17% of waterwells and water tanks were hygienically treated; 11,434 people used clean water source; and 46,436 people washed their hands with soap. Studying knowledge, attitude, practice on preventive medicine programs of army medical officers showed very good results. Conclusion: The combination medical between army and pepople in preventive medicine, epidemic control gave wide efficiency for mountainous and coastal communes of Thua Thien Hue. It actively supported for Health station of 28 coastal communes and 13 mountainous communes in preventive medicine work.
Community Health Services/ ethics ; manpower ; Health Knowledge ; Attitudes ; Practice ;

Background: At present, management and supervision on food safety and hygiene quality have many difficulties because supervisory system from province to district, commune is still insufficient. Objectives: Assessment on food safety and hygiene situation at Thua Thien Hue province, propose solutions to intensify State management on food safety and hygiene quality and step by step enhance efficiency of food safety and hygiene activities in the next years. Subjects and method: Food processing establishments at Thua Thien Hue province. Method: Inspection in Action months for food safety and hygiene, supervision usually on food processing establishments were chosen accidentally in area. Food samples were inspected indexes of microorganisms, physicochemical have met the criteria of Ministry of Health. Results: The proportion of food processing establishments and cafeterias which have not met the regulations on food safety and hygiene was decreasing from 40,93% to 27,45%. Also in 2002-2006, 36,71% of kitchen utensils and the cooks\ufffd?hands failed to meet the regulations. Regarding to microorganisms, the indices of ice-cream, bean sweet porridge and soft drinks had improved remarkably. The index of ice had been decreased from 33,02% to 22,56%. 45,25% of bowls and plates didn\u2019t meet chemical and physical criteria. 68,4% of food colourings didn\u2019t meet criteria because inorganic and industrial colourings were used. Traditional foods still contain borax although the prevalence is decreasing from 65% to 35%. Ice cream and bean sweet porridge cooked with saccharine were 37,34%. Conclusion: Understanding of producers and consumers about food have still many limitations.
Food Safety/ methods ;

Background: Recently, microplate almar blue assay has been used commonly in detecting gen related to tuberculosis drug resistance, which provides results after 5-7 days with lower cost compared to traditional methods. Objective: To evaluate the application of microplate almar blue assay for rapid detection of drug resistance of M. tuberculosis strains. Subject and Method: A microplate-based assay which uses Alamar blue reagent - an oxidation reduction dye (MABA), was used for the determination of the anti-tuberculosis drug (isoniazid-INH, rifampicine-RIF, streptomycine-STR and ethabuton=EMB) resistance of 96 M. tuberculosis strains isolated from Vietnamese patients in comparison to those obtained by conversional method. Result: MABA showed to have high sensitivity and specificity in testing the sensitivity to individual anti-tuberculosis drugs (from 82.4% for STR to 93.3% for - INH and from 82.5% for EMB to 98.4% for STR; respectively), as well as for the multi-drug resistant M.tuberculosis (86.4% of sensitivity), highly correlated with the result determined by proportion method. Conclusion: MABA reveals the advantage in shortening test time, in simple performance and lower cost compared with the conversional culture based methods.
Microplate almar blue assay ; M. tuberculosis ; drug resistance

Background: HIV/AIDS pandemic really become danger to mankind on the earth. Objectives: Description epidemiological characteristics of HIV/AIDS patients. Studying clinical expression, learning aboutdemands of infected patients and community care for infected patients. Subjects and method: 71 HIV/AIDS people in Thua Thien Hue were studied in 2006. Using descriptive method to give epidemiological characteristics and behaviour of HIV/AIDS people. People were determined HIV/AIDS infection based on the criteria of Ministry of Health. The information were collected by target study. Results: HIV/AIDS people mainly from the age of 20 to 39 (83,10%), males were 52,10% and in various levels of education and all kinds of occupation. The rate of sexually transmitted infection was 84,5% and infection transmission was 15,5%. The rate of sexually transmitted infection within the last 12 months was 57,7%. 63,4% of infected people used condom while having sex with their spouses or partners. 1,4% of infected people using drug infection within the last month. 38% of the infected people had manifestations of AIDS, 77,8% of HIV/AIDS were treated with antiviral drugs. Families and communities had positive attitudes and behaviors to the HIV/AIDS people. Infected people being remoted from the society was 12,7%, 88,7% of them receiving supports from their communities such as disease treatment, spirit and material supports and job opportunities. Conclusion: Strengthen communication activities to change behavior of HIV/AIDS people and in communitiy to reduce alienation, discrimination for infected people. Execute socialization in care and support for HIV/AIDS people.
HIV/ immunology ; isolation & ; purification ; pathogenicity ; Community Health Services/ organization & ; administration ; Epidemiologic Methods ;

Background: Acute Respiratory Infection (ARI) is a common disease in developing countries. Morbidity and mortality of ARI are high, especially among children under 15 years old. Objectives: To describe socio-graphic factors, seasonal patterns, risky areas and determine the morbidity and mortality rates of acute respiratory infections in Thai Binh province. Subjects and method: This retrospective study reviewed the medical records of 4,585 hospital admitted patients who were diagnosed with ARI including upper and lower respiratory infections such as sore throat, pharyngitis, bronchitis, pneumonia and bronchitis-pneumonia at 8 district hospitals and 1 provincial hospital in Thai Binh province during 2002-2005. The selected medical records were based on the available check list and two standard screening tests. Results:Morbidity and mortality of ARI in Thai Binh province were 61.6 and 0.52, respectively. ARI mainly occurred among children under 5 years old, of which the highest mortality was among those under 12 months of age. Male children were at higher risk of acquiring ARI, but less prone to death than female. Occupation did not significantly associate with the risk of ARI. The morbidity increased sharply during inter-season, e.g. March and October. Thai Binh city, Kien Xuong and Tien Hai district were reported with the highest morbidity in accompany of the high mortality as consequences. Conclusion: The prevention and control methods were recommended to annually focus on the male children aged less than 5 years old during March and October in Thai Binh city, Kien Xuong and Tien Hai district.
morbidity ; mortality ; acute respiratory infections